LOCUS       sequence-391859-        5619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-EBNA3A
FEATURES             Location/Qualifiers
     source          1..5619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3498..3597)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3614..3633)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3615..3633)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3638..3654)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3638..3654)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4085..4876
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4969..5557
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5458..5477
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gacaaggaca ggccgggtcc cccggccctg gatgacaaca tggaagaaga
      721 agtcccatct acctcggttg tgcaggaaca ggtatcggcg ggagattggg aaaatgtcct
      781 catagagtta tcagatagca gctcagaaaa ggaagcagaa gatgcccacc tggagccggc
      841 ccagaagggt acgaagagaa agcgggtcga tcatgatgcc ggtgggtcag ctccagcacg
      901 acccatgctc ccaccccagc cggatctccc tgggcgagaa gccattctcc gcaggtttcc
      961 actagatcta agaacacttc ttcaagcgat tggagccgcg gctacgcgca tcgacacacg
     1021 agccatagac cagtttttcg gatcccagat ttcaaatacc gagatgtaca taatgtatgc
     1081 catggccatt cgacaggcca ttagagatcg tcggagaaat ccagcttctc gtagagatca
     1141 ggccaaatgg agactgcaaa ccctggccgc cggatggcct atgggttacc aggcatacag
     1201 cagctggatg tacagctaca ccgatcacca gacgactccc acattcgtac atctccaggc
     1261 gacacttggg tgcacaggtg gccgtaggtg tcacgtgacc ttttccgccg gcacctttaa
     1321 gctgccgcga tgtacccccg gggatcgcca gtggttgtat gttcagagct ccgtgggtaa
     1381 cattgtacag agctgtaatc cccgctacag tattttcttt gactatatgg ctatacacag
     1441 gagcctcacg aaaatctggg aggaagtttt aacacctgac cagcgtgttt catttatgga
     1501 attcctagga tttttgcaga gaacggattt gtcctatatc aagagctttg tcagcgatgc
     1561 cctgggcacc actagtatcc aaacaccgtg gatcgatgac aatcctagca cggagacggc
     1621 acaggcttgg aatgccggct ttctccgggg tcgtgcgtat gggatagact tgcttagaac
     1681 tgaaggggaa catgtcgaag gtgctaccgg tgaaacgcga gaagaaagtg aggacacgga
     1741 gagcgatgga gatgatgaag atcttccttg tatagtgtcc agaggtggac ctaaggtcaa
     1801 acgaccccct atatttataa gacgtctgca caggttgctg ctgatgagag cgggcaaacg
     1861 aacagaacag ggcaaggagg tactggaaaa ggcccgtggg agcacttatg gcacacctag
     1921 gccgcctgtt ccgaaaccaa gaccagaggt cccacaaagc gacgagacag ctaccagtca
     1981 cgggtcggcg caagtcccag aacccccaac cattcaccta gcagctcagg gaatggcata
     2041 cccattacat gaacaacacg gcatggcccc gtgtccggta gcacaggccc cacctacgcc
     2101 cttgccccct gtatctccag gggatcaact cccaggtgtt tttagcgacg ggcgagtggc
     2161 gtgtgcacca gtacccgccc cggctgggcc tattgtccgg ccctgggagc catccctgac
     2221 acaggctgcg gggcaggcct ttgcacccgt tagaccacaa cacatgccag tagaacccgt
     2281 ccctgtcccg acagtggcac ttgagcgacc agtttacccc aagccagttc gtccggcacc
     2341 tcctaagatt gctatgcagg gccccgggga aacttctggc attagacgcg cgcgggagcg
     2401 ttggaggccc gcaccttgga cgccaaatcc accccgttct cccagtcaga tgtccgtgcg
     2461 tgaccgtctg gctcgtttgc gtgctgaggc acaggtcaaa caggctagtg ttgaggtgca
     2521 gcccccccag ttgacccaag tatcccctca gcaaccaatg gaggggccgt tggtaccaga
     2581 gcagcagatg ttccctggtg ccccctttag ccaggttgct gatgtggtcc gggcacctgg
     2641 ggtaccggcg atgcagccac agtactttga cctcccctta attcaaccca ttagccaggg
     2701 ggcacccgtg gccccgttga gggctagtat gggcccggta cctccggtac cggcaacaca
     2761 gccacagtat tttgacatcc ccttaactga acccattaac cagggggcat ccgcggccca
     2821 ttttctccct cagcaaccga tggaggggcc gttggtacct gagcagtgga tgttcccagg
     2881 tgccgccctg agccagagtg ttaggccagg ggtagcgcag tcacaatatt ttgacctccc
     2941 cttaactcaa cccattaacc atggggcacc cgcagcccat ttcctccatc agccaccaat
     3001 ggaggggccg tgggtacccg agcagtggat gttccaaggt gcccccccta gccaaggcac
     3061 tgacgtggtc caacatcagc tggatgcttt ggggtataca ctccatggtc ttaaccatcc
     3121 cggggttccc gtgtctcctg ccgttaacca atatcatctc agccaggctg cctttgggtt
     3181 acctattgat gaggatgaga gtggcgaggg gtccgatacc tccgagccgt gtgaagctct
     3241 tgatttgtca atccatggca ggccctgccc tcaggccccc gagtggcctg ttcaagagga
     3301 gggtggccag gatgccaccg aggttcttga tttgtcaatc catggcaggc cccgccctcg
     3361 gacccccgag tggcctgttc aaggggaagg tggccaaaat gtcacaggcc ctgaaactag
     3421 aagggtggtg gtgtcagctg ttgttcacat gtgtcaggat gacgagtttc cggatctaca
     3481 agatcctcca gatgaggccc caactttctt gtacaaagtt ggcattataa gaaagcattg
     3541 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     3601 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     3661 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     3721 cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg
     3781 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     3841 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     3901 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     3961 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     4021 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     4081 cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     4141 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     4201 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     4261 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     4321 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     4381 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     4441 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     4501 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     4561 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     4621 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     4681 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     4741 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     4801 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     4861 ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc
     4921 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4981 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5041 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5101 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5161 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5221 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5281 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5341 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5401 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5461 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5521 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5581 acggttcctg gccttttgct ggccttttgc tcacatgtt
//
