LOCUS       sequence-391860-        5458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inactivation of G protein subunits through Pertussis toxin (PTX).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pJR019
FEATURES             Location/Qualifiers
     source          1..5458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    36..130
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    169..263
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    302..396
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     primer_bind     complement(1841..1869)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2617..2637
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        3203..3307
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3308..3376,3377..4168)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3526..3545)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4339..4927
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4828..4847
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5081..5098
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5215..5236
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5251..5256,5257..5274,5275..5281)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5289..5305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5294..5316
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5313..5329
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5313..5329
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcta gcccatgatt acgccaagct acgggcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagactcc ctagcccatg attacgccaa gctacgggcg gagtactgtc
      181 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagac tccctagccc atgattacgc caagctacgg
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactccctag gggcggccgc
      421 actgactggg ccggccaaaa atgcataagg ttttgctggc actgttcttt atctttctgg
      481 caccagcatc cgcactggca gtctccgaac cggcctgcag gtcgattttt tgcaaattac
      541 gagcgttgta gggggcggag cgataggtcc tataggtttt ggtatatcat cattcattca
      601 ttcattggta cattcattta cccaccttcc tctttctgag cttctctgga gttctgtgct
      661 tcctttttcc cttatcttta tactgtaatt tttaactttc aggcattgat tggcgcgcct
      721 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      781 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt aaaaatggac
      841 gatcctcccg ccaccgtata ccgctatgac tcccgcccgc cggaggacgt tttccagaac
      901 ggattcacgg cgtggggaaa caacgacaat gtgctcgacc atctgaccgg acgttcctgc
      961 caggtcggca gcagcaacag cgctttcgtc tccaccagca gcagccggcg ctataccgag
     1021 gtctatctcg aacatcgcat gcaggaagcg gtcgaggccg aacgcgccgg caggggcacc
     1081 ggccacttca tcggctacat ctacgaagtc cgcgccgaca acaatttcta cggcgccgcc
     1141 agctcgtact tcgaatacgt cgacacttat ggcgacaatg ccggccgtat cctcgccggc
     1201 gcgctggcca cctaccagag cgaatatctg gcacaccggc gcattccgcc cgaaaacatc
     1261 cgcagggtaa cgcgggtcta tcacaacggc atcaccggcg agaccacgac cacggagtat
     1321 tccaacgctc gctacgtcag ccagcagact cgcgccaatc ccaaccccta cacatcgcga
     1381 aggtccgtag cgtcgatcgt cggcacattg gtgcgcatgg cgccggtgat aggcgcttgc
     1441 atggcgcggc aggccgaaag ctccgaggcc atggcagcct ggtccgaacg cgccggcgag
     1501 gcgatggttc tcgtgtacta cgaaagcatc gcgtattcgt tctagggtac cgtagctgtc
     1561 tcatcctact ttcacctagt taactgcttg tcttaaaatc tatgcttctc tttagtatct
     1621 aaaattttcc tagaagctta caagtatata aatggtctct tctcaataaa ggttgtatat
     1681 ttattcatct tattgaatct gccatttcct cgtttttgcg agtttatata ccttccaatt
     1741 ttctttctat tgtattttca acttctaatt ttaattcagg gaaactgctg taccggtaga
     1801 aaaaatgctc gagagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
     1861 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     1921 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     1981 ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc agccaacact
     2041 tgtcactact ttctgttatg gtgttcaatg cttctcgaga tacccagatc atatgaaacg
     2101 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
     2161 caaagatgac gggaactaca agacacgtaa gtttaaacag ttcggtacta actaaccata
     2221 catatttaaa ttttcaggtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
     2281 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
     2341 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
     2401 tgtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc agaacttcaa
     2461 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     2521 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     2581 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     2641 tgctgggatt acacatggca tggatgaact atacaaatag gaattcgcta gccggccata
     2701 caagtaatcc ggatgatcga cgccaacgtc gttgaatttt caaattttaa atactgaata
     2761 tttgtttttt ttcctattat ttatttattc tctttgtgtt ttttttcttg ctttctaaaa
     2821 aattaattca atccaaatct aaacattttt ttttctcttt ccgtctccca attcgtattc
     2881 cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt catttcatta
     2941 ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga aatgaaataa
     3001 ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt aaaagaaaac
     3061 ataaaaatat atagaggagg aaggaaaact agtaaaaaat aagcaaagaa attaggcgaa
     3121 cgatgagaat tgtcctcgct tgggcccaaa aggccttttt tttttggcgc cgacgtcagg
     3181 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     3241 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     3301 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     3361 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     3421 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     3481 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     3541 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     3601 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     3661 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     3721 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     3781 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     3841 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     3901 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     3961 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     4021 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     4081 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     4141 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     4201 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4261 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4321 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4381 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4441 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     4501 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4561 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4621 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4681 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4741 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4801 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4861 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4921 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4981 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     5041 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     5101 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     5161 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     5221 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     5281 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     5341 caagctgtaa gtttaaacat gatcttacta actaactatt ctcatttaaa ttttcagagc
     5401 ttaaaaatgg ctgaaatcac tcacaacgat ggatacgcta acaacttgga aatgaaat
//
