LOCUS       sequence-391869-        5609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the AWC (ON) neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pMZ9
FEATURES             Location/Qualifiers
     source          1..5609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2667..2816
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        3354..3458
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3459..3527,3528..4319)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3677..3696)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4490..5078
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4979..4998
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5232..5249
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5366..5387
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5402..5407,5408..5425,5426..5432)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5440..5456
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5445..5467
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5464..5480
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5464..5480
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggctcaag gttctattaa ccaacagcaa
       61 gatgtttaaa agacacgaac aacaccatag tccgagaccc caaaggccca aaaaataaat
      121 gaaaacggga tgagtacaga atacggcgcc ttcgatcgct agaaatccag tgaatatgga
      181 attcatagcg atctctagca tgtcaacaac accaacaaca aacattattt tgtagcaaga
      241 catttggaat agatcatggt caagaattac aagaaggcat ggaatggtaa taatatccat
      301 taatgatcca taaatgatgc tgacagtgcc aagaattggg cgctttaaac cgaattcagt
      361 tgtccattca tcaggtgaca ttgcactata aaattattat tgagaccaca agggtgtcgg
      421 cttttcgttt ttcacaaaaa aacatcgaaa aatcgaaaaa tgacaccctt gattgtgacg
      481 agttctaaat gagccagttc tgccaattta aaattgttcg ataaaattta gtgattagaa
      541 aaactattgc tttatctgaa tttagctttt atttaaatct ggcttccctc ataaatttaa
      601 atggaagagc ttgccgaact atgccatttt ggctcggcca tatctggggt agatttacag
      661 cgcgttacgt gtcgcgtcgc ggctcatttt ttagttctaa aactacattt acttgtccgt
      721 gtggagtaca cgactttccc acgcgttgtc cggcgggcga ttgtcaatgg agcgcgaaaa
      781 attcatcaat gaggaaggcc ggaaccccgt gatcaactgg gaaatgctca aaattgttag
      841 aaagttgaga atagcagaca catttccaaa ttttttcaat cggcaatttc taactctcag
      901 acactttttg gaacgttttt ccaaatgttt caactgaaat aaaaattaat ttcagattct
      961 tgccgttcag tgatctttat cagggatgtg cggcatattc ggcaagtcaa ctttttttaa
     1021 tatttgccga gcacggaatt gaaatttccg gcaaatcggc aaattgccag aattggaatt
     1081 ttccggcaaa ttggcaaatt gccggaattg aaatttccgg caaatcggca aattgccgga
     1141 attggaattt tccggcaaat cggcaaattg ctgatttgcc gaatttgccg gaaaaactac
     1201 aattgccaaa aattttcggc aaatcgtggt tttgcacatt ttttttggaa atttcagaat
     1261 ttcaatttta atcggcaaaa ttataggctt cctttttaaa gtaagcaaat tctatgaaaa
     1321 taactaaaga aaacgggaaa aaaattcaaa aaggcacaat tttaagtgtt tccgtcttat
     1381 aaaaaaatca ctctaaacat ttccggcaaa ctgatatccg gcaaacggca aatcggcaat
     1441 ttgcaaaaaa tttccggcaa atcggcaatt caacaaattg ataaattaaa aatttccggc
     1501 aaatcgggaa acttacaatt ttccgatttt tgtcgattgc ctgccaaaac acaggtgaaa
     1561 cttatgtatt tctcaatcta tttttgccaa atcaaaaaaa tcgtcggtgg gtaaatttag
     1621 gctgtcatat ctcggttgtc ttgtgttata tgaaaaaatt gtcaccggaa aaaaataatt
     1681 tcctattctt tttctatagt tttgcaaata attgtcaatt gataccttca aaatgaaccg
     1741 agatgtacct tttttcaaag tgcagaaagt gaatctaatt aatttgaagc caaactttga
     1801 tgcatcaaaa aaactgccaa caactgctga tcttacgatg gtctccaaga aaaacgtgac
     1861 aatgtgtcga aatctttgaa atctcgtttt tgagtgagct ctttcaaaca aaagcgaaag
     1921 cttctactca ttcaatttta aaagtttgaa taatattaat agtattacgc aataatcccg
     1981 gattagactc actataaaaa gtagtccaaa gttagcagta gcacatcaag aagcgggcgc
     2041 gctctagagg atccccggga ttggccaaag gacccaaagg tatgtttcga atgatactaa
     2101 cataacatag aacattttca ggaggaccct tggctaggat gggccctaaa aagaagcgta
     2161 aagctagcat gaagctgttg tcttcaatgg aacaagcttg cgacatatgc cggttaaaaa
     2221 aactcaggtg ctcgaaggaa aagcccaagt gtgccaagtg tctgaagaac aactcggagt
     2281 gccgttattc tcccaaggct atgaggtccc cactgaccag ggcgcatctg acagaggtag
     2341 aatcaaggtt ggaaaaattg gaagatttgt ttctgctcat gtttcctcgg gaaaacctgg
     2401 acagcatttt gaacatggac tctttggacg aagtgaaagt aatgttgaaa caattatata
     2461 tgcaggacta tggtgacacg gatgctgcta tggatagctt gacccctatg gaaaatggca
     2521 tgtctgaagg gccgagaaaa cacatgaaga ctgcgacatc atcgccaaaa gtggatgaag
     2581 atggaagcca aagtcagttg agtgtatcgc cggaattccc gctgcagtct ggtggcggag
     2641 gggctcctag gggttccgga cgggctgacg cattggacga ttttgatctg gatatgctgg
     2701 gaagtgacgc cctcgatgat tttgaccttg acatgcttgg ttcggatgcc cttgatgact
     2761 ttgacctcga catgctcggc agtgacgccc ttgatgattt cgacctggac atgctgatta
     2821 actaaggtac cgaattcgct agccggccat acaagtaatc cggatgatcg acgccaacgt
     2881 cgttgaattt tcaaatttta aatactgaat atttgttttt tttcctatta tttatttatt
     2941 ctctttgtgt tttttttctt gctttctaaa aaattaattc aatccaaatc taaacatttt
     3001 tttttctctt tccgtctccc aattcgtatt ccgctcctct catctgaaca caatgtgcaa
     3061 gtttatttat cttctcgctt tcatttcatt aggacgtggg gggaattggt ggaaggggga
     3121 aacacacaaa aggatgatgg aaatgaaata aggacacaca atatgcaaca acattcaatt
     3181 cagaaatatg gaggaaggtt taaaagaaaa cataaaaata tatagaggag gaaggaaaac
     3241 tagtaaaaaa taagcaaaga aattaggcga acgatgagaa ttgtcctcgc ttgggcccaa
     3301 aaggcctttt ttttttggcg ccgacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     3361 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3421 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     3481 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     3541 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     3601 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     3661 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     3721 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     3781 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     3841 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     3901 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3961 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4021 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4081 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4141 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4201 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4261 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4321 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4381 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     4441 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4501 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4561 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4621 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4681 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4741 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4801 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4861 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4921 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4981 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5041 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5101 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5161 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5221 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     5281 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     5341 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     5401 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     5461 acacaggaaa cagctatgac catgattacg ccaagctgta agtttaaaca tgatcttact
     5521 aactaactat tctcatttaa attttcagag cttaaaaatg gctgaaatca ctcacaacga
     5581 tggatacgct aacaacttgg aaatgaaat
//
