LOCUS       sequence-391840-        8206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of the gamma-tubulin fused to enhanced (E)-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8206)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 8206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pBactin-Tubg1-mEGFP
FEATURES             Location/Qualifiers
     source          1..8206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1179..1201
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(2687..2689,2690..2692,2693..3406)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2732..2753)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2993..3012)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3340..3361
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3602..3736
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3639..3658)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3693..3712
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3750..3766)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3750..3766)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3763..3785)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3774..3790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3798..3804,3805..3822,3823..3828))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3843..3864
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3981..3998)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4152..4740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4232..4251)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4911..5702,5703..5771))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5534..5553
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5772..5876)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5944..5962
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6000..6022)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6122..6141
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6335..6357
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6349..6366
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6350..6366
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(8167..8206,1..236)
                     /label=chicken beta-actin promoter
ORIGIN
        1 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
       61 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg
      121 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      181 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      241 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      301 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      361 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      421 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      481 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      541 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
      601 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
      661 gkgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
      721 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
      781 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
      841 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
      901 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
      961 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1021 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1081 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgtccgca
     1141 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1201 cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagcta gcctcgagaa
     1261 ttctgcctga ggagcgatgc cgagagaaat catcacccta cagttgggcc agtgcggcaa
     1321 tcagattggg ttcgagttct ggaaacagct atgtgccgag catggcatca gtcccgaggg
     1381 catcgtagaa gagttcgcca cggagggcac tgaccgaaag gacgtctttt tctaccaggc
     1441 agatgatgag cactacatcc cccgggctgt gctgctggac ctggagcccc gggtgatcca
     1501 ttccatccta aactcctcct atgctaagct ctacaaccca gagaacatct acctgtcgga
     1561 gcatggcgga ggagctggca acaactgggc cagcggattc tcccagggag aaaaaatcca
     1621 cgaggacatt tttgacatca tagatcggga ggcagatggc agtgacagtc tagagggatt
     1681 tgtactgtgt cattccattg ctggggggac aggctctggc ttgggctcct acctcctgga
     1741 acggctaaat gacaggtacc ccaaaaaact agtgcagaca tactctgtgt tccccaacca
     1801 ggatgagatg agtgacgtgg tggtccaacc ctacaactcc ctcctcacac taaagaggct
     1861 gacccagaac gcggactgtg tggtggtgct ggacaacacg gccctgaacc tgatagccac
     1921 agaccgccta cacatccaga acccatcctt ctcccagatc aaccagctgg tgtccaccat
     1981 catgtcagcc agcaccacca ccctgcgcta ccctggatac atgaacaatg acctcatcgg
     2041 cctcatcgcc tcgctcattc ccacccctcg gctccacttt ctcatgactg gctatacccc
     2101 cctcaccacg gaccagtcag tggccagtgt gaggaagaca acagtcctgg atgtcatgag
     2161 gcgcctgcta cagcccaaga atgtgatggt gtccacaggc cgggatcgtc agaccaacca
     2221 ctgctacatc gccatcctca acatcatcca gggagaggtg gaccccaccc aggtccacaa
     2281 gagcctgcag aggatccggg aaaggaaact ggccaacttc atcccctggg gcccagccag
     2341 catccaggtg gccctgtcaa ggaagtctcc ctacctaccc tcagcccacc gggtcagcgg
     2401 gctcatgatg gccaaccaca ccagtatctc ctcgctcttc gaacggacct gtcgccagtt
     2461 tgacaagctg cggaaacggg aggccttcat ggagcagttc cgcaaggagg acatcttcaa
     2521 ggacaatttt gacgagatgg acacctccag ggagattgtg cagcagctga ttgacgagta
     2581 ccacgcggcc acacggccag actatatctc ctggggcacc caggagcagg tcgacggcag
     2641 caagcttggt ggaggcagcg catcaggcgg aagcggaagc agatctatgg tgagcaaggg
     2701 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     2761 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2821 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     2881 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     2941 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     3001 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     3061 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     3121 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     3181 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     3241 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     3301 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3361 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagcgg ccgccatatg
     3421 catcctaggc ctattaatat tccggagtat acgtagccgg ctaacgttaa caaccggtac
     3481 ctctagataa gtaatgatca taatcagcca tatcacatct gtagaggttt tacttgcttt
     3541 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     3601 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3661 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3721 ttatcatgtc tggatcaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat
     3781 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     3841 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     3901 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     3961 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     4021 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     4081 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4141 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4201 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4261 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4321 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4381 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4441 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4501 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4561 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     4621 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4681 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4741 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4801 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4861 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4921 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4981 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     5041 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     5101 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     5161 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5221 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     5281 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     5341 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     5401 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5461 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5521 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5581 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     5641 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5701 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5761 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5821 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5881 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     5941 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     6001 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     6061 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     6121 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     6181 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     6241 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     6301 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     6361 gccagtgcca agttgggatc tttgcattgg cccacggctc tcaggatggg gatgctcccc
     6421 ttcagcaccc ggttcccctt ggaaactgat ggtcctggct ctgtggcatg gcagtggcac
     6481 tgtgaggagc cccctaccag cagcacacag tggggttggc actgccacgc tccggatgcc
     6541 gcgctctgat ccaaccccat aatcaaggga acccgaattg ccccatcatt gcccccacca
     6601 cccccatcct gccgggccct cacaccccac gctgccttct ggtgacattc cccagcccaa
     6661 acccacggct tcatggctac cgcggggcat ttcccattgc cgccccatta tcagctctgc
     6721 acacctcccg ctgtacccat gcctcgtggc tgcccttctt tgacgtataa tcttctaatt
     6781 aatacccggc cttgtcaaag tggagcacaa acgttaatta attccccagc aggcaggtaa
     6841 ttaacagtgt gactcccttt ttgctgcgag tggggctgat acagagagat gtggcactat
     6901 ggagcccacg gggtcctggc actgggtgcc cacggaggtc cccatgtgct gcagtgtcac
     6961 cgcctccgag gagacagtat tgtccctgcg gtgtccctgc agctcagctc tgtccacagg
     7021 gccacctcca gtttggaggg gacacaatgc agccccgatg caacccatcc tcgcagcatc
     7081 ccagggacaa agaccccact gcaagaccgc acacagggct gggtcccgct cccctaatat
     7141 ctacagtgct tcttgcatgg ccccttaatc aatgcagtta atcagcatgc gctcatgcac
     7201 cgctctggag ctgcaaagcc cctcgcagcg ctgctcacca acaccgcgca ccgccccggc
     7261 ccagcctgca gcacgcgctg caaacaggaa agaaacaaaa tattgcccaa atgtaggcaa
     7321 aggcattcgg ctgccttgac ctccgccggg ccgggccctg cctgactcag ctccttactc
     7381 agcgctcgct tcctccctcc ggctgccacc gccgcagcgc acaccctgac aaagagtggc
     7441 ccttaacggg ctctgaggtg cacccagcag tgcactcagc agtccaaggg ccggcctgga
     7501 ggtttgcacc gctacgtgct gacattagca ttgaacttgg ccctgggtag tgctgcaggc
     7561 cgggcggggt gggtgtagag agtgcagcgc gcgttgcacc cggtgcccct tcccctccct
     7621 tgcatcccag caggctgcac cccagcacca ggcccgtgca tgcatgctcc tggtgttatt
     7681 gcagcctggt gcatgcatgc gtcttgatgg tgcagcgctg tgcatgcatc ctccttggtg
     7741 tgtagcagtc tagtgcatgc atacccctcg gtgttattgc tgctctgtgc acgcacgctc
     7801 attgtatcac ttcatcccag tgcatgcact cacactggag cgattgctgc tcggtgcacg
     7861 cacactcatt gtatcactgc agctcagtgg ctgcacgcac accggtgtta ttgctgctcg
     7921 gtgcgtgcat gcacatcagt gtcgctgcag ctcagtgcat gcacgctcat tgcccatcgc
     7981 tatccctgcc tctcctgctg gcgctccccg ggaggtgact tcaaggggac cgcaggacca
     8041 cctcgggggt ggggggaggg ctgcacacgc ggaccccgct ccccctcccc aacaaagcac
     8101 tgtggaatca aaaagggggg aggggggatg gaggggcgcg tcacaccccc gccccacacc
     8161 ctcacctcga ggtgagcccc acgttctgct tcactctccc catctc
//
