LOCUS       sequence-391837-       15067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a 
            template for Akap9 containing constructs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15067)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 15067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pCR-XL-rAkap9
FEATURES             Location/Qualifiers
     source          1..15067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     297..319
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     311..328
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     312..328
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     335..354
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        335..353
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12088..12104)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12088..12104)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12101..12123)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12112..12128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12136..12142,12143..12160,12161..12166))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12181..12202
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12319..12336)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12490..13078)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12570..12589)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13216..13590)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      13587..13596
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(13796..14590)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(13796..14590)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13907..13926)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     14517..14536
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(join(14939..15067,1..174))
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(15041..15060)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg
       61 tgcactggcc agggggatca ccatccgtcg ccccggcgtg tcaataatat cactctgtac
      121 atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa actgccgtac
      181 gtataggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
      241 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
      301 gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt
      361 gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc agaattcgcc
      421 ctttctttcc tctccactgc gtcccagcct accctgcaga cgccatggag gacgaggaga
      481 ggcaaaggaa gctggcagct ggcaaggcga agcttgctcg gtttcgacaa agaaaagccc
      541 agtgcgatgg ggacattcca aagaagcaga agaagaaaag aactccgagt tctaaacaag
      601 attcaagctt acataccgat cagcagagcg ctgagatctg ctcggaaagc tctcagaggg
      661 ccgactcagc tgggagtcct gattcctcca gtgcagaaaa agcaaagcac ggccaggtct
      721 tcgcagctga tcctgaaagt gaaatttcta ccactgccga tgagtgtagc tctgagatta
      781 atggctgcaa ttctgtgacg aaaccaggaa atcctacaga taccttaagg gaagaagatt
      841 tttccattga cgattcttat tctgaacaag gagctcagtc cagtcagacc tgtcttcaga
      901 tgggggaaaa cgagttggct gagaagcagc atgaggtcga agagttgact caggagttgg
      961 aagaaatgag ggccagcttt gggacggaag ggctgaagca gttacaagaa ttcgaagccg
     1021 ccattaaaca aagagatggc atcatcactc agctcactgc taacttgcag caagccagaa
     1081 aagaaaagga tgacacaacg atagaatttt tagagttgac agaacagagt caaaagctgc
     1141 agattcagtt ccagcactta caggctaatg aaaccctgca aaacagcact cttagtcgaa
     1201 ctgcaacaga tttactgcag gctaaacgac agatctttac tctgcagcaa cagctgcaag
     1261 attatcagaa aaaggaggag gacgtccaag cacaaatttc ttttttacaa gagaaaatta
     1321 gaatgttgga aatggaaaaa gacagaaaag tggaaagctt aaatacaaaa gaaatagagg
     1381 aaaagcaggc aatcattgag gagttgaata caagagtaat agaagaagag agaaaaactg
     1441 ctgagctaga gaataaagtg acaactgctg atgaattact aggacagctc caaggacagg
     1501 ttacagaaaa ggatcaggag atcaaaagcc tgaaactaga actggtcaac tctcagcaga
     1561 acgagcggaa gtgttcagag gaaatcaaag agctaatgag gacagttgaa gaactccaga
     1621 agagaaatct caaggacagc cagctgggga ccggcaccgt gcgaagggtg gaacaagaaa
     1681 cacaacggaa actaagcctt ctccaggcag agctggatga gatgtatggg aagcagatag
     1741 tgcaaatgaa acaagaatta atgaaacagc acatgtctca gatagaggag ctcaaatccc
     1801 agcacaagag ggacttggag aacaccttga agtcagacgc aagcactaca gtcagtaaag
     1861 agcaggtaaa cttgatgagt gcggccataa gcgaactcaa tgtaagactg caggatactc
     1921 atgctcaaaa ggaaaaactc aagggagagc tcggggcagc tctgggagaa aaatcagctc
     1981 tacagagtca gtttgaagac ctgttagaag aggtcaggtt tttacgagag caagtgcaga
     2041 aggctagaca gacaatagca gaacaggaaa acagactgag cgatgcgcgc aagtccctga
     2101 gcacagtgga agacttaaaa gctgagatcg ttgctgcctc cgagtccaga aaggagctcg
     2161 aggtcaagca cgaggctgag attacaaatt acaaaataaa actcgaaatg ctagaaaaag
     2221 aaaaaaatgc cgtgctagac agaatggcgg aatcccaaga agcagagttg gagaggctga
     2281 ggacacagct cctcttcagc cacgaggaag aactgtccaa actgaaagaa gacttagagg
     2341 tcgaacatcg aataaacgtt gagaagctta aagataattt gggcatccac tacaagcagc
     2401 agatagacgg cttgcaaaat gaaatgaata ggaagatgga gactatgcag tgcgaaacag
     2461 acaacctgat aactcggcag aatcagctaa ttctggaaaa ctcaaagcta agagatttac
     2521 aggaacacct cgttaactca aaatcagaag agatgaatct tcagatcaac gagctgcaaa
     2581 aagagattga aatcctcaga cgagaagaga aggaaaaagg tagccttgaa caggaagtcc
     2641 aggggctaca gcttaaaact gaacaactgg agagacaact gaaggagaag ggagatgacc
     2701 ttcaagaaaa atgtgcacag ctggacgcag agaacagcgt tcttaaagat gagaagaaag
     2761 cccttgaaga catgttgaaa atgtacactc ctctggacca agaggatccc agcacacgca
     2821 agtcggaaga cccgaggtgg caaaaggaag tagggatgct gaggaaggag aacgaggacc
     2881 ttcagcagca atgccttcat ctaagcgaag agatcgagaa gcagaagaat acctttgcgt
     2941 ttgctgagaa gaactttgaa gttaactatc aggagttaca gagggagtat acttgtcttc
     3001 tcaagataag agacgactta gaagacacac tagctaagca ggccttgcag tatgaaagta
     3061 agctgagcgc gctggaggaa gagctccatt cgcaaagagg aagtccgacc acgccaccga
     3121 agggggagaa ttctgggttt gctgccaaca gaactttccc attggagccg ctggaagtcg
     3181 gcgaagttgt agaaaaagat acaacagagc ttatggagaa gctggaagtg accaggcgag
     3241 agaagttgga gctctcagag aaagtctctg gtctttctga acaactaaag cagacacatg
     3301 gtacgattga ttctctaagt gaagaggtca gagccttaaa gcaggagaag gaacagcttt
     3361 tattgagatg tggagagcta gaactcctca ctaatgctaa tgggacagaa aatgcatccg
     3421 tctgccctgt gcagaggagt ccttaccaag ctgggcctgt gatggggacg gtcagggatt
     3481 ctcagggacc tatttctaaa gtcagcgaag atttggctga agaatcaaaa ccaatgacag
     3541 aagataaaat tccctttaaa gaaagtggga gagaaccgtt gcttttgcca acaagaacaa
     3601 aaaaaccatc tcgagtgaca gttgaaccat gtgaaagtga aaaactccag caagagcttt
     3661 atgccctcaa agccgagcag gacgatctga ggctgcagat ggaagcccag cgcctgtgcc
     3721 tttttgtggt ttattcaact catgcggatc aggttcgtgc atatatggag aaggagagag
     3781 aggaagctct ttgcagcctt aaagatgagc tcgtttccgc tcaacagaaa aagatcgacg
     3841 agcttcataa gatacaccag tgccagctcc agcatgttaa agtacaggag acaggtaacg
     3901 agcctttaca ggtgctcatt gaacggcttc aaaaggcagt gtctgagaaa tgctttcaca
     3961 tctcaaagac cctaaacaat gttttcgatg aatgttatac tcctttaaaa tgtgaaatga
     4021 atatagaaga gaaagagaat tctggtgttt acacttctca aaatcaaagc ccagaattac
     4081 gagagtatag atacgaagtc caagactttc aagaaaacat gcaaatcctt ctcggtaaag
     4141 taacggaaga gtgcagcaag ctcttggtac tgcagacacg tttaaacaag attaatgaac
     4201 aacaagttga cggtgtgaca ttcgagtttg ctgaagaaaa tgtagcagaa gtggaagcag
     4261 gcctcctgtc aggccgttct cagagtggct tacagggtgc agatgtcagt catgagagca
     4321 aggtgtctga gtatgagagg caagtccagg agctacagag cctcgtggcc gccggacagc
     4381 tgcagctgaa ggaaaccgaa gcaaactaca gagcagaaat ccagtgtttg caggaaagac
     4441 ttcaggctgt cagtgaggcc acagttcagc cgagcttatc catggactca gtggtgttta
     4501 aaggatctgg tgcccagaaa ccagtgtact gtgggagctg tctaagagag caggtggacg
     4561 gcacagcaga gttttctgat ggctttgaag tgaggcaaga aacaaatatg gttaaattga
     4621 tggaaaagca gtatcaagag cgcttggaag aagaaatagc taaggtcatc gtatcaatga
     4681 gtatagcatt tgctcaacaa actgaactct ctagattatc tgaggagaaa gaaaatgtga
     4741 tacaatccga acaagcacat gctctgtgtt ctcaaaatga acaccattta aatgacatca
     4801 catcacaatg tcaagttggt cttcagactg ttgaggcaac tggcaagaat ttaaaagaag
     4861 aatttaaacc acttagcaaa gagttaggag aatatagaaa agcagttcct ttgtctaatc
     4921 atgatgatct tgatgatgta ctaaagtcag aagaacatgg actgactatt tcagaagaaa
     4981 tattttccaa agatgaaaca tttttagtga gaaattctag tcctgatgag gtgctggtgt
     5041 caaatatgga tacttctaga cagctcatcc taaatgaaca gctggaagat atgcggcaag
     5101 agcttgtacg acagtatgaa gaacatcagc aggcaacaga actgttgcgg caggcccaca
     5161 tgcaacaaat ggagagacaa cgagaagacc aggagcagct acaagaggag atcaagaggc
     5221 tcaaccagca gttaacccag agatcctcca tagatactga gcacgtggtc tcagagagag
     5281 aaagggttct gttagaggag ctggaagcac taaagcggct gtctctagct ggaagaaagg
     5341 agctgtgttg tgagctgcag cacagcagta cgcaaacaca ggatggaaat gacaatcaag
     5401 aagttgagga gcagacatta aaagacaaga cactggagag aaaacctgaa gacatacctc
     5461 tcgatataaa tctgtccaat gaaaggtacg ctctcaaaaa agctaataat agacttctga
     5521 agatcctgtt agatgttgtc aagacaacat cagctgctga agaaacaatt ggccgtcatg
     5581 ttcttggaat tctggaccga tctagtaaaa gtcagccagc taccagcctg ctttggagat
     5641 cagaaacaga ggcatccgcc acagtttgtg tacaagagga gtgtgcaaga gctatggatg
     5701 agtccgtacc ctcttaccct ggaagtgcca tagcaacaca tgacagcgtt tggtcaaaag
     5761 tcaccgagga aggggcagag ctgtcacagc ggctcgtgag gagtggcttt gctgggcctg
     5821 taatagatcc tgaaaatgaa gaacttatgc tgaacatcag ctctcgacta caagcagctg
     5881 tcgaaaaact cctagaagcc ataagtgaga ccagcagtca gcttgaacat gcgaaagtta
     5941 cacagacaga gttgatgcgt gagtcattta gacaaaaaca agaggcaaca gaatcgcttc
     6001 attgcctgga agaactgcgg gagcgccttc aggaggagtc cagggccaga gagcagctgg
     6061 ctgtcgagct aaataaagct gagagtgtca ttgatgggta ctcggatgaa aaaactctct
     6121 ttgaaaggca aattcaagaa aaaaccgata taatagagca tcttgaacag gagttattat
     6181 gtacaaacaa cagactccaa gagctggagt cagaccagcg ccggatggaa gaggaacgag
     6241 agctgctgtc caggcagagg gaggcgatga gggcagatgc tggcccggtg gagcagcaat
     6301 ttctacagga gactgaaaaa ctaatgaagg aaaagctgga agtgcagtgt caagctgaga
     6361 aagtccgcgg cgacctacag aagcaagtga aagctctaga aatagatgtt gaagaacagg
     6421 tcagcaggtt tatagaactg gagcaagaga aaaacgccga gctcatagat ctaaggcagc
     6481 aaagccaagc actggaaaag caactagaaa aaatgaggaa attcttagac gagcaggcaa
     6541 tcgacagaga gcatgagaga gatgtttttc agcaggaaat acagaagcta gagcatcaac
     6601 ttaaggctgt acctagactc ctgcctgtga gtgagcacca aaccagagag gttgagcagc
     6661 taactaatca cctgaaagag aaaacagaca aatgcagtga gctgctgctg tctaaggagc
     6721 aactgcagcg agacgtgcag gagaggaatg aagagattga gaagctggag tgccgagtgc
     6781 gagagctgga gcaagccctg ctggctactg cagacccctt cccaaaggta gaggaacaga
     6841 aaaggcctgg agctgtagaa gtggacccag aattatcgct agaagttcaa ctgcaggctg
     6901 agagggatgc tacagataga aaacagaaag agatcaccaa cttagaagaa caattagagc
     6961 agtttcggga agaactggaa aataagaatg aagaggttca ggagcttctc atgcagctag
     7021 aaattcagaa aaaggagtcc actacccgcc tgcaagagct ccagcaagaa aacagattat
     7081 ttaaggagga aatagaaaaa ctcgggttca ccatgaagga atctgattct ctgtcgactc
     7141 aggatcagcc catgctgttt ggcaaatttg cgcagctaat acaagaaaag gagattgaaa
     7201 ttgaccgatt aaatgagcag tttataaaac tccagcagca gcttaaactc acaacagata
     7261 acaaggtcat tgaagaacaa aaggaccaga tacgagattt agaaacccag attgaatgtt
     7321 tgatgagtga acaagaactt atgaagaaac agagagcaga ggaagcagag caactcaatg
     7381 gcgtgattga gaaattgcag caggagttag tcagcactga gcagaagaga gagggtgcca
     7441 gggccctccc agaagacgag gagagcttta aacaccagtt ggacagggtg actgttgaaa
     7501 agctgggttt agaaaagcaa gtagagacca ccaaccagga actgacagac ttgaaaaatg
     7561 tacttaaaga aatcaacttc aaaatggatc acatgacaca agcactgtgc aacttgaata
     7621 aagaacttcc caatgtccct aaggaaagtg tccacgtgac tgtgcatgag cttggctgtg
     7681 acaaactgca gccagaaggt gcctctgccc aggatgcttc ccagccccta gaaagtcaga
     7741 cctcccttat gtgtcttcaa gaaagcacta aagcttctca aatactagaa ataaagtcac
     7801 taccactgca ggggtctggt agcaccaagg acttcgagct tgcccagtgt cataaacaaa
     7861 tagacaccat acaagagcaa gaccagtctg aaataggaat gcttcagaag aagatcacaa
     7921 acctgcagaa gatcctggag aagttctcgg ctgcattggt cagttgggtc caaatggagg
     7981 cagctcagga ccgtgtgcag ctctaccaag aaaagcaaac gcagacggtc tcgtcagcac
     8041 ctgaaaggac agatacacag aatgtaaatt gtctggcaga aaacaacttg gagtcacatg
     8101 tccccacact ggctgtgaga ccagcagagt tggagagccg tgtagctgaa gtgcttagtg
     8161 atataatgtc agaaaaacac atggaaactg tggggaagaa tgcctcagag acagaaaagg
     8221 aagttgtggc gctacagaag ctattagaag aggccaagaa aaggctgaaa gaagaaagag
     8281 aacaaagccc tagggataga gagagaactg ctcagctaac tcatgctggt ggaggcagtg
     8341 gattttcagg tggccttgaa acttttgaag ctgagtcagc cacttcagaa ctcgccagtt
     8401 acaaagaaag ggcagaaaaa cttgaagaag atcttttggt aaaagagagc tacataacat
     8461 cacttcagaa agacctaggc caagttaaag atcaactcac agaggccaag gagaaattat
     8521 cccactactt agaaaaagag aatgggactg gggaacagga aagcagaagg gtccccatac
     8581 cagagtcacc ttctgttgag ggaggtggcg gctctgggac catggagagg acgggcaagg
     8641 tcagcagcag caatcagacc ccgcagatcc ttgttagaca tgctggggtt cagatcgact
     8701 tacagagcga gcgctcccaa gaagaactca gagacaccat caaccagttt actgaaaaga
     8761 tggagcgcat acaagagctc catgctgctg agattttgga tatggaatcc agacacatct
     8821 tagaaaccga gtctttgaag aaagaacatt ctgttgcgat ccagttactg actaaggagt
     8881 gtgagaccct gaaggatatg acccagtacc tgagatgcaa aaagggatcc tccatttctg
     8941 atttggcaga ttctgttgct tatcaaagta gagaagtata ctccagtgat tcagaatctg
     9001 actggggtca aagccaggga tttgatacag caacagaagg gcgagaagaa ggcgaaactt
     9061 cagatttatt tccaaagaaa ataaaggggt tagtgaaagc tgtccacagt gagggcatgc
     9121 aggtgctttc tctctcgaat cccctctgtg atgatggaga ggaccgttcc gttcagcagc
     9181 tttcagaatc ttggctgaaa gagagacaga cttacctcaa taccatctcg tcccttaaag
     9241 atttaatttc taaaatgcaa gtgcaaagag aaactgaggt ctatgacagc tgtcagtctc
     9301 atgacagctt ctcagactgg cgaggtgaac ttctgcttgc ctgtcagcgc gttttcataa
     9361 aggaacgtag tgttttacta gctacattcc aaactgagct gacgtctctc agcactagag
     9421 atgcagacgg agtattaaac tctctagaac aaagaataca agagcagggc attgaatatc
     9481 aaacagcaat ggactgtctt caaaaagcag ataggaggag cttactgact gaaatagaag
     9541 atctgcgtgc tcaaattaat ggcaggaaaa tgactctgga aagagaacaa gagattgaga
     9601 aacccagcca agagctcctg gactgtaatc tgcagcagaa gcagtcccac gtgctggaaa
     9661 tgcaattgga actcggcagc ctgagagaca gagcagccga gctgcaggaa cagctgagtt
     9721 cggaaaagat ggtggttgcg gaactaaaaa gcgaacttgc acaggcaaag ctggaattag
     9781 gaacaacact caaggcccag cataagcgct taaaggagtt ggaagcattc aggtcagaaa
     9841 ttaaggagaa gaccgatgaa attcatttcc ttagtgatac attagcaagg gaacagaaga
     9901 agtcactaga gctgcagtgg gctttggaaa aagaaaaggc caagtcagga caccacgagg
     9961 aatgggaaaa agaagaactt gaggatctga aattttcact cgaagatcaa aaacggaaaa
    10021 atacacagtt aaacctcctt ttggaacaac agaagcagct actaaatgaa tctcaacaga
    10081 aaatggagtc acagaaaatg ttgcacgatg cccagttatc agaagagcaa ggccgcaact
    10141 tagggcttca ggctctactg gagtcagagc aagtcagggt ccaggagatg aagagcaccc
    10201 tggacaagga gcgggagctg tatgcccagc tgcagagccg tgatgatggc gggcagcccc
    10261 tgccagtcct gccttctgag gacctactta aagagctaca gaaacaactg gaggaaaaac
    10321 acacccgcat agtagaactc ttaagtgaaa cagaaaaata caaactagac tccttgcaaa
    10381 caaggcaaca gatggaaaag gacagagagg ttcaccagaa gacactgcag acagaacagg
    10441 aggcaaacac ccagggccaa aagaaaatgc aggagctgca gtccaaagtg gaggagctgc
    10501 agcgccagct gcaggagaaa aggcagcagg tatataagct agacctagag gggaagcgac
    10561 tgcaggggat catgcaggaa ttccaaaagc aggagctgga acccgaggaa aaacggggaa
    10621 gcagaggact tgtgtatcag aatcttaatg agcccgccag ctggaccttc actgacgacc
    10681 ggactagaaa ttgggttctt caacagaaga tgggagagac caaggacacg aacttcacaa
    10741 agttgattga aataaatgga gaacttgacc ataatcatga cttggaagtg atcagacaga
    10801 cacttcagca tgtggcttca aagctgcagc atgtggcaca gaaagcctgc aacaggctac
    10861 agtttgaaac agcaagtgat gatgcattca tctggattca ggagaatatc gatggaatta
    10921 ttttgcaact acagaaactg actggccagc caggtgacga gcacagctta gcacccccca
    10981 gttcttcttg cggctcctta acggaaagcc taatgagaca aaatactgag ctaacaagac
    11041 ttatcaacca actgacggaa gaaaagaaca ccttaaggag catagtggtg aagctggagg
    11101 agctcaacag gtgtcactgg cacacgggag ccggcagaga ctgttgttct aggttttcat
    11161 ttattgatcc cgctgacatc gaagccataa tcgcttcaga aaaggaagtc tggaacagag
    11221 aaaagctatc tcttcagaaa gcgttgaaaa gggcagaggc gaaagtgtat aaactgaaag
    11281 ctgaactgcg caatgatgct ttgctgcgga gtctgagccc tgactctgag catgccgccc
    11341 tgcagaaaat ttataacaaa tacctgagag cttcaagttt ccgcaaggct ctcatatatc
    11401 agaaaaaata cctcctgctg ctcctggggg gcttccagga gtgtgaggac gccaccctgg
    11461 gggttctggc ccgaatgggg ggacacccag ccttgaaaga tcccaagaca atcaccagcc
    11521 acccgaaggg gttcacccgg ttccgctctg ccgtcagagt gtctattgcc atctcaagaa
    11581 tgaaattttt ggtgcggcgg tggcagcaag tgacaagtac tagctccatc aatattaaca
    11641 gggatggctt tggcctgagt ccaggcattg aaaagactga cccattctat cattcgtctg
    11701 gtgggctgga gctgtatgga gaaccaagac acacaacata ccgctcaaga tttgatctgg
    11761 actaccctag gtctcccttg ccattgcaga ataggtaccc gggtgctcca gacttaaatt
    11821 ctacttcaat ggcttcttct caacttcacc agtacaatcc tgataaatct ctgacagact
    11881 atgtcactcg gctagaggca ctgaaaaggc gacttggagc tatacagtca gggtcgacta
    11941 ctcaatttca ttttggcatg aggagataat ccaagggcga attccagcac actggcggcc
    12001 gttactagtg gatccgagct cggtaccaag cttgatgcat agcttgagta ttctaacgcg
    12061 tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    12121 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    12181 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    12241 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    12301 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    12361 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    12421 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    12481 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    12541 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    12601 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    12661 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    12721 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    12781 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    12841 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    12901 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    12961 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    13021 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    13081 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    13141 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    13201 aagttttagc acgtgtcagt cctgctcctc ggccacgaag tgcacgcagt tgccggccgg
    13261 gtcgcgcagg gcgaactccc gcccccacgg ctgctcgccg atctcggtca tggccggccc
    13321 ggaggcgtcc cggaagttcg tggacacgac ctccgaccac tcggcgtaca gctcgtccag
    13381 gccgcgcacc cacacccagg ccagggtgtt gtccggcacc acctggtcct ggaccgcgct
    13441 gatgaacagg gtcacgtcgt cccggaccac accggcgaag tcgtcctcca cgaagtcccg
    13501 ggagaacccg agccggtcgg tccagaactc gaccgctccg gcgacgtcgc gcgcggtgag
    13561 caccggaacg gcactggtca acttggccat ggtggccctc ctcacgtgct attattgaag
    13621 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    13681 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgatgcgg tgtgaaatac
    13741 cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta ataattcaga
    13801 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
    13861 aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag
    13921 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
    13981 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga
    14041 gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc
    14101 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
    14161 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
    14221 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
    14281 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
    14341 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
    14401 cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
    14461 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
    14521 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
    14581 gttcaatcat gcgaaacgat cctcatcctg tctcttgatc agagcttgat cccctgcgcc
    14641 atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac
    14701 cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta
    14761 gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc
    14821 ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg
    14881 ctttctacgt gaaaaggatc taggtgaaga tcctttttga taatctcatg cctgacattt
    14941 atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat
    15001 cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc
    15061 gccagct
//
