LOCUS       sequence-391811-        7961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of the centrosome targeting carboxy-terminal domain (CTD)
            of Cdk5rap2 fused to tandem (td)-mCherry. Used to displace 
            endogenous Cdk5rap2 from centrosomes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7961)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 7961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pBactin-td-mCherry-Cdk5rap2-CTD
FEATURES             Location/Qualifiers
     source          1..7961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1179..1201
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      1254..1263
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3357..3491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3394..3413)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3448..3467
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3505..3521)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3505..3521)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3518..3540)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3529..3545
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3553..3559,3560..3577,3578..3583))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3598..3619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3736..3753)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3907..4495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3987..4006)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4666..5457,5458..5526))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5289..5308
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5527..5631)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5699..5717
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5755..5777)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5877..5896
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6090..6112
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6104..6121
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6105..6121
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(7922..7961,1..236)
                     /label=chicken beta-actin promoter
ORIGIN
        1 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
       61 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgrg gggcggggcg
      121 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      181 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      241 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      301 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      361 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      421 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      481 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      541 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
      601 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
      661 gkgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
      721 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
      781 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
      841 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
      901 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
      961 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1021 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1081 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgtccgca
     1141 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1201 cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagcta gcagccacca
     1261 tggtcagcaa aggggaagag gataacatgg ccatcatcaa agagttcatg cggttcaagg
     1321 tgcacatgga aggcagcgtg aacggacatg agttcgagat cgaaggagaa ggagagggac
     1381 gcccttacga gggaactcag accgcaaagc tgaaagtgac taagggtggc cctctgccgt
     1441 ttgcgtggga cattctgtcc ccgcaattca tgtatggctc aaaagcctac gtcaaacacc
     1501 cagctgacat cccagactac ctgaaactca gctttccgga aggattcaag tgggaacgcg
     1561 tcatgaattt cgaagatggc ggagtggtca ccgtgactca agacagctcg ttgcaagacg
     1621 gggagtttat ctacaaggtg aagctccggg gaactaactt cccatcagac ggtccggtca
     1681 tgcagaaaaa gacgatgggt tgggaagcct cctcggagag aatgtaccca gaggacggcg
     1741 cgctgaaggg tgaaatcaaa cagagactca aactgaaaga tggaggacac tacgacgcgg
     1801 aagtcaagac cacctacaag gcaaagaaac cagtgcagtt gccaggagcg tacaacgtga
     1861 acatcaaact tgatatcacc tcgcacaacg aagattacac cattgtggag caatacgaac
     1921 gggcggaagg aaggcattcg accgggggaa tggacgaatt atacaagggc tccgggagcg
     1981 gaatggtgag caagggagaa gaagataaca tggctattat caaggaattt atgcgcttca
     2041 aggttcatat ggagggctca gtgaacgggc acgaatttga aatcgaggga gagggagaag
     2101 gcagaccgta cgaaggcacc cagactgcta agctgaaggt tactaaggga ggacctctgc
     2161 ccttcgcatg ggacatcctt tcgccgcagt tcatgtacgg ttccaaggct tatgtgaagc
     2221 acccggcaga cattccggat tacctgaagc tgtcattccc tgagggcttt aagtgggagc
     2281 gcgtgatgaa ctttgaagat ggtggagtgg tgactgtgac ccaagactcc tccctgcaag
     2341 atggggagtt catctacaaa gtcaagttgc gcggaactaa tttcccttcg gacgggccgg
     2401 tgatgcagaa gaaaactatg ggatgggaag cttcatcgga aaggatgtac cccgaggacg
     2461 gagccctgaa aggggagatc aagcagcggc tcaagctcaa ggatggcggt cattacgacg
     2521 ccgaagtcaa gaccacgtac aaggccaaga agccggtgca gctccctggc gcgtacaatg
     2581 tcaatatcaa gctcgacatc acgtcccata atgaggatta tactatcgtg gaacagtacg
     2641 agcgcgccga aggacggcac tcaaccggag gaatggacga gctgtacaag agatctcaca
     2701 tgctgtgcct gattgaggac tatgatgccc tctataagca gatcagctgg ggccagacac
     2761 tgcttgccaa gatggatatt caaacccaag aggctctgag ccccacaagt cagaagctgg
     2821 gaccaaaggc ttcattctct gtgcctctga gcaagtttct ctccagcatg aacacagcca
     2881 agctgatcct ggaaaaagcc tccaggttgc tgaagctctt ctggagggtc tctgtcccca
     2941 ccaacggcca gtgttccctt cactgtgacc agattggaga aatgaaggca gagatcacca
     3001 aactacacaa aaaattgttt gaacaagaaa agaagctgca gaacacagca aaacttctgc
     3061 agcagagcaa gcaccaggag aaaatcatct ttgatcagtt ggtcatcacc caccaagtcc
     3121 ttcggaaagc caggggaaac ctggagctca ggcctagagc tgcccatcca ggaacatcca
     3181 gtcccagcag accgggctca tgagtcgacg gtaccgcggg cccgggatcc accggatcta
     3241 gataagtaat gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa
     3301 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     3361 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3421 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     3481 atgtctggat caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     3541 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3601 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3661 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3721 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3781 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3841 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3901 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3961 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4021 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4081 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4141 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4201 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4261 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4321 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4381 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4441 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4501 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4561 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4621 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4681 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4741 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4801 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4861 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4921 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4981 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     5041 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     5101 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5161 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5221 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5281 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5341 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5401 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5461 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5521 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5581 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5641 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     5701 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     5761 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     5821 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     5881 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     5941 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     6001 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     6061 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     6121 tgccaagttg ggatctttgc attggcccac ggctctcagg atggggatgc tccccttcag
     6181 cacccggttc cccttggaaa ctgatggtcc tggctctgtg gcatggcagt ggcactgtga
     6241 ggagccccct accagcagca cacagtgggg ttggcactgc cacgctccgg atgccgcgct
     6301 ctgatccaac cccataatca agggaacccg aattgcccca tcattgcccc caccaccccc
     6361 atcctgccgg gccctcacac cccacgctgc cttctggtga cattccccag cccaaaccca
     6421 cggcttcatg gctaccgcgg ggcatttccc attgccgccc cattatcagc tctgcacacc
     6481 tcccgctgta cccatgcctc gtggctgccc ttctttgacg tataatcttc taattaatac
     6541 ccggccttgt caaagtggag cacaaacgtt aattaattcc ccagcaggca ggtaattaac
     6601 agtgtgactc cctttttgct gcgagtgggg ctgatacaga gagatgtggc actatggagc
     6661 ccacggggtc ctggcactgg gtgcccacgg aggtccccat gtgctgcagt gtcaccgcct
     6721 ccgaggagac agtattgtcc ctgcggtgtc cctgcagctc agctctgtcc acagggccac
     6781 ctccagtttg gaggggacac aatgcagccc cgatgcaacc catcctcgca gcatcccagg
     6841 gacaaagacc ccactgcaag accgcacaca gggctgggtc ccgctcccct aatatctaca
     6901 gtgcttcttg catggcccct taatcaatgc agttaatcag catgcgctca tgcaccgctc
     6961 tggagctgca aagcccctcg cagcgctgct caccaacacc gcgcaccgcc ccggcccagc
     7021 ctgcagcacg cgctgcaaac aggaaagaaa caaaatattg cccaaatgta ggcaaaggca
     7081 ttcggctgcc ttgacctccg ccgggccggg ccctgcctga ctcagctcct tactcagcgc
     7141 tcgcttcctc cctccggctg ccaccgccgc agcgcacacc ctgacaaaga gtggccctta
     7201 acgggctctg aggtgcaccc agcagtgcac tcagcagtcc aagggccggc ctggaggttt
     7261 gcaccgctac gtgctgacat tagcattgaa cttggccctg ggtagtgctg caggccgggc
     7321 ggggtgggtg tagagagtgc agcgcgcgtt gcacccggtg ccccttcccc tcccttgcat
     7381 cccagcaggc tgcaccccag caccaggccc gtgcatgcat gctcctggtg ttattgcagc
     7441 ctggtgcatg catgcgtctt gatggtgcag cgctgtgcat gcatcctcct tggtgtgtag
     7501 cagtctagtg catgcatacc cctcggtgtt attgctgctc tgtgcacgca cgctcattgt
     7561 atcacttcat cccagtgcat gcactcacac tggagcgatt gctgctcggt gcacgcacac
     7621 tcattgtatc actgcagctc agtggctgca cgcacaccgg tgttattgct gctcggtgcg
     7681 tgcatgcaca tcagtgtcgc tgcagctcag tgcatgcacg ctcattgccc atcgctatcc
     7741 ctgcctctcc tgctggcgct ccccgggagg tgacttcaag gggaccgcag gaccacctcg
     7801 ggggtggggg gagggctgca cacgcggacc ccgctccccc tccccaacaa agcactgtgg
     7861 aatcaaaaag gggggagggg ggatggaggg gcgcgtcaca cccccgcccc acaccctcac
     7921 ctcgaggtga gccccacgtt ctgcttcact ctccccatct c
//
