LOCUS       sequence-391873-        6504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BOLF1
FEATURES             Location/Qualifiers
     source          1..6504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4383..4482)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4499..4518)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4500..4518)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4523..4539)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4523..4539)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4970..5761
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5854..6442
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6343..6362
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gcgtccgcta tggagagtga cagcagcggc ggtagcggag gggcagacgc
      721 ccagccaccc ctggcagagg tggacggggg gctcgcccgc gtgacgcgtc agttgctgct
      781 gtctggggac gaccccgctg cccgcctgcg cgcgctgatg cctctggagc tggggatctt
      841 tggactgggg gatttggccc agccggtgct ggtgcgcgat tttttaaata ctctcaccct
      901 catgagcggc cacgcttacc cggcggcggt cctccgccac cacgcctact acctactccg
      961 ggcggcctcc ttttccaggc gcagcttcgg cctcggccat ctggaggcgg ccctggacgt
     1021 gctggcatcc tcactgcccc ccacgacagc ctctccggcc accgacgatc cgctggacgg
     1081 ctctcggctc atcgcagaga cccgggccct ggcggccgcg taccggcgca tcatcgaaga
     1141 gggcagcggg gaggtgctcg cggtctctgg cccaacggcc acctttgcct tcgtggaaga
     1201 gctggtggcc gacacttatc tggcccggtg ggacgccttc ccgcgggagg ggctgtcatt
     1261 ctacgctttt aacgcggcga aaacgacgct ggggagatgg ctggtcaccg tatacgcgga
     1321 gaccaaccgc tacccctggg cggctgcagg gcagggtcag cccacggccg cggacatcaa
     1381 ggccatggcc gtggagctgg tggagcacag tgggggcggg gccggcgggg gagagggtga
     1441 agaaagcggt ggtggtctct ttcaccgccc cgagtcactt tccagcgtgg tggcctcgct
     1501 gcccctggcc cggcggcggg cggtagagat cctgggcgtg tacgcggagg ccagcggggg
     1561 acaaacgccc cccgtcgcgg ctgtgcccgt cctggccttt gacgccgccc ggctccggct
     1621 tttggagccc tcaggggccc tcttttacga ctatgtgtac gaggccctgc tctgggacca
     1681 gacttacggc gtcccagact cggtcatcga ggccttcctg gccgggatgg cggcagagat
     1741 ggaggccctg gccgctcgtg tgcaggaggc ggcggggagc cgggcatcct tctctccagc
     1801 cgccatcgag caggtggcca cggttctgct ctcggccggg ctcaacgaga ccgtggcggg
     1861 ggactacgcc atgatgctgg cctctgtgcc ccgggtgtcc cgctcgcggt ggaggtggct
     1921 cgaggccacc gccgccctcc tagagagtct atcgggcttt gccctgcact ttttccgact
     1981 gctgcccacc gcgagcccca cgagccgctt cgcccgtgtg gcccgggcgg cctacctgcg
     2041 ggcggaggcc gaggccgtgg acagacgagc ccggcgcacc agcggcccct ctacgcccgc
     2101 cgccgccccc gccgcgacgg ctgtgggtgt gggggcggcg gcggaccctt gggacgccgt
     2161 aacccctctg cgcatcttca tcgtcccccc tcccgcggcc gagtatgagc aggtggcggg
     2221 ggacctttca tccgaactcc tcaggtctct gctatgggtc cgctacagcc gcctgtggca
     2281 ggccccggcc ccggctccgg ccctcccctg taagcccccc ctactccccg gcgagcaggg
     2341 aagaaggcag tggacggcag cggtggcggc ggccccgcgg acagacgtcg aggcatactg
     2401 ccggtccctg agggccggcc aaacggcgcg cgcggatccg gcctatgtcc acagcccctt
     2461 cttcccggcc gccttcatcg agttccaaat ctggcccgcc cttcgccggg tcctctccaa
     2521 cgagctgccc aaaacccgct ctctggccgc cctgcgctgg ctggtctcct tcggcagcga
     2581 cctggccctc ccttcccccg agctgacccg ggcccgccgt cctctcgagc tcatatacgc
     2641 caccgtctgg gagatctatg acggggcccc tccgatgcct ggggagtccc cccaggcggt
     2701 cggactgcgc cccctcaact tagaggggga aggcaaggcc ggggacgcag gagccgaggg
     2761 tgcagaagac gaagagggcg ggggcccctg ggggctgtcg tcccacgacg cagtccttag
     2821 gatcatggac gccgtccgag aggtctcagg catcatctcc gagactatct cagcttcaga
     2881 gcgggcggcg gaagcaccac cgcttgcctg gcccacctcc ctcttttcac tcctcttcac
     2941 cctgagatac agcacgacgg cggagtcgtt gggcctggcc acccgccgct ttctggtctc
     3001 gggtgagacc ctctcggagg acatctcgcg cttgacgggg gcagcttgga ggctgtgctc
     3061 ccgcccgctc ctgtacgacg cagagaccgg gagggtgcag atccctctgg cgacggaaga
     3121 ggaggaggag gcggtggtag cggtgaagga aaagagcgtt tcatcctccc cccgccacta
     3181 ctccacggac ctccagacgc taaagagcgt cgtggagggc atccaggatg tgtgccggga
     3241 cgccgccgcc aggtgggccc tggccacggc cgacacggcc accctcagaa ggcgcctcct
     3301 ggtgccggcc ctcagggaga gccggggcat cgcggatcac cccctctggg cccacaccag
     3361 cgagcccctg cgtccggatc tcgaggagct caacgagcgg gtggagcacg ccctggagct
     3421 gggatacagc ctgacgggcg ccctcaggcg cagcgtcgcc taccggttcc gggattacac
     3481 attcgcccgc ctgttccagc cacccgccat cgacgccgag cgtgccgagg ccatagtccg
     3541 gcgagacgcc cgcccaccac ctgtcttcat ccccgcaccc aggcggcttc cgcagggggg
     3601 agcagacacc ccacctcccc tcagcatgga cgacatcctg tatctaggca agagcatctg
     3661 caaggccctg gtggacgtcc tcgaccatca tcctgccgcg ccagagacca cccctattaa
     3721 aacatacaca cccgccatgg acctaaatcc ggaacagatc acagtcaccc ccagaagccc
     3781 ctcggttctc gccgcctttg ctcgcacggc ccgggtccag acccaccacc tcgtgccggc
     3841 cctaaccgac gattccccct cacccgtggg acaaacgccc ccgccattcc gcatcctccc
     3901 cgccaaaaaa ctcgcggcca ttctcctggg taacggcagg aacgcgagca agcgccgggc
     3961 cagccgggac ctgtcaccac cgccccacgg caggtggcgt gccgttttgg actcctcccc
     4021 attctccttc tcctcctcag acttttccga ccaggacgag ggagagggag gggaggcaga
     4081 tctcaggggc gtgccgggag gaggaggaga aggagcatac gaggaagaca gggaaaggcc
     4141 atcggatatc gacaccgcgg cccgagccca gaaggttgag acctcctgcc cccgcagacg
     4201 cagtccgcgg actaccccct ctccttcaag gcgggcaagc ggcggcggcg gcccagacag
     4261 aggagaggcg gaggcacaca cgtacccccc ttatctctcg gcggctgccg ccgccagccg
     4321 cgtaagaccc cggaccagaa ggggggcgac aaggcgtcct ccccgcccca ccgccgaaga
     4381 tgagccaact ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     4441 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     4501 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     4561 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     4621 ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg
     4681 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     4741 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     4801 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     4861 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     4921 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc
     4981 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     5041 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     5101 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     5161 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     5221 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     5281 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     5341 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     5401 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     5461 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     5521 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     5581 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     5641 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     5701 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     5761 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     5821 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5881 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5941 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6001 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6061 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6121 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6181 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6241 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6301 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6361 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6421 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6481 ttgctggcct tttgctcaca tgtt
//
