LOCUS       sequence-391829-        8813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of neural precursor cell expressed developmentally 
            down-regulated 1 (Nedd1) fused to mOrange2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8813)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 8813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pBactin-Nedd1-mOrange2
FEATURES             Location/Qualifiers
     source          1..8813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1179..1201
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             3303..4013
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    4209..4343
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4246..4265)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4300..4319
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4357..4373)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4357..4373)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4370..4392)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4381..4397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4405..4411,4412..4429,4430..4435))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4450..4471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4588..4605)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4759..5347)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4839..4858)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5518..6309,6310..6378))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6141..6160
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6379..6483)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6551..6569
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6607..6629)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6729..6748
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6942..6964
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6956..6973
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6957..6973
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(8774..8813,1..236)
                     /label=chicken beta-actin promoter
ORIGIN
        1 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
       61 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
      121 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      181 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      241 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      301 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      361 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      421 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      481 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      541 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
      601 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
      661 gkgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
      721 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
      781 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
      841 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
      901 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
      961 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1021 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1081 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgtccgca
     1141 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1201 cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagcta gcagccacca
     1261 tgcaggaaaa cctcagattt gcctcatcag gagatgatgt taaaatatgg gatgcttcgt
     1321 ttctgacatt ggtggataaa ttcaaccccc acacgtcacc gcatggaatc agttcaatct
     1381 gttggagcag caataataac ttcctagtga ctgcgtcttc tggtggtgac aaaatagtgg
     1441 tgtcaagttg caaatgtaag cccgttccac ttttggagct tgcagaaggg caaaagcaga
     1501 catgtgtcga tttaaattct acatcaatgt atttggcaag tggaggccta aataacactg
     1561 ttaatatttg ggatttaaag tcaaaacgac ttcatcggtc tcttaaggac cataaacggg
     1621 aagtaacttg tgtggcgtac aattggaatg attgttacat tgcttccggg tctctcagtg
     1681 gtgagattat cttacacagc gtaaccacta atacctccag tacgcctttc ggccatggga
     1741 gtaagcagcc tgtccggcac atcaagtact cgttgtttag gaagtctctc ttgggcagcg
     1801 tttcggataa tggagtagtg accctctggg atgtgaacag tcagagtgca taccacagct
     1861 ttgacagtac acacaaagcc ccagcttcag gcctctgctt ctctcctgtc aacgaactgc
     1921 tgtttgtgac cataggcctg gataaaagaa tcatcctgta tgacacttca agtaagaagc
     1981 tggtgaaaac tttagtggct gacactcctc tgactgctgt agattttatg ccagatggag
     2041 ccagtttggc tattggatcc tcccgtggga aaatatatca gtatgactta aggatgctga
     2101 aatctcccgt gaaaaccatc agcgcccaca agacatctgt gcagtgtata gcatttcagt
     2161 actccacatc tctgacgaag gcaagtttaa ataaaggctg ctcaaataag gccacggcag
     2221 cgaataaacg gagtgttcct gtgggcagca gcagtggggc tgcccagaat tctggaattg
     2281 tcagagaagc acccagccct tccattgcca cagttctccc acagcccgtg acgacagctg
     2341 tgggcaaagg agcaggggct gctcaggacg aagcaggttt gcctcgaagc aaaaatacag
     2401 atatcttatc taaggaaact gatgctggaa aaagtcaaga cttctccagc tttgatgaca
     2461 cggggaagag tagtttaggg gacatgtttt caccaattag agatgatgct gtagtcaaca
     2521 aaggaggtga cgagtctgta ggcaaaggag atggccttga ctttctacca cagctaaact
     2581 cggtgttccc cctgaggaag aatgcaggct cctccagcac tctggtgctg cactccagcc
     2641 cttttaatgt ccttatgggg acagggaagg aagagcatga gagtcacgag ctgtcagccg
     2701 agtctaagaa ggcacacctt gggaagccgg agcctaagga ctccttgaag cagtttacaa
     2761 agttgatctc ctctggtgct gaacctggaa ttctaaacac ctgtccatca tctaaccaag
     2821 caagaaatct tgaaaagttt gaaaagccag aaaaagatat tgaagtccag ttgatgcacg
     2881 aaccctcagt caatggaccc tcaaccccag taccaaaggc agcctcctcc gtcactgctg
     2941 gagtagccag ttcactctca gaaaaaatag tcgacaccct tgggaacagt cggccggccg
     3001 cgccacttac ctctgtccag atccgcttca ttcagaacat gatacaagaa accctggatg
     3061 acttcaggga agcttgccat agggacattg tgaatctgca agtggaaatg attaaacagt
     3121 ttcatatgca actgaatgaa atgcactctt tgctggaaag atactcagtg aatgaaggct
     3181 tagtggctga gatcgaaagg ctgcgagaag aaaacaaaag gctgcgggcc cacttctcaa
     3241 gcctcctcga cggcagcaag cttggtggag gcagcgcatc aggcggaagc ggaagcagat
     3301 ctatggtgag caagggcgag gagaataaca tggccatcat caaggagttc atgcgcttca
     3361 aggtgcgcat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     3421 gccgccccta cgagggcttt cagaccgcta agctgaaggt gaccaagggt ggccccctgc
     3481 ccttcgcctg ggacatcctg tcccctcatt tcacctacgg ctccaaggcc tacgtgaagc
     3541 accccgccga catccccgac tacttcaagc tgtccttccc cgagggcttc aagtgggagc
     3601 gcgtgatgaa ctacgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     3661 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     3721 tgatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     3781 gtgccctgaa gggcaagatc aagatgaggc tgaagctgaa ggacggcggc cactacacct
     3841 ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacatcg
     3901 tcgacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     3961 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag taagcggccg
     4021 ccatatgcat cctaggccta ttaatattcc ggagtatacg tagccggcta acgttaacaa
     4081 ccggtacctc tagataagta atgatcataa tcagccatat cacatctgta gaggttttac
     4141 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     4201 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4261 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4321 atgtatctta tcatgtctgg atcaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     4381 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     4441 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     4501 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     4561 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4621 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4681 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4741 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4801 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4861 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4921 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4981 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5041 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5101 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5161 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     5221 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5281 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5341 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5401 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     5461 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     5521 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     5581 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     5641 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5701 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5761 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5821 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5881 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     5941 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     6001 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     6061 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     6121 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     6181 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     6241 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     6301 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     6361 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     6421 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     6481 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     6541 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     6601 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     6661 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     6721 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     6781 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     6841 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     6901 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     6961 aacgacggcc agtgccaagt tgggatcttt gcattggccc acggctctca ggatggggat
     7021 gctccccttc agcacccggt tccccttgga aactgatggt cctggctctg tggcatggca
     7081 gtggcactgt gaggagcccc ctaccagcag cacacagtgg ggttggcact gccacgctcc
     7141 ggatgccgcg ctctgatcca accccataat caagggaacc cgaattgccc catcattgcc
     7201 cccaccaccc ccatcctgcc gggccctcac accccacgct gccttctggt gacattcccc
     7261 agcccaaacc cacggcttca tggctaccgc ggggcatttc ccattgccgc cccattatca
     7321 gctctgcaca cctcccgctg tacccatgcc tcgtggctgc ccttctttga cgtataatct
     7381 tctaattaat acccggcctt gtcaaagtgg agcacaaacg ttaattaatt ccccagcagg
     7441 caggtaatta acagtgtgac tccctttttg ctgcgagtgg ggctgataca gagagatgtg
     7501 gcactatgga gcccacgggg tcctggcact gggtgcccac ggaggtcccc atgtgctgca
     7561 gtgtcaccgc ctccgaggag acagtattgt ccctgcggtg tccctgcagc tcagctctgt
     7621 ccacagggcc acctccagtt tggaggggac acaatgcagc cccgatgcaa cccatcctcg
     7681 cagcatccca gggacaaaga ccccactgca agaccgcaca cagggctggg tcccgctccc
     7741 ctaatatcta cagtgcttct tgcatggccc cttaatcaat gcagttaatc agcatgcgct
     7801 catgcaccgc tctggagctg caaagcccct cgcagcgctg ctcaccaaca ccgcgcaccg
     7861 ccccggccca gcctgcagca cgcgctgcaa acaggaaaga aacaaaatat tgcccaaatg
     7921 taggcaaagg cattcggctg ccttgacctc cgccgggccg ggccctgcct gactcagctc
     7981 cttactcagc gctcgcttcc tccctccggc tgccaccgcc gcagcgcaca ccctgacaaa
     8041 gagtggccct taacgggctc tgaggtgcac ccagcagtgc actcagcagt ccaagggccg
     8101 gcctggaggt ttgcaccgct acgtgctgac attagcattg aacttggccc tgggtagtgc
     8161 tgcaggccgg gcggggtggg tgtagagagt gcagcgcgcg ttgcacccgg tgccccttcc
     8221 cctcccttgc atcccagcag gctgcacccc agcaccaggc ccgtgcatgc atgctcctgg
     8281 tgttattgca gcctggtgca tgcatgcgtc ttgatggtgc agcgctgtgc atgcatcctc
     8341 cttggtgtgt agcagtctag tgcatgcata cccctcggtg ttattgctgc tctgtgcacg
     8401 cacgctcatt gtatcacttc atcccagtgc atgcactcac actggagcga ttgctgctcg
     8461 gtgcacgcac actcattgta tcactgcagc tcagtggctg cacgcacacc ggtgttattg
     8521 ctgctcggtg cgtgcatgca catcagtgtc gctgcagctc agtgcatgca cgctcattgc
     8581 ccatcgctat ccctgcctct cctgctggcg ctccccggga ggtgacttca aggggaccgc
     8641 aggaccacct cgggggtggg gggagggctg cacacgcgga ccccgctccc cctccccaac
     8701 aaagcactgt ggaatcaaaa aggggggagg ggggatggag gggcgcgtca cacccccgcc
     8761 ccacaccctc acctcgaggt gagccccacg ttctgcttca ctctccccat ctc
//
