LOCUS       sequence-391834-       11039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of the centrosomal protein Akna fused to mOrange2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11039)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 11039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pBactin-Akna-mOrange2
FEATURES             Location/Qualifiers
     source          1..11039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1179..1201
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      1254..1263
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    3543..3554
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             5529..6239
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    6435..6569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6472..6491)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6526..6545
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6583..6599)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6583..6599)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6596..6618)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6607..6623
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6631..6637,6638..6655,6656..6661))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6676..6697
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6814..6831)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6985..7573)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7065..7084)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7744..8535,8536..8604))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8367..8386
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8605..8709)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8777..8795
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8833..8855)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8955..8974
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9168..9190
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9182..9199
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9183..9199
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(11000..11039,1..236)
                     /label=chicken beta-actin promoter
ORIGIN
        1 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
       61 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgrg gggcggggcg
      121 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      181 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      241 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      301 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      361 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      421 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      481 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      541 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
      601 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
      661 gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
      721 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
      781 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
      841 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
      901 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
      961 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1021 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1081 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgtccgca
     1141 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1201 cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagcta gcagccacca
     1261 tggccagctc aggggcaaag gcacaatggg ttgggccaag cctggggcaa gggccccgaa
     1321 ggcggcgctg ggcttgggct gaggaacaag atacagatgg aagaagagac cagggctggg
     1381 gaaacagcca gtctcttccc gaagccccca gtcctgagct cctagaggac ttccgtcggg
     1441 cacaggagca cctgccaccc ctggagtggg acccagacat gcaggactcc gaagagtctt
     1501 ctggagaaga gaccgaagct gacgatgcaa gcagcccaga gggttccacc gtgcctctgc
     1561 cctggctctc caggagcaac caacagctgg acatgtctga agaggagctg gatgaggcct
     1621 ctgggaggcc tgaggtagac ttagctggag agagttgtac ggagcttgag tgtgaagacc
     1681 agggagattc cagccctcca cctccaggac aggggccagc caaaggctgg gtcaccttta
     1741 tcaaacaagg cagtaactac agaccttcag agcatctcga ggcccagcca tctgttgaac
     1801 atagccgcac aaagtcttgg agcagtggaa ctgtgagcct ccgtcaacct agtgacagcc
     1861 ttggttctac ctgggaagga gataccgaag tccctcagcc ttccatcttg cccaaagccc
     1921 tgccacagag tccgtgccac aactttccac acccaggtga cagaaatgga ggtgacgttg
     1981 ctccggcaac acccacagaa ttccgggact ccttagcagc gccagcccag aatgccgagt
     2041 gctctgcagg cacgtggggg gaagaaacca ccagcctacc cagctctcgg cctgaggacc
     2101 aaacctggaa gaggactaag acatcaccta agccactgcc ctcccggttt actggttctg
     2161 tcagccccct gagtactcgg cttggggcaa taaagaaagt tgtgccccag cacaagcagg
     2221 gagccactct ggctggtcac tcttcttcac aagctcccaa gtatggcagg gggcgccgcc
     2281 tgaactaccc actccccgac ttctccaagg tggggccccg ggtgaggttt cccaaagatg
     2341 agaactaccg acccccgaag tccagaggcc acaacaggca acaaggctct accagacccc
     2401 tcatcttcaa gtcgccagct gagattgttc gggatgtact gttgagtagt ggagaggcgt
     2461 ccctggccaa ggaatcttct cttgctcaca ccatcaccag ggtgccccag gagttccaga
     2521 cacccgaaca agccactgag ctagttcatc agctccagga ggactatcac aagctcctca
     2581 ccaagtatgc ggaggctgag aacactatcg accaacttcg ccttggggcc aaggtgcacc
     2641 tgtattcaga cccaccacag cccagtcaga gcttctgctc ggggtcaatg ccacaaggaa
     2701 gcaaggtctt atccttctct atcccgcaac ccagagtagc agagtggtgg cctgaccctg
     2761 cccaggatcc acaggcctct gaggccacag ggtggccgtt cccacgaaca gacctgagtc
     2821 cctcctcgtc ccccggcgtg gccactccag ggcggcttcc tcagagccag ggcattgcca
     2881 cagaccagcc ctccacagga cagacccagg cactgacctc tcaggccagc cggctcctgg
     2941 ccaaggtgca gtccttcgag gaactggtac tggctggaca cctcccacct caggaccaaa
     3001 tcaagagctt ggagcaactg agggcagccc acatggccct agaggcagag tacctgcagg
     3061 cctgcagaga agagctcctg gaccctcagc ttgatgcctc tcaggggagt cccaggaccc
     3121 tcaacctctg cagggagcta gaagcagaga tataccacct gggacagcgc ctggaagagc
     3181 tacaggacca tatggaccag acccaaagag agacagagcc atgtaggcca gacctgcaag
     3241 acagcacccc aaccatgtcc tttctgcccc agtcagctca tctgtccatg ccttcaggac
     3301 ctgtctcctt gccagatggc caaacctacc aggagcctgc caccaccacc accacctccc
     3361 ctggaagctc ttgcacgctc cccatcaaca agaagttaag cctgagcatc aaaactgagg
     3421 aaagcccaag aggtctcccg gtcccactca gggacaggac gcttcaggtg gagcaggact
     3481 tccatggcct tctggagaga tacctcagcg ttaaatccct ccctgaagcc ttgagggatg
     3541 aagatgagga tgacctggag gaggaggaag aggagcagga ccatcaaggc cccttggaag
     3601 ttgatagtcc agccacagct ccagggaaaa cagaagccgt gagggtacca ccaggagagc
     3661 gcccaacaca ggctgaggaa agccataggg atgctaccca ggaggatgag gaacagatgg
     3721 gacctatgaa gtcaccagac ttccggccat ccatggccag agatacgtac acaccagtcc
     3781 tggatacagc tgaagtggct caacggggca ccaaagccat ggtgtcacat cagagcagtc
     3841 tgaccagcct ggaagagagc aggccctcag agctccttcc tcgaaaagcc ctgcttcggg
     3901 ctggtgggcc ccacactgag gagccctgga tggtgtcacc agagacagac agtggcttcg
     3961 tgggctcaga aaccagtata gtgtcaccct tcacccagac cccagagcac cggctctccc
     4021 atgtcagcac ttcaggacca tcagctcaac acctcactgc ctctgtgcct ggtgatagaa
     4081 cctcccatcc caaggccagg ggtctcatgg ttcccagaag agccactgag actggcatac
     4141 ccagaagccg aacccagcag catttctcca gcctgagctc tcccgggaga ggggcacaga
     4201 gctgccacct agaggagaca tcagtggcta aaatagcggt tcccaggtct gagttcaaga
     4261 gacagaagca gatttccaaa cagctccttc ccagtggaag aaccagcccc gactctgccc
     4321 cagctcccac agctgcctcc acgccccatg gatcagcaga gagcactgcc aacctcctcc
     4381 tcaacaggac agagcgcgac caggccatca aggatctgca ggcggaggtg tcacggctcc
     4441 gccttcaact ggaggacagc ctgcaccggc cacacccaga cggcccagcc tgtgtagctt
     4501 ctgctttcaa ccactccacc cagacccagg aaaagctggg ttcctcaccc tcctggggcc
     4561 cccactatgg cagtaaatcc acagagaggc tgtctaggga gcctaatggt gtagagccag
     4621 ctgagccaat gggaagacgt cgagccaggt cctcttcggt gcctcgggat gtacccagac
     4681 tgtacctaag ttctgaatcg gagtcgcctg cccctcggct ctcatctgag aagagcagga
     4741 cctttgagga gcaccccgag gctgcacagt gggggacaag accacagagc agctcgaagc
     4801 ggagggaaag agtttccttc cggggtcaat acacaggcca ggagtaccac attctatccc
     4861 caaaggccat cctgaaggac agtggcacac cgtcctgtcc ccactgccat cccattagga
     4921 cccaggacac aggcagtgct gtctctagag acaccacacg gggctcatct gccgctgaca
     4981 ccctccgctg tgccctgtgt ggtgaagtca agtcctctgc agaagcagat ggctccagct
     5041 caggtccctc tgagaagaac acccccaaaa agccttccac ccccatcctc aaacggaaga
     5101 acaggcagac gggctcgcca gtacgaatgg cccctggact ctggtacctg gctgctgcgc
     5161 ccccagcccc agccccaccg gctttggcct acatctcctc agctcccatt atgccttatc
     5221 taccacccac tgtgtactat gcagccccag cacctacctc agcccagaca gcttccccgc
     5281 agcctgctcg gggacctcgg agaacccggc actcagtcca actgggtctg aatgacctgg
     5341 aggagctgca ggccgcactc agagaggctg cacaggccgc cgagaacgtt cgctccacca
     5401 cccgccagct gagccgctcc ctgtctgcgg acctgcgcca tgcccgtagc ctgcgaggct
     5461 cctgcctttt cgtcgacggc agcaagcttg gtggaggcag cgcatcaggc ggaagcggaa
     5521 gcagatctat ggtgagcaag ggcgaggaga ataacatggc catcatcaag gagttcatgc
     5581 gcttcaaggt gcgcatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     5641 gcgagggccg cccctacgag ggctttcaga ccgctaagct gaaggtgacc aagggtggcc
     5701 ccctgccctt cgcctgggac atcctgtccc ctcatttcac ctacggctcc aaggcctacg
     5761 tgaagcaccc cgccgacatc cccgactact tcaagctgtc cttccccgag ggcttcaagt
     5821 gggagcgcgt gatgaactac gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     5881 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     5941 gccccgtgat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     6001 aggacggtgc cctgaagggc aagatcaaga tgaggctgaa gctgaaggac ggcggccact
     6061 acacctccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     6121 acatcgtcga catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     6181 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag
     6241 cggccgccat atgcatccta ggcctattaa tattccggag tatacgtagc cggctaacgt
     6301 taacaaccgg tacctctaga taagtaatga tcataatcag ccatatcaca tctgtagagg
     6361 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     6421 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6481 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6541 tcatcaatgt atcttatcat gtctggatca attcgtaatc atgtcatagc tgtttcctgt
     6601 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6661 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6721 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6781 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6841 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6901 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6961 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7021 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7081 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7141 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7201 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7261 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7321 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7381 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7441 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7501 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7561 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7621 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7681 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7741 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7801 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7861 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7921 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7981 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8041 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8101 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8161 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8221 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8281 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8341 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8401 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8461 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8521 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8581 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8641 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8701 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     8761 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     8821 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     8881 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     8941 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     9001 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
     9061 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     9121 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     9181 ttgtaaaacg acggccagtg ccaagttggg atctttgcat tggcccacgg ctctcaggat
     9241 ggggatgctc cccttcagca cccggttccc cttggaaact gatggtcctg gctctgtggc
     9301 atggcagtgg cactgtgagg agccccctac cagcagcaca cagtggggtt ggcactgcca
     9361 cgctccggat gccgcgctct gatccaaccc cataatcaag ggaacccgaa ttgccccatc
     9421 attgccccca ccacccccat cctgccgggc cctcacaccc cacgctgcct tctggtgaca
     9481 ttccccagcc caaacccacg gcttcatggc taccgcgggg catttcccat tgccgcccca
     9541 ttatcagctc tgcacacctc ccgctgtacc catgcctcgt ggctgccctt ctttgacgta
     9601 taatcttcta attaataccc ggccttgtca aagtggagca caaacgttaa ttaattcccc
     9661 agcaggcagg taattaacag tgtgactccc tttttgctgc gagtggggct gatacagaga
     9721 gatgtggcac tatggagccc acggggtcct ggcactgggt gcccacggag gtccccatgt
     9781 gctgcagtgt caccgcctcc gaggagacag tattgtccct gcggtgtccc tgcagctcag
     9841 ctctgtccac agggccacct ccagtttgga ggggacacaa tgcagccccg atgcaaccca
     9901 tcctcgcagc atcccaggga caaagacccc actgcaagac cgcacacagg gctgggtccc
     9961 gctcccctaa tatctacagt gcttcttgca tggcccctta atcaatgcag ttaatcagca
    10021 tgcgctcatg caccgctctg gagctgcaaa gcccctcgca gcgctgctca ccaacaccgc
    10081 gcaccgcccc ggcccagcct gcagcacgcg ctgcaaacag gaaagaaaca aaatattgcc
    10141 caaatgtagg caaaggcatt cggctgcctt gacctccgcc gggccgggcc ctgcctgact
    10201 cagctcctta ctcagcgctc gcttcctccc tccggctgcc accgccgcag cgcacaccct
    10261 gacaaagagt ggcccttaac gggctctgag gtgcacccag cagtgcactc agcagtccaa
    10321 gggccggcct ggaggtttgc accgctacgt gctgacatta gcattgaact tggccctggg
    10381 tagtgctgca ggccgggcgg ggtgggtgta gagagtgcag cgcgcgttgc acccggtgcc
    10441 ccttcccctc ccttgcatcc cagcaggctg caccccagca ccaggcccgt gcatgcatgc
    10501 tcctggtgtt attgcagcct ggtgcatgca tgcgtcttga tggtgcagcg ctgtgcatgc
    10561 atcctccttg gtgtgtagca gtctagtgca tgcatacccc tcggtgttat tgctgctctg
    10621 tgcacgcacg ctcattgtat cacttcatcc cagtgcatgc actcacactg gagcgattgc
    10681 tgctcggtgc acgcacactc attgtatcac tgcagctcag tggctgcacg cacaccggtg
    10741 ttattgctgc tcggtgcgtg catgcacatc agtgtcgctg cagctcagtg catgcacgct
    10801 cattgcccat cgctatccct gcctctcctg ctggcgctcc ccgggaggtg acttcaaggg
    10861 gaccgcagga ccacctcggg ggtgggggga gggctgcaca cgcggacccc gctccccctc
    10921 cccaacaaag cactgtggaa tcaaaaaggg gggagggggg atggaggggc gcgtcacacc
    10981 cccgccccac accctcacct cgaggtgagc cccacgttct gcttcactct ccccatctc
//
