LOCUS       sequence-391870-        8325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuron-specific expression of LynGFP reporter. Used in combination 
            with Talpha1-Dre-pA plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 8325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pCAG-rox-inv[Talpha1-iCre-pA]-rox-LynGFP
FEATURES             Location/Qualifiers
     source          1..8325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          665..1681
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1605..1627
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1694..1713
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1694..1712
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1729..1760
                     /label=rox
                     /bound_moiety="Dre recombinase"
     primer_bind     2098..2117
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2116..2171
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2217..2236
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(3269..3289)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      3292..3301
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    4410..4441
                     /label=rox
                     /bound_moiety="Dre recombinase"
     regulatory      4458..4467
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4509..4511,4512..4514,4515..5228)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4509..4511,4512..4514,4515..5225)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4554..4575)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4815..4834)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5162..5183
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5244..5261)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5250..5474
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5546..5563)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5546..5562)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5555..5577)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5703..6158)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5840..5861)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6052..6071
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6185..6289
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6290..6358,6359..7150)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6508..6527)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7321..7909
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7810..7829
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8063..8080
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8197..8218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8233..8238,8239..8256,8257..8263)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8271..8287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8276..8298
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8295..8311
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8295..8311
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccrggs ggggsggggs ggggsggggg
      541 gsggggsggg gsgrggsggr gaggkgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
      841 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
      901 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     1021 cgggggcggt gccccgcggt gsgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1081 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1141 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1201 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     1261 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     1321 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1381 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1441 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1501 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1561 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1621 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gatccttaat taataatacg actcactata gggggtcgac ccgccaccta actttaaata
     1741 attggcatta tttaaagtta tcgcgatgaa taaatgaaag ctgtcgaggg atcttcataa
     1801 gagaagaggg acagctatga ctgggagtag tcaggagagg aggaaaaatc tggctagtaa
     1861 aacatgtaag gaaaatttta gggatgttaa agaaaaaaat aacacaaaac aaaatataaa
     1921 aaaaatctaa cctcaagtca aggcttttct atggaataag gaatggacag cagggggctg
     1981 tttcatatac tgatgacctc tttatagcca acctttgttc atggcagcca gcatatgggc
     2041 atatgttgcc aaactctaaa ccaaatactc attctgatgt tttaaatgat ttgccctccc
     2101 atatgtcctt ccgagtgaga gacacaaaaa attccaacac actattgcaa tgaaaataaa
     2161 tttcctttat tagccagaag tcagatgctc aaggggcttc atgatgtccc cataattttt
     2221 ggcagaggga aaaagatctt cagtccccat cctcgagcag cctcaccatg gccccagtct
     2281 cagagtccag gtttctgatg tagttcatca caatgttcac attggtccag ccaccagcct
     2341 gcatgatttc agggatggac acaccagccc tggccatgtc cctggcagca cccactctgg
     2401 cagagtggcc agaccaggcc aggtatctct gcccagagtc atccttggca ccatagatca
     2461 ggcggtgggt ggcctcaaag atcccttcca gggcccgggt ggacagttgg gaggtggcag
     2521 aaggggcagc cacaccattc tttctgaccc ggcagaacag gtagttgttg gggtcatcag
     2581 ccacaccaga cacagagatc catctctcca ccagcttggt aacccccagg gacagggcct
     2641 tctccacacc agctgtggac accagggtct tggtcctgcc aatgtggatc agcattctcc
     2701 caccatcggt gcgggagatg tccttcactc tgattctggc aatttcggca atgcgcagca
     2761 gggtgttgta ggcaatgccc aggaaggcca ggttcctgat gtcctggcat ctgtcagagt
     2821 tctccatcag ggatctgact tggtcaaagt cagtgcgttc aaaggccagg gcctgcttgg
     2881 ctctctcccc agcatccaca ttctcctttc tgattctcct catcaccagg gacacagcat
     2941 tggagtcaga agggcgaggc aggccagatc tcctgtgcag catgttgagc tggcccaggt
     3001 gctgttggat ggtcttcaca gccaggcctc tggcttgcag gtacaggagg tagtccctca
     3061 catcctcagg ttcagcaggg aaccatttcc tgttgttcag cttgcaccag gcagcccagg
     3121 atctgcacac agacaggagc atcttccagg tgtgttcaga gaaggcctgc ctgtccctga
     3181 acatgtccat caggttcttc ctgacttcat cagaggtggc atccacaggg agggcaggca
     3241 ggttttggtg cacagtcagc aggttggaga ctttcctctt cttcttgggc accatggtgg
     3301 cgaattcgtt gctgcttcgc ggctgccggg tggatggcgg aggcgaggag gaggtgttgc
     3361 ttcttacagc gcgactctta agcggtcgat gtaagagaac ctgcggcact tgggcggatg
     3421 cgcctcctta tatacagctc ggtcaccatg gggatgggcc aaggcctttt cctgttggtc
     3481 cagacccctc cgtctgccca gagaagccgc gacaggagag tggtgattgg tgcaagcgac
     3541 atggtatgag gtaatctctc cccccttccc cccctcgcta gcatccgtag attttgcatc
     3601 cttcagacaa aggcctcgca aaagcaacta gccgagcatc cattatgggg tggagctggg
     3661 gaggtgtaga gagggaagaa agaaaacggg ggttgggtga ttatacggag cttaaacctt
     3721 aaactgtagt taggataatt taaggttttc cccaacactt aatcacagaa tttgtgttga
     3781 gattctttat ggtaaattcc agcttttcat ttatactaca ctaaatttaa atacgttccg
     3841 tcaggattca acatctctgt tcgctttaaa taaagtagca agagctatgg gaatgatggt
     3901 caaaaaggcg tgggtaaatg tttagtttta cagttgtatt attgtggtta ttattattac
     3961 tattattatt aatatttgag caaaattcag aagctcagta tgaatagacc acgcctatga
     4021 ccctcaacag cacccgggag gcagaggcag gaagacttgc agctcaaggt catcataacc
     4081 gcccttagtg gtttatgcct gtaaatccca gcattgggaa gcggaggact gcggttattt
     4141 tgtacccggc ctggatatat agtgtgatat cctgtctcaa aaaccaaaaa caaacaaaga
     4201 gagagagaga gagaaaggaa aaaagagaaa caatatacaa agtagtactt aacgctttac
     4261 tagagagctt gctttcttta gcggtagtgc tctcccatca cccctccccg ctcgccccct
     4321 tacccgaccc tacctcaccc cagagcctgt gttattttgt ccctagaaat gagccatccc
     4381 tcctaatacg gaattccgga acccttaatt aactttaaat aattggcatt atttaaagtt
     4441 aggcagccgc cctgcaggcc accatgggat gtattaaatc aaaaaggaaa gacggggccc
     4501 ggtaccatat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     4561 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     4621 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     4681 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     4741 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     4801 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     4861 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     4921 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     4981 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     5041 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     5101 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     5161 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     5221 acaagtaaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     5281 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     5341 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     5401 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     5461 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgagat ccactagttc
     5521 tagcctcgag gctagagcgg ccgccactgg ccgtcgtttt acaacgtcgt gactgggaaa
     5581 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     5641 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     5701 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     5761 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     5821 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     5881 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     5941 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     6001 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     6061 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     6121 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     6181 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6241 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6301 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6361 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6421 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6481 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6541 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6601 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6661 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6721 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6781 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6841 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6901 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6961 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7021 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7081 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7141 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7201 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7261 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7321 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7381 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7441 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7501 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7561 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7621 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7681 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7741 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7801 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7861 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7921 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7981 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8041 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     8101 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     8161 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     8221 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     8281 ataacaattt cacacaggaa acagctatga ccatgaggcg cgccg
//
