LOCUS       sequence-391790-        7679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  labelling of microtubule plus ends .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7679)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 7679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pBactin-EB3-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..7679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1179..1201
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      1302..1311
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2160..2169
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2166..2876
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      2421..2430
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3075..3209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3112..3131)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3166..3185
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3223..3239)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3223..3239)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3236..3258)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3247..3263
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3271..3277,3278..3295,3296..3301))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3316..3337
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3454..3471)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3625..4213)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3705..3724)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4384..5175,5176..5244))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5007..5026
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5245..5349)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5417..5435
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5473..5495)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5595..5614
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5808..5830
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5822..5839
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5823..5839
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(7640..7679,1..236)
                     /label=chicken beta-actin promoter
ORIGIN
        1 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
       61 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
      121 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      181 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      241 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      301 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      361 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      421 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      481 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      541 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
      601 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
      661 gkgggggggk gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
      721 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
      781 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
      841 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
      901 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
      961 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1021 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1081 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgtccgca
     1141 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1201 cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagcta gcgctaccgg
     1261 actcagatct cgagctcaag cttcgaattc tgcagtcgac tgccaccatg gccgtcaatg
     1321 tgtactccac atctgtgacc agtgaaaatc tgagtcgcca tgatatgctt gcatgggtca
     1381 acgactccct gcacctcaac tataccaaga tagaacagct ttgttcaggg gcagcctact
     1441 gccagttcat ggacatgctc ttccccggct gtgtgcactt gaggaaagtg aagttccagg
     1501 ccaaactaga gcatgaatac atccacaact tcaaggtgct gcaagcagct ttcaagaaga
     1561 tgggtgttga caaaatcatt cctgtagaga aattagtgaa aggaaaattc caagataatt
     1621 ttgagtttat tcagtggttt aagaaattct ttgacgcaaa ctatgatgga aaggattaca
     1681 accctctgct ggcgcggcag ggccaggacg tagcgccacc tcctaaccca ggtgatcaga
     1741 tcttcaacaa atccaagaaa ctcattggca cagcagttcc acagaggacg tcccccacag
     1801 gcccaaaaaa catgcagacc tctggccggc tgagcaatgt ggcccccccc tgcattctcc
     1861 ggaagaatcc tccatcagcc cgaaatggcg gccatgagac tgatgcccaa attcttgaac
     1921 tcaaccaaca gctggtggac ttgaagctga cagtggatgg gctggagaag gaacgtgact
     1981 tctacttcag caaacttcgt gacatcgagc tcatctgcca ggagcatgaa agtgaaaaca
     2041 gccctgttat ctcaggcatc attggcatcc tctatgccac agaggaagga ttcgcacccc
     2101 ctgaggacga tgagattgaa gagcatcaac aagaagacca ggacgagtac ctggatcccg
     2161 ccaccatggt gagcaagggc gaggaggata acatggcctc tctcccagcg acacatgagt
     2221 tacacatctt tggctccatc aacggtgtgg actttgacat ggtgggtcag ggcaccggca
     2281 atccaaatga tggttatgag gagttaaacc tgaagtccac caagggtgac ctccagttct
     2341 ccccctggat tctggtccct catatcgggt atggcttcca tcagtacctg ccctaccctg
     2401 acgggatgtc gcctttccag gccgccatgg tagatggctc cggctaccaa gtccatcgca
     2461 caatgcagtt tgaagatggt gcctccctta ctgttaacta ccgctacacc tacgagggaa
     2521 gccacatcaa aggagaggcc caggtgaagg ggactggttt ccctgctgac ggtcctgtga
     2581 tgaccaactc gctgaccgct gcggactggt gcaggtcgaa gaagacttac cccaacgaca
     2641 aaaccatcat cagtaccttt aagtggagtt acaccactgg aaatggcaag cgctaccgga
     2701 gcactgcgcg gaccacctac acctttgcca agccaatggc ggctaactat ctgaagaacc
     2761 agccgatgta cgtgttccgt aagacggagc tcaagcactc caagaccgag ctcaacttca
     2821 aggagtggca aaaggccttt accgatgtga tgggcatgga cgagctgtac aagtaatagg
     2881 cggccgccat atgcatccta ggcctattaa tattccggag tatacgtagc cggctaacgt
     2941 taacaaccgg tacctctaga taagtaatga tcataatcag ccatatcaca tctgtagagg
     3001 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     3061 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3121 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3181 tcatcaatgt atcttatcat gtctggatca attcgtaatc atgtcatagc tgtttcctgt
     3241 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     3301 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     3361 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     3421 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3481 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3541 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3601 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3661 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3721 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3781 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3841 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3901 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3961 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4021 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4081 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4141 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4201 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4261 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4321 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     4381 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     4441 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4501 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4561 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4621 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4681 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4741 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4801 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4861 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4921 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4981 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     5041 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     5101 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     5161 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     5221 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     5281 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     5341 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     5401 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     5461 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     5521 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     5581 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     5641 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
     5701 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     5761 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     5821 ttgtaaaacg acggccagtg ccaagttggg atctttgcat tggcccacgg ctctcaggat
     5881 ggggatgctc cccttcagca cccggttccc cttggaaact gatggtcctg gctctgtggc
     5941 atggcagtgg cactgtgagg agccccctac cagcagcaca cagtggggtt ggcactgcca
     6001 cgctccggat gccgcgctct gatccaaccc cataatcaag ggaacccgaa ttgccccatc
     6061 attgccccca ccacccccat cctgccgggc cctcacaccc cacgctgcct tctggtgaca
     6121 ttccccagcc caaacccacg gcttcatggc taccgcgggg catttcccat tgccgcccca
     6181 ttatcagctc tgcacacctc ccgctgtacc catgcctcgt ggctgccctt ctttgacgta
     6241 taatcttcta attaataccc ggccttgtca aagtggagca caaacgttaa ttaattcccc
     6301 agcaggcagg taattaacag tgtgactccc tttttgctgc gagtggggct gatacagaga
     6361 gatgtggcac tatggagccc acggggtcct ggcactgggt gcccacggag gtccccatgt
     6421 gctgcagtgt caccgcctcc gaggagacag tattgtccct gcggtgtccc tgcagctcag
     6481 ctctgtccac agggccacct ccagtttgga ggggacacaa tgcagccccg atgcaaccca
     6541 tcctcgcagc atcccaggga caaagacccc actgcaagac cgcacacagg gctgggtccc
     6601 gctcccctaa tatctacagt gcttcttgca tggcccctta atcaatgcag ttaatcagca
     6661 tgcgctcatg caccgctctg gagctgcaaa gcccctcgca gcgctgctca ccaacaccgc
     6721 gcaccgcccc ggcccagcct gcagcacgcg ctgcaaacag gaaagaaaca aaatattgcc
     6781 caaatgtagg caaaggcatt cggctgcctt gacctccgcc gggccgggcc ctgcctgact
     6841 cagctcctta ctcagcgctc gcttcctccc tccggctgcc accgccgcag cgcacaccct
     6901 gacaaagagt ggcccttaac gggctctgag gtgcacccag cagtgcactc agcagtccaa
     6961 gggccggcct ggaggtttgc accgctacgt gctgacatta gcattgaact tggccctggg
     7021 tagtgctgca ggccgggcgg ggtgggtgta gagagtgcag cgcgcgttgc acccggtgcc
     7081 ccttcccctc ccttgcatcc cagcaggctg caccccagca ccaggcccgt gcatgcatgc
     7141 tcctggtgtt attgcagcct ggtgcatgca tgcgtcttga tggtgcagcg ctgtgcatgc
     7201 atcctccttg gtgtgtagca gtctagtgca tgcatacccc tcggtgttat tgctgctctg
     7261 tgcacgcacg ctcattgtat cacttcatcc cagtgcatgc actcacactg gagcgattgc
     7321 tgctcggtgc acgcacactc attgtatcac tgcagctcag tggctgcacg cacaccggtg
     7381 ttattgctgc tcggtgcgtg catgcacatc agtgtcgctg cagctcagtg catgcacgct
     7441 cattgcccat cgctatccct gcctctcctg ctggcgctcc ccgggaggtg acttcaaggg
     7501 gaccgcagga ccacctcggg ggtgggggga gggctgcaca cgcggacccc gctccccctc
     7561 cccaacaaag cactgtggaa tcaaaaaggg gggagggggg atggaggggc gcgtcacacc
     7621 cccgccccac accctcacct cgaggtgagc cccacgttct gcttcactct ccccatctc
//
