LOCUS       sequence-391861-        5790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BPLF1_1501-2500
FEATURES             Location/Qualifiers
     source          1..5790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3669..3768)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3785..3804)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3786..3804)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3809..3825)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3809..3825)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4256..5047
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5140..5728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5629..5648
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg ttgggaataa cgagggtgag gagggagaga ctgcgatccc tgctcgtgga
      721 cgatggtggt gcggcggaga gaatggaggc cgcggaaccc ggctggttca cggatatcga
      781 gaccggccct ctggccagac tggacgcttg gcccgccacc cccgcggcta ccgccaaaga
      841 aggaggagga gggcgaggag cggaggaagc ggccggggcc ctctttcgcg ccaggacggc
      901 ggccgatgcc atccgttcgg ccctcgcgca gacgcgccag gccctgcagt ccccggacat
      961 gaaatcagcg gtggtcaaca cggatctgga ggccccctac gcggagtacg agcgggggct
     1021 ggccgggctt ctggaaaaaa gacgagcagc cgaggctgcc ctgacggcca tcgtgagcga
     1081 gtacgtggat cggacgctac ccgaagccac taatgaccca ggccaggcta acctgcctcc
     1141 tcctccaact atcccccagg caaccgcccc gcccaggctg gcctcggact cggcgctctg
     1201 gcctaagaag ccccagctgc tgacaaggcg agagcgggac gatctcctcc aggccacggg
     1261 ggacttcttc tcggagctgc tgaccgaggc cgaggcggcc gaggtccggg cgctggaaga
     1321 gcaggtccgg gagagccaga ccctgatggc gaaggcccac gagatggcgg caagcactcg
     1381 gcggggcttt cacacggctc tggaggccgt cctctccagg tcacgcgacg aagcccccga
     1441 cgatgaactc cggagcctgc ttccctcccc gcccaaagcc cctgtccagg ctcccctcga
     1501 ggccgccctg gcccgggcag cggccgggaa cggctcatgg ccctaccgga aatccctggc
     1561 agccgccaag tggatccggg gcatctgcga ggccgtgcgg ggtctttccg aaggagccct
     1621 ggccttagct gggggcgcgg gtgcctggct gaacctagcc gctgcggctg acggtgaaat
     1681 tcatgagcta acacggctcc tggaagtcga gggcatggcc cagaactcca tggatggcat
     1741 ggaggagctg aggttggctc tggccacgtt ggaccccaag cgggtcgcgg ggggcaagga
     1801 gaccgtcgca gactggaaaa ggcgcctctc ccgactggag gccatcatcc aggaggccca
     1861 ggaagagtcc cagctgcagg gaacgctgca ggatctggtc acccaggcca ggggccacac
     1921 cgacccgcgc cagctcaaga tcgtggtgga ggctgccagg ggcctggcgc ttggggcctc
     1981 ggctggctcc cagtatgccc tcctcaagga caagctgctg cgctatgcct cggccaagca
     2041 gagtttcctg gctttttacg agaccgccca gcctaccgtt ttcgttaagc atcccttgac
     2101 caacaacctg cccctcctca tcacgatttc ggcaccacca actggatggg gcaatggagc
     2161 cccgacccgg cgggcacaat ttctggcggc ggcagggccg gcgaaatacg ccggcaccct
     2221 ctggctggaa acagagtctc cctgcgaccc ccttaacccg gcctacgtct ccgcagacac
     2281 ccaggagccg ctcaactaca tccccgtcta ccacaacttt ctggaatatg tcatgcccac
     2341 cgtgctggag aacccagagg ccttttccct gaccccagcc ggacgccccc aagctattgg
     2401 tcccccgcag gatgaccaag agaggaggag aagaaccctg gccagtgtag cttccgcccg
     2461 cctcagcgcc gccgccgccg actcctactg ggacacctgg cccgacgtgg agtccaacgc
     2521 cggggagctg cttcgggaat acgtctctgc gcccaaagcc ctgatggaag acctggcaga
     2581 caaccccatt gtggccatga cacttttagc gcacgccagt ctcatcgcct ctaggaatca
     2641 ccccccttat ccagccccgg ccacagaccg ggaagttatt ctattggaac aacgggagat
     2701 gatggcgctg ctggtcggca cacatccggc ctacgctgcc gccttcctgg gcgccccatc
     2761 gttctacgca ggactcggcc tggtctcggc tctagcgaga gatgggggtt tgggagacct
     2821 cctgtcggac tcggtcctca cctacaggct ggtcaggagt ccagcctccg ggaggggggg
     2881 catgccctca accacccgcg gcagcaacga cggggaagac gcgcgccgcc tcacccgcca
     2941 ccgcatcgcc ggtccaccca ccggcttcat cttcttccag gacgcctggg aggagatgga
     3001 tacccgagcc gccctctggc cgcaccccga gttcttgggc ctggtccaca accaaagtac
     3061 ggcccgcgcc cgagcctgca tgctgctcct cgcacgccgg tgcttcgccc ccgaggccct
     3121 tcagcagctc tggcactcat tgcgcccgct ggagggcccg gtcgcatttc aggactacct
     3181 gcgcgacttt gtgaagcagg cctatacacg cggagaagag ctccccaggg cggagggact
     3241 cgaggtcccc cgcgaaaccc cgtcttccta cggcaccgtt accggccggg ccctgagaaa
     3301 tcttatgcct tatggcactc ccattaccgg tcctaagagg ggctccggtg acaccatccc
     3361 cgtctccgtt tttgaggcgg ccgtggcggc ggccttcctc ggtcggcccc taactctctt
     3421 tgtctcctct caatatctgt ttaacctgaa gaccctggga caggtcaggg tcgttgctcc
     3481 cctcctctac tgcgacgggc actctgagcc cttccggtcc ctggtggaga ccatctcgct
     3541 gaattttctg caggacctgg acggctactc cgagtctttc gagccagaga tgtccatctt
     3601 tgcacgccag gccgtgtggc tacgcgagct tctcaccgag gccagggccg ccaaacctaa
     3661 ggaggccagg ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     3721 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     3781 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     3841 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     3901 gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac
     3961 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     4021 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     4081 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     4141 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     4201 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag
     4261 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     4321 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     4381 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     4441 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     4501 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     4561 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     4621 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     4681 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     4741 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     4801 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     4861 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     4921 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     4981 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     5041 caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     5101 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5161 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5221 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5281 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5341 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5401 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5461 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5521 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5581 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5641 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5701 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5761 ggccttttgc tggccttttg ctcacatgtt
//
