LOCUS       sequence-391865-        6930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BcLF1
FEATURES             Location/Qualifiers
     source          1..6930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4809..4908)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4925..4944)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4926..4944)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4949..4965)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4949..4965)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5396..6187
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6280..6868
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6769..6788
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gcctcaaatg agggtgtgga aaacagaccc ttcccctatc tgacggtgga
      721 tgccgacctg ctctcgaacc tgcggcagtc agcggctgag gggttgtttc atagctttga
      781 cctgctggtg ggcaaggatg ccagagaggc gggcatcaag tttgaggtgc tactcggggt
      841 ctacacgaac gccatccaat atgttcgctt cctggagacg gcactggccg tgtcctgtgt
      901 gaacacggaa ttcaaagacc tgagtcgtat gacggatggc aagattcaat ttcgaatctc
      961 cgtccccacc attgctcacg gggacggaag gagacccagc aagcagcgga cattcattgt
     1021 ggtcaaaaat tgccacaaac accacattag tacggaaatg gaactgtcca tgctggatct
     1081 ggagatcctg catagtatcc ccgagacccc ggtcgagtac gcagagtacg tgggggctgt
     1141 caagaccgtg gcctcggccc tacagtttgg ggtcgatgcc ctggagaggg gcctcattaa
     1201 caccgtcctg agtgtgaagc ttcgccatgc ccctcccatg tttatcctgc agaccctggc
     1261 ggatcccacc ttcactgaga gggggttctc caagactgtc aagtctgacc tcattgccat
     1321 gttcaagagg catctgctgg agcactcctt cttcctggac cgggccgaga acatgggctc
     1381 cgggttttct cagtacgtgc gaagccgtct ctctgagatg gtagcggccg tgtccgggga
     1441 gagcgtgctc aagggggtca gtacctacac gaccgccaag gggggagagc cagtgggggg
     1501 ggtgtttatt gtcaccgaca acgttctgcg ccagctcttg accttcctgg gtgaggaggc
     1561 cgacaaccag attatggggc cctcgagcta tgcttctttt gtggtacggg gggagaacct
     1621 ggtcactgcc gtgagctacg ggcgcgtgat gcgtacgttt gagcatttca tggctaggat
     1681 cgtggactca ccggaaaaag ccggaagcac caagtctgac ctgccggctg tggccgcagg
     1741 ggtcgaggat cagccccggg tgccgatctc agccgccgtc atcaagctgg gcaaccacgc
     1801 ggtggccgtg gaaagcctgc aaaagatgta caatgacact cagtccccat accccctcaa
     1861 cagaagaatg cagtacagct actatttccc agtgggcctg tttatgccca atcccaagta
     1921 cacgacctcc gccgccatca aaatgctgga caaccccaca cagcagctac cggtggaggc
     1981 atggatcgta aacaagaata accttctcct cgcgtttaac ctacagaatg ctctcaaggt
     2041 tctctgtcac ccccgactcc acacacccgc ccatacccta aacagcctca acgcggcccc
     2101 ggccccgcga gacaggcgcg agacctactc cctgcaacac aggaggccca atcacatgaa
     2161 tgtgcttgtt attgtggacg agttctatga taacaagtat gcagcccccg tgacagatat
     2221 agctctcaag tgcgggctgc ccaccgaaga cttcctccac ccgtccaatt atgacctgct
     2281 gcggctggag ctgcaccccc tttatgatat ttacattggc agggatgccg gggagagggc
     2341 caggcacagg gctgtgcacc ggctaatggt gggtaacctg ccgactcccc tggccccagc
     2401 tgcattccaa gaggcccggg ggcagcagtt tgagaccgcc acatctctgg cccacgtggt
     2461 ggatcaggcc gttattgaga ctgtgcagga tactgcctat gacactgcct atccagcctt
     2521 cttctacgta gtcgaggcta tgatccacgg gtttgaggaa aagtttgtca tgaacgtgcc
     2581 tttggtgtcc ctgtgcatca acacctactg ggaacggtca gggaggcttg cctttgtgaa
     2641 cagcttttcc atgatcaagt tcatctgccg ccacctggga aataacgcca tctccaagga
     2701 ggcctattcc atgtatagaa aaatctatgg ggaacttata gccctagagc aggccctgat
     2761 gcgcctggcc gggtcagatg ttgtggggga tgagagcgtg ggtcagtatg tctgcgctct
     2821 cctggaccct aacctgctcc ccccggtggc ctacacagac attttcaccc atcttctcac
     2881 cgttagtgac cgggcccccc agattattat cggaaatgag gtttacgctg acaccctggc
     2941 cgcgccccag tttattgaga gggttggaaa catggatgag atggctgccc aatttgtggc
     3001 cttgtacggc taccgggtta acggagacca cgaccacgat ttccgtctgc acctaggccc
     3061 ttatgtagat gaggggcatg cggatgtgct ggaaaagatc ttttactacg ttttcctccc
     3121 aacctgcacc aatgcccaca tgtgcggcct cggggtggac tttcagcacg tggcccagac
     3181 cctggcctac aacgggccag ccttcagcca ccattttacc agggacgagg acatcctcga
     3241 caatttggag aatgggacgc tcagggatct gctggagatc tccgacctcc gccccaccgt
     3301 gggcatgatc agggacctca gcgcctcatt catgacctgc cccactttca cccgtgccgt
     3361 gcgtgtgtcg gtggacaatg acgttacgca gcagctggcc ccgaatcccg ccgacaagcg
     3421 gacagagcag actgttttgg tgaacgggct ggtggccttt gccttctccg agaggacccg
     3481 ggccgtcacc cagtgtctct ttcacgccat tcctttccat atgttttacg gggacccgcg
     3541 agtggctgcc accatgcacc aggatgttgc cacctttgtt atgcgcaatc ctcagcagcg
     3601 ggccgtggaa gccttcaacc ggccagagca gctctttgca gagtaccggg agtggcaccg
     3661 ctcgcccatg ggcaaatacg cggccgaatg tcttccttcc ctcgtttcaa tcagtggaat
     3721 gaccgccatg cacatcaaga tgtcccccat ggcctatatt gcccaggcca agctcaagat
     3781 ccacccaggg gtggccatga ccgtggtcag gaccgatgag atcctctctg aaaacatatt
     3841 gtttagctcc agggcctcaa catccatgtt cattgggacc ccaaatgtta gccgccggga
     3901 ggccagggtg gacgcggtaa cctttgaggt gcatcacgag atggcctcca tcgacaccgg
     3961 gcttagttat agctcgacca tgactccggc cagggtggcg gccatcacta ctgacatggg
     4021 tatccacacc caagacttct ttagcgtctt tccggccgag gcctttggca accagcaagt
     4081 caatgactac atcaaggcca aggtgggcgc tcagcgcaat gggacgctgc ttcgggaccc
     4141 caggacatac ctggcaggta tgactaatgt taatggagct ccaggactct gccacggcca
     4201 gcaggccacc tgtgagatta tcgtaacacc ggtcacggca gacgtggctt attttcaaaa
     4261 gtccaactct ccaaggggac gggccgcctg tgtggtctcc tgtgaaaact acaatcagga
     4321 ggttgccgag gggctcatct atgaccattc tcgcccggat gccgcctatg aataccggag
     4381 cactgtgaat ccctgggcat ctcagctggg ttctctgggt gacatcatgt acaactcctc
     4441 ctatcgccag acggccgtcc cgggcctcta cagcccctgc cgggcatttt tcaacaagga
     4501 ggagcttctg cgcaacaaca ggggactcta caacatggtc aacgagtaca gccagcgact
     4561 tggagggcac ccagccacca gcaacacaga ggtgcagttt gtagtgattg ctggcactga
     4621 cgtgtttctg gagcagccct gcagctttct gcaggaggca ttccccgcac tctcagcctc
     4681 ctcccgggca ctcatcgatg agtttatgtc tgtcaaacag acccacgccc ccatccatta
     4741 cggacactat ataattgaag aggtggcgcc ggtacgaaga atattaaagt ttggaaataa
     4801 ggtggttttt ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     4861 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     4921 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     4981 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     5041 gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac
     5101 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     5161 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     5221 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     5281 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     5341 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag
     5401 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     5461 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     5521 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     5581 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     5641 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     5701 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     5761 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     5821 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     5881 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     5941 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     6001 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     6061 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     6121 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     6181 caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     6241 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6301 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6361 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6421 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6481 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6541 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6601 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6661 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6721 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6781 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6841 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6901 ggccttttgc tggccttttg ctcacatgtt
//
