LOCUS       sequence-391766-       12447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing an antigen targeting the Menke's disease copper 
            ATPase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12447)
  TITLE     Trimmer lab antigen plasmids
REFERENCE   2  (bases 1 to 12447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: h-Menkes
FEATURES             Location/Qualifiers
     source          1..12447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1565..2356,2357..2425))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2188..2207
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2426..2530)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        2569..2948
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3462..4478
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4402..4424
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4486..4505
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             6745..6774
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(9351..9367)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9352..9368)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(9391..9409)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9392..9409)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9429..9446)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9435..9659
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9705..10133
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9792..9811)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10002..10023
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10142..10162)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10147..10476
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10327..10462
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10389..10408
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             10543..11337
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10597..10616)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11207..11226
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11511..11632
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11548..11567)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11602..11621
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11771..11993
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             join(12041..12447,1..544)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSKLVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 ttttgaaaac aaatactcta tgaggattta tgagtggtta ttaaaagaac taacacaaaa
       61 gaaaactcac aaggcaaata tagagattag ccttgatgaa tttaagttca tgttaatgct
      121 tgaaaataac taccatgagt ttaaaaggct taaccaatgg gttttgaaac caataagtaa
      181 agatttaaac acttacagca atatgaaatt ggtggttgat aagcgaggcc gcccgactga
      241 tacgttgatt ttccaagttg aactagatag acaaatggat ctcgtaaccg aacttgagaa
      301 caaccagata aaaatgaatg gtgacaaaat accaacaacc attacatcag attcctacct
      361 acataacgga ctaagaaaaa cactacacga tgctttaact gcaaaaattc agctcaccag
      421 ttttgaggca aaatttttga gtgacatgca aagtaagtat gatctcaatg gttcgttctc
      481 atggctcacg caaaaacaac gaaccacact agagaacata ctggctaaat acggaaggat
      541 ctgaggttct tatggctctt gtatctatca gtgaagcatc aagactaaca aacaaaagta
      601 gaacaactgt tcaccgttac atatcaaagg gaaaactgtc catatgcaca gatgaaaacg
      661 gtgtaaaaaa gatagataca tcagagcttt tacgagtttt tggtgcattc aaagctgttc
      721 accatgaaca gatcgacaat gtaacagatg aacagcatgt aacacctaat agaacaggtg
      781 aaaccagtaa aacaaagcaa ctagaacatg aaattgaaca cctgagacaa cttgttacag
      841 ctcaacagtc acacatagac agcctgaaac aggcgatgct gcttatcgaa tcaaagctgc
      901 cgacaacacg ggagccagtg acgcctcccg tggggaaaaa atcatggcaa ttctggaaga
      961 aatagcgctt tcagccggca aaccggctga agccggatct gcgattctga taacaaacta
     1021 gcaacaccag aacagcccgt ttgcgggcag caaaacccgt acttttggac gttccggcgg
     1081 ttttttgtgg cgagtggtgt tcgggcggtg cgcgcaagat ccattatgtt aaacgggcga
     1141 gtttacatct caaaaccgcc cgcttaacac catcagaaat cctcagcgcg attttaagca
     1201 ccaacccccc cccgtaacac ccaaatccat actgaaagtg gctttgttga ataaatcaga
     1261 tttcgggtaa gtctcccccg tagcgggttg tgttttcagg caatacgcac gctttcaggc
     1321 atacctgctt tcgtcatttt gttcagcgct cgtaccaggg ccatagcctc cgcaacctga
     1381 ccatcgtagt cacgcagcgt cagtgaaccc ccgaacagag atctgacgct cagtggaacg
     1441 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1501 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1561 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1621 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1681 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1741 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1801 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1861 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1921 cattcagctc cggttcccag cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1981 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2041 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2101 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2161 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2221 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2281 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2341 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2401 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2461 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2521 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgtacctcga cattgattat
     2581 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     2641 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     2701 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     2761 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     2821 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     2881 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     2941 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
     3001 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
     3061 gggggggggg ggcgcgccng ncgggggggg gggggggggg gggggggggg gggggggggg
     3121 gggggggggg gggggggggg ggnggngggg gggggggggg gggggggggg gggggggggg
     3181 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg ggcccscrgy
     3241 kkkkkkkkgk tktktktttt tktrrkrtkk gtgggggggg gggggggggg gggggggggg
     3301 gggggcgcgc gccgggsggg gsggggcggg gcgaggggcg gggcggggcg aggcggagag
     3361 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
     3421 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc
     3481 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
     3541 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
     3601 tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg
     3661 agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
     3721 gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc
     3781 tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg
     3841 gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag
     3901 ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg
     3961 agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc
     4021 cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg
     4081 agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga
     4141 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
     4201 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
     4261 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     4321 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
     4381 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     4441 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     4501 ttgtgctgtc tcatcatttt ggcaaagaat tcctcgaagc ttggtaccga gctcggatcc
     4561 actagtaacg gccgccagtg tgctggaatt ctgcagatcc accgcgggaa ttcgattgct
     4621 ctgagcatag gatagagaaa ccaggaatgt aatgaggaaa tcaaaatgga cccaagtatg
     4681 ggtgtgaatt ctgttaccat ttctgttgag ggtatgactt gcaattcctg tgtttggacc
     4741 attgagcagc agattggaaa agtgaatggt gtgcatcaca ttaaggtatc actggaagaa
     4801 aaaaatgcaa ctattattta tgaccctaaa ctacagactc caaagaccct acaggaagct
     4861 attgatgaca tgggctttga tgctgttatc cataatcctg accctctccc tgttttaact
     4921 gacaccttgt ttctgactgt tacggcgtca ctgactttgc catgggacca tatccaaagc
     4981 acattgctga agaccaaggg tgtgacagac attaaaattt accctcagaa aagaactgta
     5041 gcagtgacaa taatcccttc tatagtgaat gccaatcaga taaaagagct ggttccagaa
     5101 ctcagtttag atactgggac actggagaag aagtcaggag cttgtgaaga tcatagtatg
     5161 gctcaagctg gtgaagtcgt gctgaagatg aaagtggaag ggatgacctg ccattcatgt
     5221 actagcacta ttgaaggaaa aattgggaaa ctgcaaggtg ttcagcgaat taaagtctcc
     5281 ctggacaatc aagaagctac tattgtttat caacctcatc ttatctcagt agaggaaatg
     5341 aaaaagcaga ttgaagctat gggctttcca gcatttgtca aaaagcagcc caagtacctc
     5401 aaattgggag ctattgatgt agaacgtcta aagaacacac cagttaaatc ctcagaaggg
     5461 tcacagcaaa ggagtccatc gtataccaat gattcaacag ccactttcat cattgatggc
     5521 atgcattgta aatcatgtgt gtcaaatatt gaaagtactt tatctgcact ccaatatgta
     5581 agcagcatag tagtttcttt agagaatagg tctgccattg tgaagtataa tgcaagctca
     5641 gtcactccag aatccctgag aaaagcaata gaggctgtat caccggggct atatagagtt
     5701 agtatcacaa gtgaagttga gagtacctca aactctccct ccagctcatc tcttcagaag
     5761 attcctttga atgtagttag ccagcctctg acacaagaaa ctgtgataaa cattgatggc
     5821 atgacttgta attcctgtgt gcagtctatt gagggtgtca tatcaaaaaa gccaggtgta
     5881 aaatccatac gagtctccct tgcaaatagc aatgggactg ttgagtatga tcctctacta
     5941 acctctccag aaacgttgag aggagcaata gaagacatgg gatttgatgc taccttgtca
     6001 gacacgaatg agccgttggt agtaatagct cagccttcat cggaaatgcc gcttttgact
     6061 tcaactaatg aattttatac taaagggatg acaccagttc aagacaagga ggaaggaaag
     6121 aattcatcta agtgttacat acaggtcact ggcatgactt gcgcttcctg tgtagcaaac
     6181 attgaacgga atttaaggcg ggaagaagga atatattcta tacttgtggc cctgatggct
     6241 ggcaaggcag aagtaaggta taatcctgct gttatacaac ccccaatgat agcagagttc
     6301 atccgagaac ttggatttgg agccactgtg atagaaaatg ctgatgaagg agatggtgtt
     6361 ttggaacttg ttgtgagggg aatgacgtgt gcctcctgcg tacataaaat agagtctagt
     6421 ctcacaaaac accgcgggat tctatactgc tccgtggccc tggcaaccaa caaagcacat
     6481 attaaatatg acccagaaat tattggtcct agagatatta tccatacaat tgaaagctta
     6541 ggttttgaag cttctttggt caagaaggat cggtcagcaa gtcacttaga tcataaacga
     6601 gaaataagac aatggagacg gtcttttctt gtgagtctgt ttttctgtat tcctgtaatg
     6661 gggctgatga tatatatgat ggttatggac caccactttg caactcttca ccataatcaa
     6721 aacatgagta aagaagaaat gatcgaacaa aaactgattt ctgaagagga tctgaacctt
     6781 cattcttcta tgttcctgga gcgccagatt cttccaggat tgtctgttat gaatttgctg
     6841 tcctttttat tgtgtgtacc tgtacagttt ttcggaggct ggtacttcta cattcaggct
     6901 tataaagcac tgaagcataa gacagcaaat atggacgtac tgattgtgct ggcaaccacc
     6961 attgcatttg cctactcttt gattattctt ctacttgcaa tgtatgagag agccaaagtg
     7021 aaccctatta ctttctttga cacaccccct atgctgtttg tgtttattgc actaggccga
     7081 tggctggaac atatagcaaa gggcaaaaca tcagaggctc ttgcaaagtt aatttcacta
     7141 caagctacag aagcaactat tgtaactctt gattctgata atatcctcct cagtgaagaa
     7201 caagtggatg tggaacttgt acaacgtgga gatatcatta aagtagttcc aggaggcaaa
     7261 tttccagtgg atggtcgtgt tattgaagga cattctatgg tagatgagtc cctcatcaca
     7321 ggggaggcaa tgcctgtggc taagaaacct ggcagcacag tgattgctgg ttccattaac
     7381 cagaacgggt cactgcttat ctgcgcaaca catgttggag cagacacaac cctttctcaa
     7441 attgtcaaac ttgtggaaga ggcacaaaca tcaaaggctc ctatccagca gtttgcagac
     7501 aaactcagtg gctattttgt tccttttatt gtttttgttt ccattgccac cctcttggta
     7561 tggattgtaa ttggatttct gaattttgaa attgtggaaa cctactttcc tggctacaat
     7621 agaagtatct cccgaacaga aacgataata cgatttgctt tccaagcctc tatcacagtt
     7681 ctgtgtattg catgtccctg ttcactggga ctggccactc caactgctgt gatggtgggt
     7741 acaggagtag gtgctcaaaa tggcatacta ataaaaggtg gagagccatt ggagatggct
     7801 cataaggtaa aggtagtggt atttgataag actggaacca ttactcacgg aaccccagtg
     7861 gtgaatcaag taaaggttct aactgaaagt aacagaatat cacaccataa aatcttggcc
     7921 attgtgggaa ctgctgaaag taacagtgaa caccctctag gaacagccat aaccaaatat
     7981 tgcaaacagg agctggacac tgaaaccttg ggtacctgca tagatttcca ggttgtgcca
     8041 ggctgtggta ttagctgtaa agtcaccaat attgaaggct tgctacataa gaataactgg
     8101 aatatagagg acaataatat taaaaatgca tccctggttc aaattgatgc cagtaatgaa
     8161 cagtcatcaa cttcgtcttc catgattatt gatgcccaga tctcaaatgc tcttaatgct
     8221 cagcagtata aagtcctcat tggtaaccgg gagtggatga ttagaaatgg tcttgtcatt
     8281 aataacgatg taaatgattt catgactgaa catgagagaa aaggtcggac tgctgtatta
     8341 gtagcagttg atgatgagct gtgtggcttg atagccattg cagacacagt gaagcctgaa
     8401 gcagaactgg ctatccatat tctgaaatct atgggcttag aagtagttct gatgactgga
     8461 gacaacagta aaacagctag atctattgct tctcaggttg gcattactaa ggtgtttgct
     8521 gaagttctac cttctcacaa ggttgctaaa gtgaagcaac ttcaagagga ggggaaacgg
     8581 gtagcaatgg tgggagatgg aatcaatgac tccccagctc tggcaatggc taatgtggga
     8641 attgctattg gcacgggcac agatgtagcc attgaagcag ctgatgtggt tttgataagg
     8701 aatgatcttc tggatgtagt ggcaagtatt gacttatcaa gaaagacagt caagaggatt
     8761 cggataaatt ttgtctttgc tctaatttat aatctggttg gaattcccat agctgctgga
     8821 gtttttatgc ccattggttt ggttttgcag ccctggatgg gatctgcagc aatggctgct
     8881 tcatctgttt ctgtagtact ttcttctctc ttccttaaac tttacaggaa accaacttac
     8941 gagagttatg aactgcctgc ccggagccag ataggacaga agagtccttc agaaatcagc
     9001 gttcatgttg gaatagatga tacctcaagg aattctccta aactgggttt gctggaccgg
     9061 attgttaatt atagcagagc ctctataaac tcactactgt ctgataaacg ctccctaaac
     9121 agtgttgtta ccagtgaacc tgacaagcac tcactcctgg tgggagactt cagggaagat
     9181 gatgacactg cattataaaa ggccatggag agtgctgcca gtttaacttg tcatgcactg
     9241 acacagcatt catgatgtta ccttcacttt tcaaaatatt gtagaaggat ttttctcatg
     9301 ctcttatatt agggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc
     9361 gacctcgagc atgcatctag agggccctat tctatagtgt cacctaaatg ctagagctcg
     9421 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     9481 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     9541 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     9601 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     9661 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     9721 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     9781 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     9841 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     9901 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     9961 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
    10021 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
    10081 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
    10141 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
    10201 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
    10261 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
    10321 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
    10381 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
    10441 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
    10501 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
    10561 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
    10621 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
    10681 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
    10741 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
    10801 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
    10861 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
    10921 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
    10981 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
    11041 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
    11101 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
    11161 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
    11221 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
    11281 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
    11341 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
    11401 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
    11461 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
    11521 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    11581 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    11641 gtaattcagc gatttgcccg agcttgcgag ggtgctactt aagcctttag ggttttaagg
    11701 tctgttttgt agaggagcaa acagcgtttg cgacatcctt ttgtaatact gcggaactga
    11761 ctaaagtagt gagttataca cagggctggg atctattctt tttatctttt tttattcttt
    11821 ctttattcta taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc
    11881 ggaatttaca gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt
    11941 acagataccc acaactcaaa ggaaaaggac tagtaattat cattgactag cccatctcaa
    12001 ttggtatagt gattaaaatc acctagacca attgagatgt atgtctaaat tagttgtttt
    12061 caaagcaaat gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat
    12121 tttatgctgt gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac
    12181 ggtatcgttc acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta
    12241 tggtgtatta gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc
    12301 tttggttaaa ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa
    12361 attagaatta gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat
    12421 aaaatataat ctggaacatg ttaagtc
//
