LOCUS       sequence-391771-        6746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic gene circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Inda-Webb ME, Jimenez M, Liu Q, Phan NV, Ahn J, Steiger C, Wentworth
            A, Riaz A, Zirtiloglu T, Wong K, Ishida K, Fabian N, Jenkins J, 
            Kuosmanen J, Madani W, McNally R, Lai Y, Hayward A, Mimee M, Nadeau 
            P, Chandrakasan AP, Traverso G, Yazicigil RT, Lu TK
  TITLE     Sub-1.4 cm(3) capsule for detecting labile inflammatory biomarkers 
            in situ.
  JOURNAL   Nature. 2023 Aug;620(7973):386-392. doi: 10.1038/s41586-023-06369-x.
            Epub 2023 Jul 26.
  PUBMED    37495692
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06369-x"; journalName: "Nature"; date: 
            "2023-08"; volume: "620"; issue: "7973"; pages: "386-392"
COMMENT     Sequence Label: NO MS-Sensor (30)
FEATURES             Location/Qualifiers
     source          1..6746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1125..1211
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1756..2706)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2754..2976
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3474..3568)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3598..4389,4390..4458))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4221..4240
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4459..4562)
                     /gene="bla"
                     /label=AmpR promoter
     RBS             5017..5028
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5035..6534
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      6642..6728
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata
       61 actttacggg catgcataag gctcgtataa tatattcagg gagaccacaa cggtttccct
      121 ctacaaataa ttttgtttaa ctttgaattc ttcacacagg aaaccggtac catgaatatt
      181 cgtgatcttg agtacctggt ggcattggct gaacaccgcc attttcggcg tgcggcagat
      241 tcctgccacg ttagccagcc gacgcttagc gggcaaattc gtaagctgga agatgagctg
      301 ggcgtgatgt tgctggagcg gaccagccgt aaagtgttgt tcacccaggc gggaatgctg
      361 ctggtggatc aggcgcgtac cgtgctgcgt gaggtgaaag tccttaaaga gatggcaagc
      421 cagcagggcg agacgatgtc cggaccgctg cacattggtt tgattcccac agttggaccg
      481 tacctgctac cgcatattat ccctatgctg caccagacct ttccaaagct ggaaatgtat
      541 ctgcatgaag cacagaccca ccagttactg gcgcaactgg acagcggcaa actcgattgc
      601 gtgatcctcg cgctggtgaa agagagcgaa gcattcattg aagtgccgtt gtttgatgag
      661 ccaatgttgc tggctatcta tgaagatcac ccgtgggcga accgcgaatg cgtaccgatg
      721 gccgatctgg caggggaaaa actgctgatg ctggaagatg gtcactgttt gcgcgatcag
      781 gcaatgggtt tctgttttga agccggggcg gatgaagata cacacttccg cgcgaccagc
      841 ctggaaactc tgcgcaacat ggtggcggca ggtagcggga tcactttact gccagcgctg
      901 gctgtgccgc cggagcgcaa acgcgatggg gttgtttatc tgccgtgcat taagccggaa
      961 ccacgccgca ctattggcct ggtttatcgt cctggctcac cgctgcgcag ccgctatgag
     1021 cagctggcag aggccatccg cgcaagaatg gatggccatt tcgataaagt tttaaaacag
     1081 gcggtttaac ccgggggatc ccatggtacg cgtgctagag gcatcaaata aaacgaaagg
     1141 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     1201 gtaggacaaa tccgccgccc tagacctagg gcctagggta cgggttttgc tgcccgcaaa
     1261 cgggctgttc tggtgttgct agtttgttat cagaatcgca gatccggctt cagccggttt
     1321 gccggctgaa agcgctattt cttccagaat tgccatgatt ttttccccac gggaggcgtc
     1381 actggctccc gtgttgtcgg cagctttgat tcgataagca gcatcgcctg tttcaggctg
     1441 tctatgtgtg actgttgagc tgtaacaagt tgtctcaggt gttcaatttc atgttctagt
     1501 tgctttgttt tactggtttc acctgttcta ttaggtgtta catgctgttc atctgttaca
     1561 ttgtcgatct gttcatggtg aacagctttg aatgcaccaa aaactcgtaa aagctctgat
     1621 gtatctatct tttttacacc gttttcatct gtgcatatgg acagttttcc ctttgatatg
     1681 taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca
     1741 agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg
     1801 ttgtttttgc gtgagccatg agaacgaacc attgagatca tacttacttt gcatgtcact
     1861 caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt
     1921 ttttcttagt ccgttatgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc
     1981 attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc
     2041 aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt
     2101 gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa
     2161 ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat
     2221 atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat
     2281 agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa
     2341 ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa
     2401 cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc
     2461 cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct
     2521 actgatgttc atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct
     2581 tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc
     2641 atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc
     2701 agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag
     2761 tcaatgataa ttactagtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac
     2821 ctttgctgga aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg
     2881 tgtttttttt gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa
     2941 gataaaaaga atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta
     3001 ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa
     3061 aggcttaagt agcaccctcg caagctcggg caaatcgctg aatattcctt ttgtctccga
     3121 ccatcaggca cctgagtcgc tgtctttttc gtgacattca gttcgctgcg ctcacggctc
     3181 tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt atggattcat gcaaggaaac
     3241 tacccataat acaagaaaag cccgtcacgg gcttctcagg gcgttttatg gcgggtctgc
     3301 tatgtggtgc tatctgactt tttgctgttc agcagttcct gccctctgat tttccagtct
     3361 gaccacttcg gattatcccg tgacaggtca ttcagactgg ctaatgcacc cagtaaggca
     3421 gcggtatcat caacaggctt acccgtctta ctgtccctag tactagtgct tggattctca
     3481 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     3541 gtcattactg gatctatcaa caggagtcca agcgagctcg taaacttggt ctgacagtta
     3601 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3661 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3721 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3781 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3841 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3901 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3961 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4021 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4081 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4141 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4201 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4261 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4321 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4381 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4441 gaaatgttga atactcatac tcttcctttt caatattatt gaagcattta tcagggttat
     4501 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4561 cgcacatttc cccgaaaagt gccacctgac gtctgtggct tttatgaaaa tcacacagtg
     4621 atcacaaatt ttaaacagag cacaaaatgc tgcctcgaaa tgagggcggg aaaataaggt
     4681 tatcagcctt gttttctccc tcattacttg aaggatatga agctaaaacc cttttttata
     4741 aagcatttgt ccgaattcgg acataatcaa aaaagcttaa ttaagatcaa tttgatctac
     4801 atctctttaa ccaacaatat gtaagatctc aactacggaa aaactcatct ttgcctcact
     4861 gtcaatttga ctatagatat tgtcatatcg accatttgat tgatagtcat tttgactact
     4921 cattaatggg cataatttta tttatagagt aaaaacaatc agataaaaaa ctggcacgca
     4981 atctgcaatt agcaagacat ctttttagaa cacattaaag aggagaaagg taccatgaga
     5041 gccctggtag tcatccgcct gtcccgcgtc accgatgcta cgacttcacc ggagcgtcag
     5101 ctggagtctt gccagcagct ctgcgcccag cgcggctggg acgtcgtcgg ggtagcggag
     5161 gatctggacg tctccggggc ggtcgatccg ttcgaccgga agcgcagacc gaacctggcc
     5221 cggtggctag cgttcgagga gcaaccgttc gacgtgatcg tggcgtaccg ggtagaccgg
     5281 ttgacccgat cgatccggca tctgcagcag ctggtccact gggccgagga ccacaagaag
     5341 ctggtcgtct ccgcgaccga agcgcacttc gatacgacga cgccgtttgc ggcggtcgtc
     5401 atcgcgctta tgggaacggt ggcgcagatg gaattagaag cgatcaaaga gcggaaccgt
     5461 tcggctgcgc atttcaatat ccgcgccggg aaataccgag gatccctgcc gccgtgggga
     5521 tacctgccta cgcgcgtgga cggggagtgg cggctggtgc cggaccctgt gcagcgagag
     5581 cgcatcctcg aggtgtatca ccgcgtcgtc gacaaccacg agccgctgca cctggtggcc
     5641 cacgacctga accggcgtgg tgtcctgtcg ccgaaggact acttcgcgca gctgcaaggc
     5701 cgcgagccgc agggccggga gtggtcggct accgcgctga agcgatcgat gatctccgag
     5761 gcgatgctcg ggtacgcgac tctgaacggt aagaccgtcc gagacgacga cggagccccg
     5821 ctggtgcggg ctgagccgat cctgacccgt gagcagctgg aggcgctgcg cgccgagctc
     5881 gtgaagacct cccgggcgaa gcccgcggtg tctaccccgt cgctgctgct gcgggtgttg
     5941 ttctgtgcgg tgtgcgggga gcccgcgtac aagttcgccg ggggaggacg taagcacccg
     6001 cgctaccgct gccgctcgat ggggttcccg aagcactgcg ggaacggcac ggtggcgatg
     6061 gccgagtggg acgcgttctg cgaggagcag gtgctggatc tgctcgggga cgcggagcgt
     6121 ctggagaaag tctgggtagc cggctcggac tccgcggtcg aactcgcgga ggtgaacgcg
     6181 gagctggtgg acctgacgtc gctgatcggc tccccggcct accgggccgg ctctccgcag
     6241 cgagaagcac tggatgcccg tattgcggcg ctggccgcgc ggcaagagga gctggagggt
     6301 ctagaggctc gcccgtctgg ctgggagtgg cgcgagaccg ggcagcggtt cggggactgg
     6361 tggcgggagc aggacaccgc ggcaaagaac acctggcttc ggtcgatgaa cgttcggctg
     6421 acgttcgacg tccgcggcgg gctgactcgc acgatcgact tcggggatct gcaggagtac
     6481 gagcagcatc tcaggctcgg cagcgtggtc gaacggctac acaccgggat gtcgaggcct
     6541 gcagcaaacg acgaaaacta cgctgcagca gtttagacac atggcatgga tgaactatac
     6601 aaataacccg ggggatccca tggtacgcgt gctagaggca tcaaataaaa cgaaaggctc
     6661 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     6721 ggacaaatcc gccgccctag acctag
//
