LOCUS       sequence-391828-       12971 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Cyclin dependent kinase 5 regulatory 
            subunit-associated protein 2 (Cdk5rap2) fused to tandem 
            (td)-mCherry. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12971)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 12971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pBactin-td-mCherry-Cdk5rap2
FEATURES             Location/Qualifiers
     source          1..12971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1179..1201
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      1254..1263
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             6508..6516
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    8367..8501
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8404..8423)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8458..8477
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8515..8531)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8515..8531)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8528..8550)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8539..8555
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8563..8569,8570..8587,8588..8593))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8608..8629
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8746..8763)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8917..9505)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8997..9016)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9676..10467,10468..10536))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10299..10318
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10537..10641)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10709..10727
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10765..10787)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10887..10906
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11100..11122
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11114..11131
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11115..11131
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(12932..12971,1..236)
                     /label=chicken beta-actin promoter
ORIGIN
        1 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
       61 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
      121 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      181 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      241 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      301 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      361 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      421 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      481 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      541 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
      601 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
      661 gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
      721 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
      781 cgcggggctc gccgtgccgg gcggggggtg gcggcaggkg ggggtgccgg gcggggcggg
      841 gccgcctcgg gccggggrgg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
      901 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
      961 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1021 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1081 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgtccgca
     1141 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1201 cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagcta gcagccacca
     1261 tggtcagcaa aggggaagag gataacatgg ccatcatcaa agagttcatg cggttcaagg
     1321 tgcacatgga aggcagcgtg aacggacatg agttcgagat cgaaggagaa ggagagggac
     1381 gcccttacga gggaactcag accgcaaagc tgaaagtgac taagggtggc cctctgccgt
     1441 ttgcgtggga cattctgtcc ccgcaattca tgtatggctc aaaagcctac gtcaaacacc
     1501 cagctgacat cccagactac ctgaaactca gctttccgga aggattcaag tgggaacgcg
     1561 tcatgaattt cgaagatggc ggagtggtca ccgtgactca agacagctcg ttgcaagacg
     1621 gggagtttat ctacaaggtg aagctccggg gaactaactt cccatcagac ggtccggtca
     1681 tgcagaaaaa gacgatgggt tgggaagcct cctcggagag aatgtaccca gaggacggcg
     1741 cgctgaaggg tgaaatcaaa cagagactca aactgaaaga tggaggacac tacgacgcgg
     1801 aagtcaagac cacctacaag gcaaagaaac cagtgcagtt gccaggagcg tacaacgtga
     1861 acatcaaact tgatatcacc tcgcacaacg aagattacac cattgtggag caatacgaac
     1921 gggcggaagg aaggcattcg accgggggaa tggacgaatt atacaagggc tccgggagcg
     1981 gaatggtgag caagggagaa gaagataaca tggctattat caaggaattt atgcgcttca
     2041 aggttcatat ggagggctca gtgaacgggc acgaatttga aatcgaggga gagggagaag
     2101 gcagaccgta cgaaggcacc cagactgcta agctgaaggt tactaaggga ggacctctgc
     2161 ccttcgcatg ggacatcctt tcgccgcagt tcatgtacgg ttccaaggct tatgtgaagc
     2221 acccggcaga cattccggat tacctgaagc tgtcattccc tgagggcttt aagtgggagc
     2281 gcgtgatgaa ctttgaagat ggtggagtgg tgactgtgac ccaagactcc tccctgcaag
     2341 atggggagtt catctacaaa gtcaagttgc gcggaactaa tttcccttcg gacgggccgg
     2401 tgatgcagaa gaaaactatg ggatgggaag cttcatcgga aaggatgtac cccgaggacg
     2461 gagccctgaa aggggagatc aagcagcggc tcaagctcaa ggatggcggt cattacgacg
     2521 ccgaagtcaa gaccacgtac aaggccaaga agccggtgca gctccctggc gcgtacaatg
     2581 tcaatatcaa gctcgacatc acgtcccata atgaggatta tactatcgtg gaacagtacg
     2641 agcgcgccga aggacggcac tcaaccggag gaatggacga gctgtacaag agatctatga
     2701 tggactcagg gatggaagag gacgtcaccc tgcctgggac cctcagcggc tgcagtggcc
     2761 ttcaccctgt tctaccaagt gatctggatg tcatcagtga cactactggc ttgggaaatg
     2821 gagtgctccc aatcatgtca gaagaaaagg tctctcccac cagagcccgg aacatgaagg
     2881 actttgaaaa ccaaatcact gagctgaaga aggagaactt caatctaaag ctccggatct
     2941 acttccttga ggagaggata cagcaggagt ttgctggccc cacagagcac atctacaaga
     3001 agaacattga actcaaggtg gaagtggaaa gtctgaagcg tgagctccag gagagagatc
     3061 agttgctcgt caaagcctcc aaagccgtgg agagcctagc agaagggggt ggttctgaaa
     3121 tccagcgggt gaaagaagat gcaaggaaga aggtgcagca ggtggaagaa ctcctaacta
     3181 aaaggataca tcttctagaa gaagatgtga aagccgccca agcagaactg gagaaggcct
     3241 ttgcagggac agaaacagag aaggctcttc ggctcagctt agaaagcaag ctctcggcga
     3301 tgaagaagat gcaggagggg gacctggaga tgactctggc tctggaagag aaggacagac
     3361 tgattgagga gttgaagctg tctttgaaga gcaaagaagc tttaattcag tgccttaaag
     3421 aggaaaaatc tcagatggca tctcctgacg agaacgtgtc atctggagaa ctccgaggac
     3481 tttctgccac tctaagagaa gaaaaagaga gagacgctga ggaacggcag aaggagagaa
     3541 accactttga agagaggatc caggcacttc aggaggacct gagagagaag gaaagagaaa
     3601 ttgctacaga gaagaaaaat agcttaaaga gggataaagc cattcagggt ctaaccatgg
     3661 cattaaaatc aaaggaaaaa gaggagtctg agaactgtga ggctgcgctg gcagagaagg
     3721 aagccctgtt ggccaagctg cactcggaaa atgtcacaaa gaatactgag aaccataggt
     3781 tgctcaggaa tgttaaaaag gtcacccagg agttgaatga cctgaagaag gagaagctga
     3841 gactagagcg ggacctggag gaagcccacc gagaagggaa cagaggagcc cgaaccatcc
     3901 atgaccttag aaatgaagtt gagaaactac gcaaggaagt gtgtgagaga gagaaggctg
     3961 tggagaagca ttacaagagt cttccaggcg aaagcagttc aaagttccat tctcaagaac
     4021 aagtggtcaa aggtctgaca gaaagtgcca gtcaggagga cctgctgctg cagaaatcca
     4081 atgaaaaaga tttggaagca atacagcaga actgttattt aatgactgca gaggagctca
     4141 agtttgggag cgatggctta ataacagaaa agtgttctca gcagtcacca gacagcaaac
     4201 tcatcttctc caaggaaaag caacaatcag agtatgaagg gctgactggg gacttaaaga
     4261 cagaacagaa cgtctatgcc catctggcaa agaatctgca ggacacagac agcaaactgc
     4321 aggctgaatt gaaaagggtt cttgccctcc ggaagcagct ggagcaggat gtgcttgcct
     4381 atcggaatct gcagacggcc ctgcaggagc agctcagtga aattcggaag cgggaagaag
     4441 agccgttttc attttatagc gatcaaacat catatctaag tatttgcctt gaagagcaca
     4501 gtcagtttca attggagcat ttttctcaag aagaaatcaa gaaaaaggtc attgacctca
     4561 tacagcttgt gaaggacctg catgcagaca accagcacct gaagaaaacc atttttgata
     4621 tctcttgcat gggtgtccag ggaaatgaca gacttgagtc aacaaaacaa gcagagatca
     4681 tggaggacta tgctgagggt ggcggcaagg atggatatgt aaggcacatg gattccaata
     4741 ttttagacca tgatggggcc cacacacctg atacctctga agaccatagc ctagaggatg
     4801 agctcctgag cctactcgcc actttcttca gtaagaaggc tacaccgtcg ctggagtcca
     4861 ggccggatct gctgaaagca cttggtgccc tgcttttgga acggatatgc ttggcagaac
     4921 agggaagtcc tggagatcac tctgatagta aagctgagaa ggcacttgag caagtagctg
     4981 ttcggttgag agatgaactt ggccattcct gcctagccaa ctcattctcc aagtcacaca
     5041 gtgagctcaa gtcaccgcgg ggcacctggc tagtaaagac aggtgatgaa gccaaggtgg
     5101 agctgaagag tgtctcagtg cagaccatga ccatagagga cagcacctgt ggattcaaac
     5161 ctgagaggaa gagagaagct tgggcaggga aaccagaaga ggccgttttc agcactgagc
     5221 ttgagtcaga ggcgttgggt gagatgccag ggctacaggc cactcacctc tcattcccca
     5281 gtgccatcga caaggatgac cagaagacag gcttgctgat ccaattaaaa acacctgagt
     5341 tattggaaaa tttgtataat cttcctgcct cccaagaggt ggtagctcaa ctccagggcc
     5401 aggttctaga gctgcagaaa gagctgaaag agtataagat ccgcaacaag caactccttg
     5461 ataagctaat tctggcagag gcaatgatgg aggggatggc agtgcccaac tctgctccgg
     5521 taaacgtgcc tgcagcccag gctgttgtga gaacagcctt ccaaggcaag ccaggcgagc
     5581 aagaaggaca tgaaaccaca cattctgccg gaagagacaa ggaagtagac agtgaccaat
     5641 atacaagctt tgagattgat tctgaaatct gtccacctga tgaccttgcc ttattaccag
     5701 catgcaaaga gaaccttgaa gacttcttag gcccaccctc catagccact tacctggatt
     5761 ccaagagtca gctctctgtt aaagtcagtg tggttggaac tgaccagtct gagaatatca
     5821 atctccctga tgatacagaa gccttaaaac agaaaatcca tgacttgcaa actgagctgg
     5881 aaggctaccg gaacatcatt gtccagctac agaagcattc tcagtgcagt gaggctatta
     5941 ttacagtttt gtgtgggaca gaaggggccc aagatggcct gaacaagcct aagggccaca
     6001 tcgatgaaga agagatgaca ttttcaagtt tgcaccaagt aagatatgtg aagcacatga
     6061 aaatcctccg tccactaacc ccagaaataa ttgatggcaa gatgctagag agcctcaagc
     6121 agcagctggt agaacaggag caggagctac agaaggagca ggatttgaac ttggaacttt
     6181 tcggtgaaat ccataacctg cagaacaagt tccgagacct ctcgccatca agatacgatt
     6241 cattggttca gtcccaagca agggagctct ctcttcagcg gcagcaaatt aaggatagtc
     6301 atgatatctg tgtcgtttgc catcaacaca tgagcaccat gatcaaggca tttgaggaac
     6361 ttctgcaggc cagtgatgtg gacagttgtg tggctgaggg cttccgcgag cagctaacac
     6421 agtgtgctgg actgctagag caactggaga agctgtttct ccatggaaag tcagccagag
     6481 tggaaccaca tacccagact gaactgctaa ggaggttaag aactgaagaa gacaacttac
     6541 cttaccaaca tctcctgcct gaatctcctg agccttctgc ctctcatgct ctgtcagatg
     6601 atgaaatgtc tgagaagtcc ttcctgtcca gagaaccgaa gccagacagt gagactgaga
     6661 agtacccaac aatagcgagc cgttttcctc aagacttact aatggaacac atacaggaaa
     6721 ttcgaacttt gagaaagcat ttggaagaat ctattaaaac aaatgagaag ctccggaaac
     6781 agctggaacg gcagggatgt gagactgacc aaggctctac aaatgtttct gcttacagct
     6841 cagaactgca caattctctg acatcagaaa tacagttcct gaggaagcag aatgaggccc
     6901 tcagtacgat gctcgagaag gggtccaaag aaaaacagaa agagaatgaa aaattgcgtg
     6961 agtccctggc caggaagact gagagtcttg agcaccttca gctagagtat gccagtgtaa
     7021 gggaagaaaa tgaaagactg cggagagaca tcagtgagaa ggagagacaa aaccagcagt
     7081 tgactcagga ggtctgcagc agcctccaag agctgagcag ggtacaggaa gaggcaaagt
     7141 caagacagca gctgctctta cagaaagacg agctgcttca gtccctccaa atggagctta
     7201 aggtgtatga gaagctggcc gaggagcatc agaagctaca gcaagaggcc cgaggtgagg
     7261 catgtggagg aggccagaag gggcaggatc ctttcagcaa cctgcatggt ctcctgaaag
     7321 agatacaggt tctccgagac caggcagaaa ggagcattca aactaacaac acactgaaga
     7381 gcaagctgga gaagcagcta tcacaggggt caaagcaggc ccaggaaggg gccctcactt
     7441 tggctgttca ggccctgtct gtcactgagt ggtcccttca gttggacaaa cacgatgtca
     7501 acaaatgtcc tgaggccagc gataattcat ttgatctatt tgaatctacc caggcaatgg
     7561 cacctaaatc agcttctgag actcctgtcc tttctggaac tgatgttgat tccctctcct
     7621 gtgacagtac cagttctgcc accagcccct cttgcatgcc ctgcctggtt gctggccgcc
     7681 acctgtgggc cagcaagagt ggccaccaca tgctgtgcct gattgaggac tatgatgccc
     7741 tctataagca gatcagctgg ggccagacac tgcttgccaa gatggatatt caaacccaag
     7801 aggctctgag ccccacaagt cagaagctgg gaccaaaggc ttcattctct gtgcctctga
     7861 gcaagtttct ctccagcatg aacacagcca agctgatcct ggaaaaagcc tccaggttgc
     7921 tgaagctctt ctggagggtc tctgtcccca ccaacggcca gtgttccctt cactgtgacc
     7981 agattggaga aatgaaggca gagatcacca aactacacaa aaaattgttt gaacaagaaa
     8041 agaagctgca gaacacagca aaacttctgc agcagagcaa gcaccaggag aaaatcatct
     8101 ttgatcagtt ggtcatcacc caccaagtcc ttcggaaagc caggggaaac ctggagctca
     8161 ggcctagagc tgcccatcca ggaacatcca gtcccagcag accgggctca tgagtcgacg
     8221 gtaccgcggg cccgggatcc accggatcta gataagtaat gatcataatc agccatatca
     8281 catctgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     8341 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     8401 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8461 gtttgtccaa actcatcaat gtatcttatc atgtctggat caattcgtaa tcatgtcata
     8521 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8581 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8641 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8701 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8761 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8821 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8881 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8941 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9001 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9061 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9121 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9181 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9241 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9301 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9361 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9421 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9481 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9541 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9601 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9661 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9721 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9781 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9841 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9901 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9961 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10021 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10081 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10141 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10201 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10261 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10321 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10381 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10441 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10501 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10561 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10621 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    10681 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    10741 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    10801 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    10861 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    10921 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    10981 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    11041 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    11101 ccagtcacga cgttgtaaaa cgacggccag tgccaagttg ggatctttgc attggcccac
    11161 ggctctcagg atggggatgc tccccttcag cacccggttc cccttggaaa ctgatggtcc
    11221 tggctctgtg gcatggcagt ggcactgtga ggagccccct accagcagca cacagtgggg
    11281 ttggcactgc cacgctccgg atgccgcgct ctgatccaac cccataatca agggaacccg
    11341 aattgcccca tcattgcccc caccaccccc atcctgccgg gccctcacac cccacgctgc
    11401 cttctggtga cattccccag cccaaaccca cggcttcatg gctaccgcgg ggcatttccc
    11461 attgccgccc cattatcagc tctgcacacc tcccgctgta cccatgcctc gtggctgccc
    11521 ttctttgacg tataatcttc taattaatac ccggccttgt caaagtggag cacaaacgtt
    11581 aattaattcc ccagcaggca ggtaattaac agtgtgactc cctttttgct gcgagtgggg
    11641 ctgatacaga gagatgtggc actatggagc ccacggggtc ctggcactgg gtgcccacgg
    11701 aggtccccat gtgctgcagt gtcaccgcct ccgaggagac agtattgtcc ctgcggtgtc
    11761 cctgcagctc agctctgtcc acagggccac ctccagtttg gaggggacac aatgcagccc
    11821 cgatgcaacc catcctcgca gcatcccagg gacaaagacc ccactgcaag accgcacaca
    11881 gggctgggtc ccgctcccct aatatctaca gtgcttcttg catggcccct taatcaatgc
    11941 agttaatcag catgcgctca tgcaccgctc tggagctgca aagcccctcg cagcgctgct
    12001 caccaacacc gcgcaccgcc ccggcccagc ctgcagcacg cgctgcaaac aggaaagaaa
    12061 caaaatattg cccaaatgta ggcaaaggca ttcggctgcc ttgacctccg ccgggccggg
    12121 ccctgcctga ctcagctcct tactcagcgc tcgcttcctc cctccggctg ccaccgccgc
    12181 agcgcacacc ctgacaaaga gtggccctta acgggctctg aggtgcaccc agcagtgcac
    12241 tcagcagtcc aagggccggc ctggaggttt gcaccgctac gtgctgacat tagcattgaa
    12301 cttggccctg ggtagtgctg caggccgggc ggggtgggtg tagagagtgc agcgcgcgtt
    12361 gcacccggtg ccccttcccc tcccttgcat cccagcaggc tgcaccccag caccaggccc
    12421 gtgcatgcat gctcctggtg ttattgcagc ctggtgcatg catgcgtctt gatggtgcag
    12481 cgctgtgcat gcatcctcct tggtgtgtag cagtctagtg catgcatacc cctcggtgtt
    12541 attgctgctc tgtgcacgca cgctcattgt atcacttcat cccagtgcat gcactcacac
    12601 tggagcgatt gctgctcggt gcacgcacac tcattgtatc actgcagctc agtggctgca
    12661 cgcacaccgg tgttattgct gctcggtgcg tgcatgcaca tcagtgtcgc tgcagctcag
    12721 tgcatgcacg ctcattgccc atcgctatcc ctgcctctcc tgctggcgct ccccgggagg
    12781 tgacttcaag gggaccgcag gaccacctcg ggggtggggg gagggctgca cacgcggacc
    12841 ccgctccccc tccccaacaa agcactgtgg aatcaaaaag gggggagggg ggatggaggg
    12901 gcgcgtcaca cccccgcccc acaccctcac ctcgaggtga gccccacgtt ctgcttcact
    12961 ctccccatct c
//
