LOCUS       sequence-391867-         927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Golden Gate Level 1 Position 6. Overexpression cassette of 
            Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 
            (GRF4-GIF1). Improves in vitro wheat regeneration and 
            transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 927)
  TITLE     Golden Gate Level 1 plasmids containing the wheat GRF4-GIF1 gene 
            fusion
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pL1P6 ZmUbiP:GRF-GIF:NosT
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
ORIGIN
        1 cnntngcgga cgtttnnang tactggggtg gatgcagtgg gccccactct gtgaagacaa
       61 tgtggaattc ctcgagggag gtgcagcgtg acccggtcgt gcccctctct agagataatg
      121 agcattgcat gtctaagtta taaaaaatta ccacatattt tttttgtcac acttgtttga
      181 agtgcagttt atctatcttt atacatatat ttaaacttta ctctacgaat aatataatct
      241 atagtactac aataatatca gtgttttaga gaatcatata aatgaacagt tagacatggt
      301 ctaaaggaca attgagtatt ttgacaacag gactctacag ttttatcttt ttagtgtgca
      361 tgtgttctcc tttttttttg caaatagctt cacctatata atacttcatc cattttatta
      421 gtacatccat ttagggttta gggttaatgg tttttataga ctaatttttt tagtacatct
      481 attttattct attttagcct ctaaattaag aaaactaaaa ctctatttta gtttttttat
      541 ttaataattt agatataaaa tagaataaaa taaagtgact aaaaattaaa caaataccct
      601 ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt cgagtagata atgccagcct
      661 gttaaacgcc gtcgacgagt ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg
      721 gccaagcgaa gcagacggca cggcatctct gtcgctgcct ctggacccct ctcgagagtt
      781 ccgctccacc gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca
      841 gacgtgagcc ggcacggcnn cggcctcctc ctcctctcac ggcacggcag ctanggggat
      901 tcctttccca ccgctccttc gctttcc
//
