LOCUS       sequence-391874-        5790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BPLF1_501-1500
FEATURES             Location/Qualifiers
     source          1..5790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3669..3768)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3785..3804)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3786..3804)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3809..3825)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3809..3825)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4256..5047
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5140..5728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5629..5648
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg caaataccca tccctctgcc acaggcagct ccctcgaacc caaaaatccc
      721 tctgaccacc ccatcccctt ccccgactgc agcagcagcc cccaccacca cgactctttc
      781 tcctcctcca actcagcagc agcccccgca atcagccgcc ccggctcctt ctccacttct
      841 tcctcagcag cagcccacgc catcagccgc cccggctcct tctccacttc ttcctcagca
      901 gcagcccccg ccatcagccg cccgggctcc ttctcctctt cctcctcagc agcagcccct
      961 gccatcagct accccggctc ctcctcctgc tcagcagctc ccaccatcag ccacgacact
     1021 cgaaccagaa aaaaaccatc ccccggccgc tgacagggct ggaacggaga tatccccatc
     1081 acctcccttc gggcagcagc cctcctttgg ggatgatgca tctgggggtt ctggcctcgt
     1141 ccgctacctg tctgacctag aggagccttt cctatccatg tccgactctg aagaagcgga
     1201 gagcgactta gcctccgata tccccaccac agaggatgaa gacatgttcg aagatgaagt
     1261 gttctccaac agcctggaat cgggatccag tgcccccacg tcccccatca cactggacac
     1321 cgccaggagc caatactatc agaccacctt tgacatagag actcctgaga tggactttgt
     1381 gcccctcgag agcaacattg cgagaatagc cggacatacc taccaggaac aggccatcgt
     1441 ctacgacccg gcctctaacc gggaagtgcc agaggcagat gccttgagca tgatagacta
     1501 tcttcttgtg acagtggtcc tcgagcaggg gctaataagg agtagggacc gatcgtctgt
     1561 gctaaatctg ctggaattct taaaggactg gtcgggacat ctgcaggtcc ccacgctgga
     1621 cctggagcaa ctgctcactt ccgaacttaa catccaaaac ctcgcaaaca tgctatcaga
     1681 aaacaaggga agagcgggag agttccataa gcacctggcc gcaaagctgg aggcctgcct
     1741 gccgtccctg gccacgaagg acgcggtccg tgtagatgcc ggtgctaaga tgctggctga
     1801 aattccccag ctggccgaga gcgacgatgg aaaatttgac ctggaggcgg cccgcagacg
     1861 cctgaccgac ctgctctcgg gcggagatca ggaggcgggg gaaggaggag gagaacccga
     1921 ggataattcc atctacaggg gcccgcacgt ggacgtcccc ttggtcctgg acgacgaaag
     1981 ctggaagcgc ctcctatccc tggcggaggc cgcccgaacg gccgtggcca gacaacaggc
     2041 tggggtagat gaggaggacg tccgcttttt ggcgctgttg acagcgatag agtatggggc
     2101 ccctcccgcc gcctcagtgc ccccctttgt ccacaacgtg gccgtccggt ccaagaatgc
     2161 ggccctgcac gtgcgcaggt gtacggcgga catcagggac aaggtggcct cggcggcctc
     2221 cgattatctc tcctaccttg aggacccaag cctcccaaca gtgatggatt ttgacgacct
     2281 cctgacacac ctgcggcaca catgccagat catcgcctcc ctgcctctcc taaacatccg
     2341 gtacacgtcc attgaatggg actatcgcga gctcctctac ctgggcaccg cccttagtga
     2401 tatgagcggt atcccctggc ccctggagcg agttgaggag gacgacccca gcattgcccc
     2461 ccttccagag tttgagacag tcgcgaaaaa acagaaggag ctcgagacca ccagagaaaa
     2521 cgaaaagcgc ctccgcacca tcctggacga catcgaggca atgttgggcc tggccggtgt
     2581 ggcctcggcc ccaggcgccc ccatctctcc cgcctctccc tctgcgacac ccgccaacca
     2641 cgacaacccc gaagccacgc caccgctggc cgacaccgcg gccctcacca tccccgtcat
     2701 agaaaagtac atcgcaaatg cagggtcgat agtcggggcc gcaaagaacc ccacgtacat
     2761 ccgcctacgt gacactatac agcagattgt tcgctccaaa aagtacctga tgaacatcct
     2821 gaaatccatc accttctaca ccatcgataa ctacattgcc tccttcgagg agagcataga
     2881 ccacctctat cgtgacttgc cggtccttga ccctgaggtg caggatggca ttgaccgcat
     2941 cctggacccc atggtctcag aggccctgca cacattcgag atgggcaacc ggctgacgct
     3001 ggagccagcg cgcctagtgg ccttgcagaa ttttgctact cacagcacct taaaggagac
     3061 ggccgcggcg gtgaatctgc tcccaggtct actggcggtc tatgatgcga cgatcacagg
     3121 tcaggctcca gaggatgccc tgagactact ctctgggctg cagaatcagc tctcccaaac
     3181 gctcatccca gggaagctca agaaacgctt cctaagttac ctgcagaaat tgaagaataa
     3241 caacaacgat cagctcagac agaaggaggt gcaggcctgg cgcctggagg ccgaaggctt
     3301 taagcccgcc accgaggaac agctcgaggc ctttctggac actgccccca acaaagagct
     3361 caagcgacag tatgagaaga agctgcggca gcttatggag accgggcgca aggaaaagga
     3421 gaagctccga gaacaggaag acaaggagag gcaggagcgg cgagcgcgag aggcgaatga
     3481 ggcctgggcc agaattagga aggcgctggg ggcacgccca gagcccgctc ccacttcgcc
     3541 cgatgactgg aacaccctgc tggcctccct gctgccagac aatacggact cagccgcggc
     3601 agcagcggcg gcagtagcca gaaacacgga catcttggac tccctcacac agatccttgc
     3661 cgctatgcta ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     3721 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     3781 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     3841 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     3901 gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac
     3961 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     4021 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     4081 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     4141 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     4201 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag
     4261 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     4321 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     4381 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     4441 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     4501 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     4561 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     4621 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     4681 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     4741 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     4801 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     4861 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     4921 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     4981 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     5041 caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     5101 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5161 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5221 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5281 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5341 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5401 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5461 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5521 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5581 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5641 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5701 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5761 ggccttttgc tggccttttg ctcacatgtt
//
