LOCUS       sequence-391877-        5601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-EBNA3B
FEATURES             Location/Qualifiers
     source          1..5601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3480..3579)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3596..3615)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3597..3615)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3620..3636)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3620..3636)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4067..4858
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4951..5539
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5440..5459
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg aagaaagcgt ggctcagcag agcacagcaa gccgatgccg ggggggcatc
      721 tggctccgag gacccaccag attatggaga tcaaggtaat gtgacacagg tgggatctga
      781 gcctatttca cctgagattg gcccctttga actctctgcg gccagtgagg atgatcctca
      841 atctgggcca gtggaagaga atttagatgc cgctgcaaga gaggaagagg aacctcatga
      901 gcaggagcac aatggtggtg acgatccctt ggatgtccat actcgccagc ctagatttgt
      961 ggatgtgaac ccaacgcagg ctccagtgat ccaactagtc cacgctgtct atgattccat
     1021 gttgcaatcg gacctccggc ccctaggcag tttattcctt gagcaaaacc tgaacatcga
     1081 agaatttata tggatgtgca tgaccgtgcg tcacagatgt caggccatca gaaaaaaacc
     1141 attaccaatt gttaagcaga ggcgttggaa gctcctgtca tcttgcagat cctggcgtat
     1201 gggttaccgc acgcataacc tcaaagtaaa cagttttgag tcaggggggg acaatgtcca
     1261 cccggtcctt gtgactgcta cgctaggatg tgatgagggc acgcggcatg caacaacgta
     1321 cagtgctggc attgtacaga taccacgaat atcagaccaa aaccaaaaga tcgaaacagc
     1381 cttcctgatg gcacgtcgtg ctaggtcact ttcggcagaa agatatactt tgttctttga
     1441 tttagtatcc tccggaaaca ccctgtatgc tatatggatt gggctgggca cgaaaaaccg
     1501 agtttcattt attgagtttg taggatggtt atgcaagaag gaccacactc atatacgcga
     1561 atggttccgc cagtgcaccg ggagacccaa agcagccaag ccatggttaa gagcgcatcc
     1621 tgtcgccatt ccttatgatg atccgttaac aaacgaggag attgatctgg cctatgcccg
     1681 cgggcaggcc atgaatattg aggctcctag actgccagat gatcctataa ttgttgagga
     1741 tgacgacgaa agtgaggaaa ttgaagctga aagcgacgag gaggaagaca agagtggaat
     1801 ggaatctctt aaaaatatac cgcaaacact gccgtacaat ccaacagtat acggcaggcc
     1861 cgcggtgttt gaccgaaagt cagatgcaaa atcaaccaaa aaatgcaggg ccatagtaac
     1921 tgactttagt gtaatcaagg ccattgaaga ggaacacaga aagaagaagg cagccagaac
     1981 agagcagcca agagccacgc ctgaatccca ggcccccaca gtggtcctcc agcgaccacc
     2041 cacgcagcaa gagcctggcc ccgtcggccc actgagtgtc caggctcggc tggagccatg
     2101 gcaacctttg cctgggcccc aagtgacagc agttctactt cacgaagaat ccatgcaggg
     2161 tgtccaagta catggttcga tgctagacct tcttgaaaaa gacgatgaag tcatggagca
     2221 gagggttatg gcaaccctac tgccaccagt accacaacag ccccgggctg gcagaagagg
     2281 cccttgtgtc ttcaccggtg acctaggcat agagagtgat gagcccgctt cgacagagcc
     2341 ggttcatgat cagctactgc ctgccccagg acctgaccct cttgaaatcc aaccactaac
     2401 gtcccccacc acgtctcaac ttagcagttc ggcaccaagc tgcgcacaaa ctccatggcc
     2461 ggtggttcag ccaagtcaga ctccagatga cccaacgaaa cagtcccggc caccggaaac
     2521 agctgcccca cgccagtggc caatgcccct gcgacctatc cctatgcgcc ccttgcggat
     2581 gcagccaatc ccatttaatc atccagtggg acccactccc catcagacac ctcaagtgga
     2641 aataacacca tataagccca cttgggctca gatagggcac attccatatc agcctacacc
     2701 aacgggtcct gctaccatgc tgttgcgcca gtgggcaccc gccaccatgc agacaccacc
     2761 gagagcgccc actcccatgt caccacctga ggtgccaccc gttccccggc agaggcctcg
     2821 gggggcgccc actcccacgc cacctcctca ggtgccgccc gttccccggc agaggcctcg
     2881 gggggcgccc actcccacgc cacctcctca ggtgctgccc actcccatgc agctggcact
     2941 aagggctcct gctggtcagc aggggccgac aaagcaaatt ttgcgccaat tgttaacggg
     3001 gggcgtcaag aaagggagac catcacttaa gttacaggcc gcccttgagc gtcaagccgc
     3061 tgcgggctgg cagccttcac cagggtccgg caccagtgac aagattgtgc aggcgcctat
     3121 tttctaccca cccgttttgc agcccataca ggttatgggg caagggggtt ccccaacggc
     3181 catggccgcc tcagcggtga cacaggcacc cacggaatat accagggaaa ggaggggagt
     3241 ggggcctatg cctcccaccg atattccgcc gtctaaacga gcgaagatcg aggcctatac
     3301 agagcccgag atgccgcacg ggggggcctc gcactctccc gtcgttatct tggagaatgt
     3361 cggccagggg caacagcaga ctctggagtg cggaggaact gctaaacagg aaagggacat
     3421 gttggggctg ggggacattg cagtttcttc cccttcctct tctgaaacat cgaacgatga
     3481 gccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     3541 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     3601 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     3661 tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca
     3721 ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa
     3781 acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     3841 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     3901 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     3961 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     4021 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt
     4081 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     4141 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     4201 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     4261 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     4321 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     4381 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     4441 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     4501 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     4561 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     4621 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     4681 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     4741 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     4801 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc
     4861 ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     4921 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4981 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5041 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5101 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5161 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5221 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5281 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5341 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5401 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5461 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5521 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5581 ctggcctttt gctcacatgt t
//
