LOCUS       sequence-391839-        8161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of the gamma-tubulin fused to mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8161)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 8161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pBactin-Tubg1-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..8161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1179..1201
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             2651..3361
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      2906..2915
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3557..3691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3594..3613)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3648..3667
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3705..3721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3705..3721)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3718..3740)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3729..3745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3753..3759,3760..3777,3778..3783))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3798..3819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3936..3953)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4107..4695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4187..4206)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4866..5657,5658..5726))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5489..5508
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5727..5831)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5899..5917
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5955..5977)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6077..6096
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6290..6312
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6304..6321
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6305..6321
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(8122..8161,1..236)
                     /label=chicken beta-actin promoter
ORIGIN
        1 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
       61 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgrg gggcggggcg
      121 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      181 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      241 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      301 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      361 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      421 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      481 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      541 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
      601 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
      661 gkgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
      721 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
      781 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
      841 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
      901 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
      961 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1021 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1081 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgtccgca
     1141 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1201 cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagcta gcctcgagaa
     1261 ttctgcctga ggagcgatgc cgagagaaat catcacccta cagttgggcc agtgcggcaa
     1321 tcagattggg ttcgagttct ggaaacagct atgtgccgag catggcatca gtcccgaggg
     1381 catcgtagaa gagttcgcca cggagggcac tgaccgaaag gacgtctttt tctaccaggc
     1441 agatgatgag cactacatcc cccgggctgt gctgctggac ctggagcccc gggtgatcca
     1501 ttccatccta aactcctcct atgctaagct ctacaaccca gagaacatct acctgtcgga
     1561 gcatggcgga ggagctggca acaactgggc cagcggattc tcccagggag aaaaaatcca
     1621 cgaggacatt tttgacatca tagatcggga ggcagatggc agtgacagtc tagagggatt
     1681 tgtactgtgt cattccattg ctggggggac aggctctggc ttgggctcct acctcctgga
     1741 acggctaaat gacaggtacc ccaaaaaact agtgcagaca tactctgtgt tccccaacca
     1801 ggatgagatg agtgacgtgg tggtccaacc ctacaactcc ctcctcacac taaagaggct
     1861 gacccagaac gcggactgtg tggtggtgct ggacaacacg gccctgaacc tgatagccac
     1921 agaccgccta cacatccaga acccatcctt ctcccagatc aaccagctgg tgtccaccat
     1981 catgtcagcc agcaccacca ccctgcgcta ccctggatac atgaacaatg acctcatcgg
     2041 cctcatcgcc tcgctcattc ccacccctcg gctccacttt ctcatgactg gctatacccc
     2101 cctcaccacg gaccagtcag tggccagtgt gaggaagaca acagtcctgg atgtcatgag
     2161 gcgcctgcta cagcccaaga atgtgatggt gtccacaggc cgggatcgtc agaccaacca
     2221 ctgctacatc gccatcctca acatcatcca gggagaggtg gaccccaccc aggtccacaa
     2281 gagcctgcag aggatccggg aaaggaaact ggccaacttc atcccctggg gcccagccag
     2341 catccaggtg gccctgtcaa ggaagtctcc ctacctaccc tcagcccacc gggtcagcgg
     2401 gctcatgatg gccaaccaca ccagtatctc ctcgctcttc gaacggacct gtcgccagtt
     2461 tgacaagctg cggaaacggg aggccttcat ggagcagttc cgcaaggagg acatcttcaa
     2521 ggacaatttt gacgagatgg acacctccag ggagattgtg cagcagctga ttgacgagta
     2581 ccacgcggcc acacggccag actatatctc ctggggcacc caggagcagg tcgacggaag
     2641 tggtggatcc atggtgagca agggcgagga ggataacatg gcctctctcc cagcgacaca
     2701 tgagttacac atctttggct ccatcaacgg tgtggacttt gacatggtgg gtcagggcac
     2761 cggcaatcca aatgatggtt atgaggagtt aaacctgaag tccaccaagg gtgacctcca
     2821 gttctccccc tggattctgg tccctcatat cgggtatggc ttccatcagt acctgcccta
     2881 ccctgacggg atgtcgcctt tccaggccgc catggtagat ggctccggct accaagtcca
     2941 tcgcacaatg cagtttgaag atggtgcctc ccttactgtt aactaccgct acacctacga
     3001 gggaagccac atcaaaggag aggcccaggt gaaggggact ggtttccctg ctgacggtcc
     3061 tgtgatgacc aactcgctga ccgctgcgga ctggtgcagg tcgaagaaga cttaccccaa
     3121 cgacaaaacc atcatcagta cctttaagtg gagttacacc actggaaatg gcaagcgcta
     3181 ccggagcact gcgcggacca cctacacctt tgccaagcca atggcggcta actatctgaa
     3241 gaaccagccg atgtacgtgt tccgtaagac ggagctcaag cactccaaga ccgagctcaa
     3301 cttcaaggag tggcaaaagg cctttaccga tgtgatgggc atggacgagc tgtacaagta
     3361 agcggccgcc atatgcatcc taggcctatt aatattccgg agtatacgta gccggctaac
     3421 gttaacaacc ggtacctcta gataagtaat gatcataatc agccatatca catctgtaga
     3481 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     3541 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     3601 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     3661 actcatcaat gtatcttatc atgtctggat caattcgtaa tcatgtcata gctgtttcct
     3721 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3781 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     3841 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3901 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3961 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4021 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4081 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4141 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4201 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4261 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4321 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4381 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4441 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4501 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4561 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4621 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4681 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4741 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4801 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4861 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4921 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4981 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5041 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5101 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5161 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5221 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5281 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5341 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5401 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5461 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5521 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5581 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5641 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5701 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5761 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5821 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     5881 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     5941 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     6001 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     6061 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     6121 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     6181 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     6241 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     6301 cgttgtaaaa cgacggccag tgccaagttg ggatctttgc attggcccac ggctctcagg
     6361 atggggatgc tccccttcag cacccggttc cccttggaaa ctgatggtcc tggctctgtg
     6421 gcatggcagt ggcactgtga ggagccccct accagcagca cacagtgggg ttggcactgc
     6481 cacgctccgg atgccgcgct ctgatccaac cccataatca agggaacccg aattgcccca
     6541 tcattgcccc caccaccccc atcctgccgg gccctcacac cccacgctgc cttctggtga
     6601 cattccccag cccaaaccca cggcttcatg gctaccgcgg ggcatttccc attgccgccc
     6661 cattatcagc tctgcacacc tcccgctgta cccatgcctc gtggctgccc ttctttgacg
     6721 tataatcttc taattaatac ccggccttgt caaagtggag cacaaacgtt aattaattcc
     6781 ccagcaggca ggtaattaac agtgtgactc cctttttgct gcgagtgggg ctgatacaga
     6841 gagatgtggc actatggagc ccacggggtc ctggcactgg gtgcccacgg aggtccccat
     6901 gtgctgcagt gtcaccgcct ccgaggagac agtattgtcc ctgcggtgtc cctgcagctc
     6961 agctctgtcc acagggccac ctccagtttg gaggggacac aatgcagccc cgatgcaacc
     7021 catcctcgca gcatcccagg gacaaagacc ccactgcaag accgcacaca gggctgggtc
     7081 ccgctcccct aatatctaca gtgcttcttg catggcccct taatcaatgc agttaatcag
     7141 catgcgctca tgcaccgctc tggagctgca aagcccctcg cagcgctgct caccaacacc
     7201 gcgcaccgcc ccggcccagc ctgcagcacg cgctgcaaac aggaaagaaa caaaatattg
     7261 cccaaatgta ggcaaaggca ttcggctgcc ttgacctccg ccgggccggg ccctgcctga
     7321 ctcagctcct tactcagcgc tcgcttcctc cctccggctg ccaccgccgc agcgcacacc
     7381 ctgacaaaga gtggccctta acgggctctg aggtgcaccc agcagtgcac tcagcagtcc
     7441 aagggccggc ctggaggttt gcaccgctac gtgctgacat tagcattgaa cttggccctg
     7501 ggtagtgctg caggccgggc ggggtgggtg tagagagtgc agcgcgcgtt gcacccggtg
     7561 ccccttcccc tcccttgcat cccagcaggc tgcaccccag caccaggccc gtgcatgcat
     7621 gctcctggtg ttattgcagc ctggtgcatg catgcgtctt gatggtgcag cgctgtgcat
     7681 gcatcctcct tggtgtgtag cagtctagtg catgcatacc cctcggtgtt attgctgctc
     7741 tgtgcacgca cgctcattgt atcacttcat cccagtgcat gcactcacac tggagcgatt
     7801 gctgctcggt gcacgcacac tcattgtatc actgcagctc agtggctgca cgcacaccgg
     7861 tgttattgct gctcggtgcg tgcatgcaca tcagtgtcgc tgcagctcag tgcatgcacg
     7921 ctcattgccc atcgctatcc ctgcctctcc tgctggcgct ccccgggagg tgacttcaag
     7981 gggaccgcag gaccacctcg ggggtggggg gagggctgca cacgcggacc ccgctccccc
     8041 tccccaacaa agcactgtgg aatcaaaaag gggggagggg ggatggaggg gcgcgtcaca
     8101 cccccgcccc acaccctcac ctcgaggtga gccccacgtt ctgcttcact ctccccatct
     8161 c
//
