LOCUS       sequence-391844-       15322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 
            (Akap9) fused to AcGFP. Used to displace endogenous Akap9 from 
            Golgi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15322)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 15322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pBactin-AcGFP-128-425-Akap9
FEATURES             Location/Qualifiers
     source          1..15322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1263..1272
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1269..1985
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    3057..3191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3205..3221)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3229..3245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3253..3259,3260..3277,3278..3283))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3298..3319
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3607..4195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(4366..5157,5158..5226))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5227..5331)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5805..5821
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7622..7897
                     /label=chicken beta-actin promoter
     regulatory      8924..8933
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8930..9646
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    10718..10852
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10866..10882)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10890..10906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10914..10920,10921..10938,10939..10944))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10959..10980
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(11268..11856)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(12027..12818,12819..12887))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12888..12992)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13466..13482
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(15283..15322,1..236)
                     /label=chicken beta-actin promoter
ORIGIN
        1 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
       61 gggggcgggg gggggggggg cgcgcgccag gcggggcggg gcggggcgrg gggcggggcg
      121 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
      181 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      241 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      301 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      361 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      421 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      481 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      541 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
      601 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
      661 gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
      721 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
      781 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
      841 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
      901 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
      961 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1021 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1081 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgtccgca
     1141 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1201 cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagcta gcgctaccgg
     1261 tcgccaccat ggtgagcaag ggcgccgagc tgttcaccgg catcgtgccc atcctgatcg
     1321 agctgaatgg cgatgtgaat ggccacaagt tcagcgtgag cggcgagggc gagggcgatg
     1381 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cctgtgccct
     1441 ggcccaccct ggtgaccacc ctgagctacg gcgtgcagtg cttctcacgc taccccgatc
     1501 acatgaagca gcacgacttc ttcaagagcg ccatgcctga gggctacatc caggagcgca
     1561 ccatcttctt cgaggatgac ggcaactaca agtcgcgcgc cgaggtgaag ttcgagggcg
     1621 ataccctggt gaatcgcatc gagctgaccg gcaccgattt caaggaggat ggcaacatcc
     1681 tgggcaataa gatggagtac aactacaacg cccacaatgt gtacatcatg accgacaagg
     1741 ccaagaatgg catcaaggtg aacttcaaga tccgccacaa catcgaggat ggcagcgtgc
     1801 agctggccga ccactaccag cagaataccc ccatcggcga tggccctgtg ctgctgcccg
     1861 ataaccacta cctgtccacc cagagcgccc tgtccaagga ccccaacgag aagcgcgatc
     1921 acatgatcta cttcggcttc gtgaccgccg ccgccatcac ccacggcatg gatgagctgt
     1981 acaagtccgg aggtggtgga ggtatggggg aaaacgagtt ggctgagaag cagcatgagg
     2041 tcgaagagtt gactcaggag ttggaagaaa tgagggccag ctttgggacg gaagggctga
     2101 agcagttaca agaattcgaa gccgccatta aacaaagaga tggcatcatc actcagctca
     2161 ctgctaactt gcagcaagcc agaaaagaaa aggatgacac aacgatagaa tttttagagt
     2221 tgacagaaca gagtcaaaag ctgcagattc agttccagca cttacaggct aatgaaaccc
     2281 tgcaaaacag cactcttagt cgaactgcaa cagatttact gcaggctaaa cgacagatct
     2341 ttactctgca gcaacagctg caagattatc agaaaaagga ggaggacgtc caagcacaaa
     2401 tttctttttt acaagagaaa attagaatgt tggaaatgga aaaagacaga aaagtggaaa
     2461 gcttaaatac aaaagaaata gaggaaaagc aggcaatcat tgaggagttg aatacaagag
     2521 taatagaaga agagagaaaa actgctgagc tagagaataa agtgacaact gctgatgaat
     2581 tactaggaca gctccaagga caggttacag aaaaggatca ggagatcaaa agcctgaaac
     2641 tagaactggt caactctcag cagaacgagc ggaagtgttc agaggaaatc aaagagctaa
     2701 tgaggacagt tgaagaactc cagaagagaa atctcaagga cagccagctg gggaccggca
     2761 ccgtgcgaag ggtggaacaa gaaacacaac ggaaactaag ccttctccag gcagagctgg
     2821 atgagatgta tgggaagcag atagtgcaaa tgaaacaaga attaatgaaa cagcacatgt
     2881 ctcagataga ggagctttga cgagtcgacg gtaccgcggg cccgggatcc accggatcta
     2941 gataagtaat gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa
     3001 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     3061 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3121 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     3181 atgtctggat caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     3241 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3301 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3361 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3421 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3481 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3541 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3601 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3661 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3721 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3781 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3841 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3901 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3961 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4021 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4081 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4141 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4201 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4261 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4321 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4381 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4441 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4501 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4561 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4621 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4681 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4741 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4801 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     4861 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     4921 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     4981 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5041 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5101 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5161 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5221 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5281 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5341 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     5401 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     5461 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     5521 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     5581 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     5641 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     5701 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     5761 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     5821 tgccaagttg ggatctttgc attggcccac ggctctcagg atggggatgc tccccttcag
     5881 cacccggttc cccttggaaa ctgatggtcc tggctctgtg gcatggcagt ggcactgtga
     5941 ggagccccct accagcagca cacagtgggg ttggcactgc cacgctccgg atgccgcgct
     6001 ctgatccaac cccataatca agggaacccg aattgcccca tcattgcccc caccaccccc
     6061 atcctgccgg gccctcacac cccacgctgc cttctggtga cattccccag cccaaaccca
     6121 cggcttcatg gctaccgcgg ggcatttccc attgccgccc cattatcagc tctgcacacc
     6181 tcccgctgta cccatgcctc gtggctgccc ttctttgacg tataatcttc taattaatac
     6241 ccggccttgt caaagtggag cacaaacgtt aattaattcc ccagcaggca ggtaattaac
     6301 agtgtgactc cctttttgct gcgagtgggg ctgatacaga gagatgtggc actatggagc
     6361 ccacggggtc ctggcactgg gtgcccacgg aggtccccat gtgctgcagt gtcaccgcct
     6421 ccgaggagac agtattgtcc ctgcggtgtc cctgcagctc agctctgtcc acagggccac
     6481 ctccagtttg gaggggacac aatgcagccc cgatgcaacc catcctcgca gcatcccagg
     6541 gacaaagacc ccactgcaag accgcacaca gggctgggtc ccgctcccct aatatctaca
     6601 gtgcttcttg catggcccct taatcaatgc agttaatcag catgcgctca tgcaccgctc
     6661 tggagctgca aagcccctcg cagcgctgct caccaacacc gcgcaccgcc ccggcccagc
     6721 ctgcagcacg cgctgcaaac aggaaagaaa caaaatattg cccaaatgta ggcaaaggca
     6781 ttcggctgcc ttgacctccg ccgggccggg ccctgcctga ctcagctcct tactcagcgc
     6841 tcgcttcctc cctccggctg ccaccgccgc agcgcacacc ctgacaaaga gtggccctta
     6901 acgggctctg aggtgcaccc agcagtgcac tcagcagtcc aagggccggc ctggaggttt
     6961 gcaccgctac gtgctgacat tagcattgaa cttggccctg ggtagtgctg caggccgggc
     7021 ggggtgggtg tagagagtgc agcgcgcgtt gcacccggtg ccccttcccc tcccttgcat
     7081 cccagcaggc tgcaccccag caccaggccc gtgcatgcat gctcctggtg ttattgcagc
     7141 ctggtgcatg catgcgtctt gatggtgcag cgctgtgcat gcatcctcct tggtgtgtag
     7201 cagtctagtg catgcatacc cctcggtgtt attgctgctc tgtgcacgca cgctcattgt
     7261 atcacttcat cccagtgcat gcactcacac tggagcgatt gctgctcggt gcacgcacac
     7321 tcattgtatc actgcagctc agtggctgca cgcacaccgg tgttattgct gctcggtgcg
     7381 tgcatgcaca tcagtgtcgc tgcagctcag tgcatgcacg ctcattgccc atcgctatcc
     7441 ctgcctctcc tgctggcgct ccccgggagg tgacttcaag gggaccgcag gaccacctcg
     7501 ggggtggggg gagggctgca cacgcggacc ccgctccccc tccccaacaa agcactgtgg
     7561 aatcaaaaag gggggagggg ggatggaggg gcgcgtcaca cccccgcccc acaccctcac
     7621 ctcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
     7681 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
     7741 gcgcgcgcca ggcggggcgg ggcggggcgr ggggcggggc ggggcgaggc ggagaggtgc
     7801 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
     7861 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg
     7921 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
     7981 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
     8041 ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc cttaaagggc tccgggaggg
     8101 ccctttgtgc gggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg
     8161 ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     8221 gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg
     8281 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
     8341 gtgtgggcgc ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag
     8401 cacggcccgg cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg
     8461 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     8521 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     8581 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     8641 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     8701 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     8761 cgccgtcccc ttctccatct ccagcctcgg ggctgtccgc agggggacgg ctgccttcgg
     8821 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggggt ttatatcttc
     8881 ccttctctgt tcctccgcag cccccaagct agcgctaccg gtcgccacca tggtgagcaa
     8941 gggcgccgag ctgttcaccg gcatcgtgcc catcctgatc gagctgaatg gcgatgtgaa
     9001 tggccacaag ttcagcgtga gcggcgaggg cgagggcgat gccacctacg gcaagctgac
     9061 cctgaagttc atctgcacca ccggcaagct gcctgtgccc tggcccaccc tggtgaccac
     9121 cctgagctac ggcgtgcagt gcttctcacg ctaccccgat cacatgaagc agcacgactt
     9181 cttcaagagc gccatgcctg agggctacat ccaggagcgc accatcttct tcgaggatga
     9241 cggcaactac aagtcgcgcg ccgaggtgaa gttcgagggc gataccctgg tgaatcgcat
     9301 cgagctgacc ggcaccgatt tcaaggagga tggcaacatc ctgggcaata agatggagta
     9361 caactacaac gcccacaatg tgtacatcat gaccgacaag gccaagaatg gcatcaaggt
     9421 gaacttcaag atccgccaca acatcgagga tggcagcgtg cagctggccg accactacca
     9481 gcagaatacc cccatcggcg atggccctgt gctgctgccc gataaccact acctgtccac
     9541 ccagagcgcc ctgtccaagg accccaacga gaagcgcgat cacatgatct acttcggctt
     9601 cgtgaccgcc gccgccatca cccacggcat ggatgagctg tacaagtccg gaggtggtgg
     9661 aggtatgggg gaaaacgagt tggctgagaa gcagcatgag gtcgaagagt tgactcagga
     9721 gttggaagaa atgagggcca gctttgggac ggaagggctg aagcagttac aagaattcga
     9781 agccgccatt aaacaaagag atggcatcat cactcagctc actgctaact tgcagcaagc
     9841 cagaaaagaa aaggatgaca caacgataga atttttagag ttgacagaac agagtcaaaa
     9901 gctgcagatt cagttccagc acttacaggc taatgaaacc ctgcaaaaca gcactcttag
     9961 tcgaactgca acagatttac tgcaggctaa acgacagatc tttactctgc agcaacagct
    10021 gcaagattat cagaaaaagg aggaggacgt ccaagcacaa atttcttttt tacaagagaa
    10081 aattagaatg ttggaaatgg aaaaagacag aaaagtggaa agcttaaata caaaagaaat
    10141 agaggaaaag caggcaatca ttgaggagtt gaatacaaga gtaatagaag aagagagaaa
    10201 aactgctgag ctagagaata aagtgacaac tgctgatgaa ttactaggac agctccaagg
    10261 acaggttaca gaaaaggatc aggagatcaa aagcctgaaa ctagaactgg tcaactctca
    10321 gcagaacgag cggaagtgtt cagaggaaat caaagagcta atgaggacag ttgaagaact
    10381 ccagaagaga aatctcaagg acagccagct ggggaccggc accgtgcgaa gggtggaaca
    10441 agaaacacaa cggaaactaa gccttctcca ggcagagctg gatgagatgt atgggaagca
    10501 gatagtgcaa atgaaacaag aattaatgaa acagcacatg tctcagatag aggagctttg
    10561 acgagtcgac ggtaccgcgg gcccgggatc caccggatct agataagtaa tgatcataat
    10621 cagccatatc acatctgtag aggttttact tgctttaaaa aacctcccac acctccccct
    10681 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
    10741 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10801 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaattcgta
    10861 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    10921 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    10981 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    11041 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    11101 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    11161 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    11221 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    11281 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    11341 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    11401 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    11461 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    11521 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    11581 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    11641 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    11701 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    11761 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    11821 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    11881 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    11941 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    12001 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    12061 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    12121 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    12181 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    12241 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    12301 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    12361 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    12421 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    12481 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    12541 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    12601 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    12661 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    12721 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    12781 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    12841 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    12901 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    12961 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    13021 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    13081 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
    13141 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
    13201 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
    13261 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
    13321 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
    13381 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
    13441 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagtt gggatctttg
    13501 cattggccca cggctctcag gatggggatg ctccccttca gcacccggtt ccccttggaa
    13561 actgatggtc ctggctctgt ggcatggcag tggcactgtg aggagccccc taccagcagc
    13621 acacagtggg gttggcactg ccacgctccg gatgccgcgc tctgatccaa ccccataatc
    13681 aagggaaccc gaattgcccc atcattgccc ccaccacccc catcctgccg ggccctcaca
    13741 ccccacgctg ccttctggtg acattcccca gcccaaaccc acggcttcat ggctaccgcg
    13801 gggcatttcc cattgccgcc ccattatcag ctctgcacac ctcccgctgt acccatgcct
    13861 cgtggctgcc cttctttgac gtataatctt ctaattaata cccggccttg tcaaagtgga
    13921 gcacaaacgt taattaattc cccagcaggc aggtaattaa cagtgtgact ccctttttgc
    13981 tgcgagtggg gctgatacag agagatgtgg cactatggag cccacggggt cctggcactg
    14041 ggtgcccacg gaggtcccca tgtgctgcag tgtcaccgcc tccgaggaga cagtattgtc
    14101 cctgcggtgt ccctgcagct cagctctgtc cacagggcca cctccagttt ggaggggaca
    14161 caatgcagcc ccgatgcaac ccatcctcgc agcatcccag ggacaaagac cccactgcaa
    14221 gaccgcacac agggctgggt cccgctcccc taatatctac agtgcttctt gcatggcccc
    14281 ttaatcaatg cagttaatca gcatgcgctc atgcaccgct ctggagctgc aaagcccctc
    14341 gcagcgctgc tcaccaacac cgcgcaccgc cccggcccag cctgcagcac gcgctgcaaa
    14401 caggaaagaa acaaaatatt gcccaaatgt aggcaaaggc attcggctgc cttgacctcc
    14461 gccgggccgg gccctgcctg actcagctcc ttactcagcg ctcgcttcct ccctccggct
    14521 gccaccgccg cagcgcacac cctgacaaag agtggccctt aacgggctct gaggtgcacc
    14581 cagcagtgca ctcagcagtc caagggccgg cctggaggtt tgcaccgcta cgtgctgaca
    14641 ttagcattga acttggccct gggtagtgct gcaggccggg cggggtgggt gtagagagtg
    14701 cagcgcgcgt tgcacccggt gccccttccc ctcccttgca tcccagcagg ctgcacccca
    14761 gcaccaggcc cgtgcatgca tgctcctggt gttattgcag cctggtgcat gcatgcgtct
    14821 tgatggtgca gcgctgtgca tgcatcctcc ttggtgtgta gcagtctagt gcatgcatac
    14881 ccctcggtgt tattgctgct ctgtgcacgc acgctcattg tatcacttca tcccagtgca
    14941 tgcactcaca ctggagcgat tgctgctcgg tgcacgcaca ctcattgtat cactgcagct
    15001 cagtggctgc acgcacaccg gtgttattgc tgctcggtgc gtgcatgcac atcagtgtcg
    15061 ctgcagctca gtgcatgcac gctcattgcc catcgctatc cctgcctctc ctgctggcgc
    15121 tccccgggag gtgacttcaa ggggaccgca ggaccacctc gggggtgggg ggagggctgc
    15181 acacgcggac cccgctcccc ctccccaaca aagcactgtg gaatcaaaaa ggggggaggg
    15241 gggatggagg ggcgcgtcac acccccgccc cacaccctca cctcgaggtg agccccacgt
    15301 tctgcttcac tctccccatc tc
//
