LOCUS       sequence-391862-        6171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BALF2
FEATURES             Location/Qualifiers
     source          1..6171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4050..4149)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4166..4185)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4167..4185)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4190..4206)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4190..4206)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4637..5428
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5521..6109
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6010..6029
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg cagggtgcac agactagcga ggataatctg ggcagccaga gccagccggg
      721 tccgtgcggc tacatctact tttaccccct ggccacctac cctcttaggg aggtggccac
      781 actggggacc ggctacgcgg gccacaggtg cctgacggtg ccgctccttt gcggcatcac
      841 cgtggagccg ggcttcagca tcaatgtcaa ggctctgcac aggaggcccg accccaactg
      901 cgggctccta cgcgctacct cctatcacag ggacatctac gtgttccaca atgcccatat
      961 ggttcccccc atctttgagg ggccgggtct cgaggccctc tgtggcgaga ccagggaggt
     1021 gtttgggtac gacgcctaca gcgccctacc gagggaaagc tccaagccgg gggacttctt
     1081 ccccgaaggg ctagatccct ctgcctacct gggggcggtg gcaataaccg aggccttcaa
     1141 ggagcgactc tacagcggaa acctggtggc cattccatcg ttaaaacagg aggtagcggt
     1201 ggggcagtct gcgagcgtta gggtcccgct ctacgacaag gaggtgttcc cagagggcgt
     1261 gccccagctc cgccagtttt acaactcgga cctcagccgc tgcatgcacg aggcgctgta
     1321 caccgggctg gcgcaggcgc tgcgcgtccg acgggtgggc aagctggtgg agctgctgga
     1381 gaagcagagc ctgcaggacc aggccaaggt ggccaaggtg gcccccctca aggagttccc
     1441 agcctcaacc atcagtcacc cggactcggg agccttaatg attgtggaca gcgcggcatg
     1501 cgagctggcg gtgagctacg cacccgccat gctggaggcc tcgcacgaga ccccggccag
     1561 cctcaactac gactcgtggc ccctgtttgc cgactgtgag ggtccagagg cccgtgtggc
     1621 tgcgttacac cgatataatg ccagcctggc cccccacgtg tccacgcaga tctttgccac
     1681 caattccgtc ctctacgtct cgggggtctc gaagtcaacc ggtcagggca aggagagtct
     1741 ctttaacagt ttctacatga cccacggcct ggggaccctg caggagggga cctgggaccc
     1801 ctgccgccga ccctgcttct cgggctgggg tgggccagac gtgaccggaa ccaacggtcc
     1861 gggaaactac gctgtggagc acctggtcta tgcggcctcc ttctcgccca accttcttgc
     1921 ccgctatgcc tactacctgc agttttgcca gggacagaag agctctctga ccccggtgcc
     1981 ggagacgggc agctacgtgg cgggggcggc cgccagtccc atgtgctcgc tctgcgaggg
     2041 ccgggccccg gccgtgtgcc tgaacacgct cttctttagg ctgagggacc gcttcccccc
     2101 cgtcatgtcc acgcagcgga gggaccccta tgtgatctcg ggggcctcgg gctcctacaa
     2161 cgagacggac tttttgggca actttctcaa cttcatcgat aaggaggacg acgggcagcg
     2221 gccggacgac gagccccgct acacctactg gcagctgaac cagaacctgc tggagcggct
     2281 gtctcggctg ggcatagacg ctgaaggaaa gctagagaag gagccccatg gcccgcgtga
     2341 ctttgtcaag atgttcaagg acgtggatgc ggcggtggac gccgaagtgg tccagtttat
     2401 gaacagcatg gccaagaaca acatcaccta caaggacctg gtcaagagct gctaccacgt
     2461 gatgcagtac tcgtgcaacc cctttgcgca gcccgcctgc cccatcttca cccagctgtt
     2521 ttaccgctca ctgctgacca tcctgcagga catctccctg cccatctgta tgtgctatga
     2581 gaatgacaac cccgggcttg gccagagccc cccagagtgg ctaaagggtc actaccagac
     2641 gctgtgcacc aactttagga gcctggccat cgacaagggg gtcctcacgg ccaaggaggc
     2701 caaggtggtg catggggagc ccacctgcga cctgccagac ctggacgcgg ccctgcaggg
     2761 ccgggtgtac ggccggcggc tgcctgtgcg catgtccaag gtgctgatgc tgtgccccag
     2821 gaacatcaag atcaagaaca gggtggtctt cacgggggag aatgccgccc tccagaacag
     2881 cttcatcaag tccactacca ggagggagaa ctacatcatc aacgggccct acatgaaatt
     2941 cctcaacacc taccacaaga ccctattccc ggacactaag ctctcaagcc tgtacctgtg
     3001 gcacaacttt tccaggcggc gctcggtccc tgtccccagc ggggccagcg cggaggagta
     3061 ctctgacctg gccctctttg tggacggggg ctcccgggcc cacgaagaga gcaacgtcat
     3121 agatgtggtg cctggcaacc tggtcactta cgccaagcag aggctcaaca acgccatcct
     3181 gaaggcgtgc ggccagaccc agttctacat cagcctgatt cagggactgg tgccgaggac
     3241 gcagtcggtg cccgcccgtg actaccccca cgtactgggc acgcgggcgg tggagtcggc
     3301 agcggcctac gcggaggcca cctcctccct tactgcgacc acggtggtct gcgcggccac
     3361 agactgtctt agccaggtct gcaaggcccg tccggttgtc acgctgccag tgaccatcaa
     3421 caagtacacg ggggtcaacg gcaacaacca gatattccag gccgggaacc tgggatactt
     3481 tatgggccgg ggcgtggaca ggaacctgct gcaggccccc ggggctgggc tgcgcaagca
     3541 ggccgggggc tcttccatgc ggaagaagtt tgtctttgcc acccccaccc tagggttgac
     3601 cgtgaagcgc cggacccaag ccgcgaccac atatgagatt gagaacatca gggctggcct
     3661 ggaggccatt atatcacaaa aacaggagga agactgtgtg tttgatgtgg tgtgcaacct
     3721 tgtggatgcc atgggcgagg catgcgcctc gctgactagg gacgacgcgg agtacttatt
     3781 gggccgcttc tccgtcctgg cggacagcgt cctagaaacc ctggcgacca ttgcctccag
     3841 cgggatagag tggacggcgg aggccgctcg ggactttctg gagggagtgt ggggtgggcc
     3901 cggggcagcc caggacaact ttatcagcgt ggccgagccg gtcagcaccg cgtcgcaggc
     3961 ctcggccggg ctgctgctgg gtggaggagg gcagggctcc gggggcagac gcaagcgccg
     4021 tctggccacc gttctccccg gactcgaggt cccaactttc ttgtacaaag ttggcattat
     4081 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     4141 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     4201 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     4261 tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctacc
     4321 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
     4381 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     4441 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     4501 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     4561 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     4621 aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     4681 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     4741 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     4801 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     4861 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     4921 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     4981 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     5041 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     5101 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     5161 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     5221 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     5281 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     5341 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     5401 cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa
     5461 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5521 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5581 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5641 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5701 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5761 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5821 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5881 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5941 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6001 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6061 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6121 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
