LOCUS       sequence-391761-        7825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing an antigen targeting Neuroligin-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7825)
  TITLE     Trimmer lab antigen plasmids
REFERENCE   2  (bases 1 to 7825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NGL-1-myc/pEGFP-N1
FEATURES             Location/Qualifiers
     source          1..7825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1163..1618
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1250..1269)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1460..1481
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        1936..2315
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2316..2519
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2470..2490
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2550..2594
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             2595..2618
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      3110..3119
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4842..4844,4845..5555)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4884..4905)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5145..5164)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5492..5513
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5784..6406
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5983..6002)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(6430..6450)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6435..6764
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6615..6750
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6802..6821)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6803..6821)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6835..6851)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6835..6851)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6848..6870)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6859..6875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6883..6889,6890..6907,6908..6913))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6928..6949
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7066..7083)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7237..7825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7317..7336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     1201 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     1261 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     1321 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     1381 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     1441 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     1501 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     1561 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgatctat acattgaatc
     1801 aatattggca attagccata ttagtcattg gttatatagc ataaatcaat attggctatt
     1861 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
     1921 tatgaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt
     1981 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     2041 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     2101 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     2161 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg
     2221 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc
     2281 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca
     2341 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     2401 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg
     2461 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     2521 gtttagtgaa ccgtcagaat taattcacca tgtctgcact tctgatccta gctcttgttg
     2581 gagctgcagt tgctgactac aaagacgatg acgacaagct tgcggccgcg aattcatcga
     2641 tagatctgca aaagttggat gatgtagacc cattggttac tactaacttt ggcaaaataa
     2701 ggggaattaa gaaagaactc aataatgaaa ttttggggcc tgtcattcag tttcttgggg
     2761 ttccatatgc tgctccacca acaggagaac atcgatttca gcctccagaa ccaccatctc
     2821 cctggtctga tatccggaat gccactcagt ttgctcctgt atgtccccag aatatcattg
     2881 atggcagatt gcctgaagtt atgcttcctg tgtggttcac taataacttg gatgtggttt
     2941 cctcatatgt gcaagaccag agtgaagact gtctatactt aaacatctat gtcccaactg
     3001 aagatgtaaa aagaatatcc aaggaatgtg ccagaaaacc cggcaagaaa atatgtagaa
     3061 aaggagatat tcgggacagt gggggtccca aaccagtgat ggtgtacatc catggtggct
     3121 cttacatgga aggtactgga aatctatatg atgggagtgt gttggcaagc tacggtaatg
     3181 tgatcgtcat cacagtcaac tatcggcttg gggtacttgg cttcttgagc acaggggatc
     3241 aggctgccaa aggaaactac gggctccttg acctcatcca ggccctcaga tggaccagcg
     3301 agaacattgg gttctttggt ggtgacccct tgcgaatcac cgtgtttgga tcaggcgctg
     3361 ggggttcatg tgtcaatctg ctgactttat cccattattc tgaaggtaac cgttggagca
     3421 attcaaccaa aggacttttt caacgagcaa tagctcagag tggaacagcc ctttccagct
     3481 gggctgttag tttccagcct gcaaaatacg ctagaattct agccacaaaa gttggctgca
     3541 atgtgtcaga tacagtagag ttagtagaat gcctgcagaa gaagccttac aaagaacttg
     3601 ttgatcaaga tgttcaacca gcccgatacc acatagcctt tggacctgtg atcgatggtg
     3661 atgtaatacc agatgaccct cagatactga tggaacaagg agagttcctc aattatgata
     3721 tcatgttagg agttaaccaa ggggaagggt tgaagtttgt ggaaaacata gtagatagtg
     3781 atgatggtgt atcagccagt gattttgact ttgctgtctc taattttgtt gataatttat
     3841 atggatatcc tgaaggcaaa gatgttttga gagaaaccat taaattcatg tatactgact
     3901 gggctgatcg ccataaccct gaaactagaa ggaagacatt gttggctttg tttacagacc
     3961 atcagtgggt agcacccgct gtggctacag cagaccttca ctcgaacttt ggctcaccta
     4021 catacttcta tgccttttat catcattgcc aaactgacca agttccagct tgggctgatg
     4081 cagctcatgg ggatgaggtt ccctatgtat tgggaatccc catgattggc cctacagagt
     4141 tatttccttg caatttctcc aagaatgatg tgatgttgag tgctgtagta atgacatact
     4201 ggacgaattt tgctaaaacc ggtgacccaa atcaaccagt tcctcaagac acaaaattca
     4261 ttcataccaa acccaaccgc tttgaagaag tagcatggac cagatattcc cagaaagacc
     4321 aactttatct ccatattgga ttaaaaccga gagttaaaga acattacaga gccaataagg
     4381 taaatctctg gttggagctg gtacctcatc tgcataatct caatgacatt tctcagtata
     4441 cctcgacaac aactaaagtg ccatcaacag acatcactct cagacctaca aggaaaaatt
     4501 ccactccagt cacatcagcc tttcccactg ccaagcagga tgatcccaaa caacaaccaa
     4561 gtcccttctc ggtagatcaa agggactact ccacagagct aagtgtcaca atcgcagtgg
     4621 gagcctctct gctgtttctg aacatcttgg cttttgcagc cctatactac aagaaagaca
     4681 agaggagaca tgatgtccac cggaggtgca gccctcagcg caccacgacc aacgacctaa
     4741 cccatgctcc agaagaggaa attatgtctc tccaaatgaa gcacactgac ttggatcacg
     4801 agtgtgagtc catccatcca catgaggtgg ttcttcggac cgtgagcaag ggcgaggagc
     4861 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     4921 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga
     4981 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg
     5041 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     5101 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     5161 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     5221 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     5281 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     5341 tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc
     5401 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccaagc
     5461 ttagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     5521 ccgggatcac tctcggcatg gacgagctgt acaagcggac cgcctgcccc ccagattata
     5581 ctcttgctat gaggaggtca ccggatgatg ttcctttaat gacacctaac accatcacaa
     5641 tgattcccaa cactatacca gggattcagc ccttacatac attcaacaca tttactggag
     5701 gacagaataa tacactgccc cacccccacc ctcaccccca ttcacattca acaaccaggg
     5761 tatagtcgac tctagaggat cccgggtggc atccctgtga cccctcccca gtgcctctcc
     5821 tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca
     5881 tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga
     5941 gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct
     6001 ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc
     6061 tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat
     6121 ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct
     6181 aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca
     6241 ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag gacgtccaga
     6301 cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc ttgcttggca
     6361 ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattgggt acgcggccag
     6421 cttggctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     6481 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     6541 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     6601 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6661 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6721 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ctcgaggaac
     6781 tgaaaaacca gaaagttaat tccctatagt gagtcgtatt aaattcgtaa tcatgtcata
     6841 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6901 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6961 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7021 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7081 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7141 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7201 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7261 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7321 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7381 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7441 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7501 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7561 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7621 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7681 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7741 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7801 tacgcgcaga aaaaaaggat ctcaa
//
