LOCUS       sequence-391806-        7478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a tandem (td) red fluorescent reporter mCherry driven 
            by the strong Beta-actin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 7478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pBactin-td-mCherry-CI
FEATURES             Location/Qualifiers
     source          1..7478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1179..1201
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      1254..1263
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2691..2747
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    2874..3008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2911..2930)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2965..2984
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3022..3038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3022..3038)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3035..3057)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3046..3062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3070..3076,3077..3094,3095..3100))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3115..3136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3253..3270)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3424..4012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3504..3523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4183..4974,4975..5043))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4806..4825
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5044..5148)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5216..5234
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5272..5294)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5394..5413
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5607..5629
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5621..5638
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5622..5638
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(7439..7478,1..236)
                     /label=chicken beta-actin promoter
ORIGIN
        1 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
       61 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgrg gggcggggcg
      121 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      181 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      241 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      301 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      361 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      421 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      481 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      541 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
      601 ggtgccccgc ggtgcggggg gggctgcgrg gggaacaaag gctgcgtgcg gggtgtgtgc
      661 gkgggggggk gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
      721 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
      781 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
      841 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
      901 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
      961 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1021 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1081 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgtccgca
     1141 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1201 cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagcta gcagccacca
     1261 tggtcagcaa aggggaagag gataacatgg ccatcatcaa agagttcatg cggttcaagg
     1321 tgcacatgga aggcagcgtg aacggacatg agttcgagat cgaaggagaa ggagagggac
     1381 gcccttacga gggaactcag accgcaaagc tgaaagtgac taagggtggc cctctgccgt
     1441 ttgcgtggga cattctgtcc ccgcaattca tgtatggctc aaaagcctac gtcaaacacc
     1501 cagctgacat cccagactac ctgaaactca gctttccgga aggattcaag tgggaacgcg
     1561 tcatgaattt cgaagatggc ggagtggtca ccgtgactca agacagctcg ttgcaagacg
     1621 gggagtttat ctacaaggtg aagctccggg gaactaactt cccatcagac ggtccggtca
     1681 tgcagaaaaa gacgatgggt tgggaagcct cctcggagag aatgtaccca gaggacggcg
     1741 cgctgaaggg tgaaatcaaa cagagactca aactgaaaga tggaggacac tacgacgcgg
     1801 aagtcaagac cacctacaag gcaaagaaac cagtgcagtt gccaggagcg tacaacgtga
     1861 acatcaaact tgatatcacc tcgcacaacg aagattacac cattgtggag caatacgaac
     1921 gggcggaagg aaggcattcg accgggggaa tggacgaatt atacaagggc tccgggagcg
     1981 gaatggtgag caagggagaa gaagataaca tggctattat caaggaattt atgcgcttca
     2041 aggttcatat ggagggctca gtgaacgggc acgaatttga aatcgaggga gagggagaag
     2101 gcagaccgta cgaaggcacc cagactgcta agctgaaggt tactaaggga ggacctctgc
     2161 ccttcgcatg ggacatcctt tcgccgcagt tcatgtacgg ttccaaggct tatgtgaagc
     2221 acccggcaga cattccggat tacctgaagc tgtcattccc tgagggcttt aagtgggagc
     2281 gcgtgatgaa ctttgaagat ggtggagtgg tgactgtgac ccaagactcc tccctgcaag
     2341 atggggagtt catctacaaa gtcaagttgc gcggaactaa tttcccttcg gacgggccgg
     2401 tgatgcagaa gaaaactatg ggatgggaag cttcatcgga aaggatgtac cccgaggacg
     2461 gagccctgaa aggggagatc aagcagcggc tcaagctcaa ggatggcggt cattacgacg
     2521 ccgaagtcaa gaccacgtac aaggccaaga agccggtgca gctccctggc gcgtacaatg
     2581 tcaatatcaa gctcgacatc acgtcccata atgaggatta tactatcgtg gaacagtacg
     2641 agcgcgccga aggacggcac tcaaccggag gaatggacga gctgtacaag agatctcgag
     2701 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat
     2761 aagtaatgat cataatcagc catatcacat ctgtagaggt tttacttgct ttaaaaaacc
     2821 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     2881 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2941 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     3001 tctggatcaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     3061 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     3121 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     3181 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3241 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3301 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3361 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3421 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3481 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3541 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3601 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3661 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3721 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3781 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3841 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3901 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3961 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4021 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4081 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4141 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4201 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4261 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4321 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4381 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4441 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4501 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4561 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4621 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4681 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4741 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4801 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4861 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4921 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4981 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5041 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5101 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5161 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     5221 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     5281 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     5341 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     5401 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     5461 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     5521 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     5581 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
     5641 caagttggga tctttgcatt ggcccacggc tctcaggatg gggatgctcc ccttcagcac
     5701 ccggttcccc ttggaaactg atggtcctgg ctctgtggca tggcagtggc actgtgagga
     5761 gccccctacc agcagcacac agtggggttg gcactgccac gctccggatg ccgcgctctg
     5821 atccaacccc ataatcaagg gaacccgaat tgccccatca ttgcccccac cacccccatc
     5881 ctgccgggcc ctcacacccc acgctgcctt ctggtgacat tccccagccc aaacccacgg
     5941 cttcatggct accgcggggc atttcccatt gccgccccat tatcagctct gcacacctcc
     6001 cgctgtaccc atgcctcgtg gctgcccttc tttgacgtat aatcttctaa ttaatacccg
     6061 gccttgtcaa agtggagcac aaacgttaat taattcccca gcaggcaggt aattaacagt
     6121 gtgactccct ttttgctgcg agtggggctg atacagagag atgtggcact atggagccca
     6181 cggggtcctg gcactgggtg cccacggagg tccccatgtg ctgcagtgtc accgcctccg
     6241 aggagacagt attgtccctg cggtgtccct gcagctcagc tctgtccaca gggccacctc
     6301 cagtttggag gggacacaat gcagccccga tgcaacccat cctcgcagca tcccagggac
     6361 aaagacccca ctgcaagacc gcacacaggg ctgggtcccg ctcccctaat atctacagtg
     6421 cttcttgcat ggccccttaa tcaatgcagt taatcagcat gcgctcatgc accgctctgg
     6481 agctgcaaag cccctcgcag cgctgctcac caacaccgcg caccgccccg gcccagcctg
     6541 cagcacgcgc tgcaaacagg aaagaaacaa aatattgccc aaatgtaggc aaaggcattc
     6601 ggctgccttg acctccgccg ggccgggccc tgcctgactc agctccttac tcagcgctcg
     6661 cttcctccct ccggctgcca ccgccgcagc gcacaccctg acaaagagtg gcccttaacg
     6721 ggctctgagg tgcacccagc agtgcactca gcagtccaag ggccggcctg gaggtttgca
     6781 ccgctacgtg ctgacattag cattgaactt ggccctgggt agtgctgcag gccgggcggg
     6841 gtgggtgtag agagtgcagc gcgcgttgca cccggtgccc cttcccctcc cttgcatccc
     6901 agcaggctgc accccagcac caggcccgtg catgcatgct cctggtgtta ttgcagcctg
     6961 gtgcatgcat gcgtcttgat ggtgcagcgc tgtgcatgca tcctccttgg tgtgtagcag
     7021 tctagtgcat gcatacccct cggtgttatt gctgctctgt gcacgcacgc tcattgtatc
     7081 acttcatccc agtgcatgca ctcacactgg agcgattgct gctcggtgca cgcacactca
     7141 ttgtatcact gcagctcagt ggctgcacgc acaccggtgt tattgctgct cggtgcgtgc
     7201 atgcacatca gtgtcgctgc agctcagtgc atgcacgctc attgcccatc gctatccctg
     7261 cctctcctgc tggcgctccc cgggaggtga cttcaagggg accgcaggac cacctcgggg
     7321 gtggggggag ggctgcacac gcggaccccg ctccccctcc ccaacaaagc actgtggaat
     7381 caaaaagggg ggagggggga tggaggggcg cgtcacaccc ccgccccaca ccctcacctc
     7441 gaggtgagcc ccacgttctg cttcactctc cccatctc
//
