LOCUS       sequence-391879-        8397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuron-specific expression of LynGFP reporter. Used in combination 
            with Talpha1-iCre-pA plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8397)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 8397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pCAG-lox-inv[Talpha1-Dre-pA]-lox-Lyn-EGFP
FEATURES             Location/Qualifiers
     source          1..8397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          665..1681
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1605..1627
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1694..1713
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1694..1712
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1741..1774
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2112..2131
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2130..2185
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2231..2250
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(2260..3282)
                     /product="site-specific recombinase"
                     /label=Dre recombinase
                     /note="Dre is a site-specific recombinase from 
                     bacteriophage D6. Recombination occurs at rox sequences."
     CDS             complement(3289..3315)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3316..3336)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4471..4504
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      4530..4539
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4581..4583,4584..4586,4587..5300)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4581..4583,4584..4586,4587..5297)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4626..4647)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4887..4906)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5234..5255
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5316..5333)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5322..5546
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5618..5635)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5618..5634)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5627..5649)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5776..6231
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5863..5882)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6073..6094
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6257..6361
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6362..6430,6431..7222)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6580..6599)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7393..7981
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7882..7901
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8135..8152
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8269..8290
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8305..8310,8311..8328,8329..8335)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8343..8359
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8348..8370
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8367..8383
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8367..8383
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccrggs ggggsggggs ggggsgrggg
      541 gsggggsggg gsgrggcggr gaggkgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
      841 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
      901 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     1021 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1081 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1141 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1201 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     1261 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     1321 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1381 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1441 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1501 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1561 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1621 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gatccttaat taataatacg actcactata gggggtcgac ccgccaccaa tcatttaaat
     1741 ataacttcgt ataatgtatg ctatacgaag ttattcgcga tgaataaatg aaagctgtcg
     1801 agggatcttc ataagagaag agggacagct atgactggga gtagtcagga gaggaggaaa
     1861 aatctggcta gtaaaacatg taaggaaaat tttagggatg ttaaagaaaa aaataacaca
     1921 aaacaaaata taaaaaaaat ctaacctcaa gtcaaggctt ttctatggaa taaggaatgg
     1981 acagcagggg gctgtttcat atactgatga cctctttata gccaaccttt gttcatggca
     2041 gccagcatat gggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa
     2101 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt
     2161 gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg
     2221 tccccataat ttttggcaga gggaaaaaga tctgactcag ctatccatca gtcgagaatt
     2281 ggctcccacg gacactccgc cccttctgag ataccgcatc agtgtttcag gcttcttcca
     2341 tgtcccttcc tgcataatct cgaccataga cacctgtttc tcggccatgt ctattgcggc
     2401 acccaccctg gcggaatggc cggtccaggt cctatacctc cctttattgg gggtggcgtc
     2461 ccgtttgttc agcagcaccc aggcatcact gaaaatcttc tccatggcag gggcggtcag
     2521 gggcgtggta gtaatgcggg ctttgtttga ccggtgtata ggtgggaaga gcacagcgtc
     2581 tggatgttct ctaagcccac tgacgtccag ccagtcattg agcactgctg tggttcgccg
     2641 ggagagcact ttgtcaaggc ccgcagctgt ggtgattgtc ttagtgtgac tgatgtgcag
     2701 ggtcactgtg tctcctgtct gatccaagtc cccaaccctg atgcgtgata tttcagacat
     2761 tctcatgagg gtgttatagg ctacgaacag gaaagccctg ttgcgcaggt cgaccagccg
     2821 ctcgctcctg ctgagcagca catccagcag cttcaggtcg tcccagcgca gggggattgc
     2881 ctgtcctgtc ctctcaccct tttcggttgc ggcttctctt ctaattcttc tcatggcaag
     2941 actcactgac ttgtcgtcgg acaagggtgg cagtccgcag tgggacagaa gcatattaag
     3001 catagcatag tgcttgtcga tggtagtgga ggccaggtct gcatcgtgca actgaagaaa
     3061 gtattcgcgt gccatttcag gactaattgg aaaccatgca agctgccgag catggcacca
     3121 tctggcccag ctatgaaaga ccaacctcaa atccctcaaa gtgttaggag cgtacgcccc
     3181 ctggtcattc ataaacctca tgaagttttc agcggcctcc tgatactctt tgccgatgtt
     3241 tcgcaggaat ccaccagatg aaccactaat aatcagctca gaggatccag cgtaatctgg
     3301 aacatcgtat gggtaaacct tcctcttctt cttaggcatg gtggcgaatt cgttgctgct
     3361 tcgcggctgc cgggtggatg gcggaggcga ggaggaggtg ttgcttctta cagcgcgact
     3421 cttaagcggt cgatgtaaga gaacctgcgg cacttgggcg gatgcgcctc cttatataca
     3481 gctcggtcac catggggatg ggccaaggcc ttttcctgtt ggtccagacc cctccgtctg
     3541 cccagagaag ccgcgacagg agagtggtga ttggtgcaag cgacatggta tgaggtaatc
     3601 tctcccccct tccccccctc gctagcatcc gtagattttg catccttcag acaaaggcct
     3661 cgcaaaagca actagccgag catccattat ggggtggagc tggggagggg tagagaggga
     3721 agaaagaaaa cgggggttgg gtgattatac ggagcttaaa ccttaaactg tagttaggat
     3781 aatttaaggt tttccccaac acttaatcac agaatttgtg ttgagattct ttatggtaaa
     3841 ttccagcttt tcatttatac tacactaaat ttaaatacgt tccgtcagga ttcaacatct
     3901 ctgttcgctt taaataaagt agcaagagct atgggaatga tggtcaaaaa ggcgtgggta
     3961 aatgtttagt tttacagttg tattattgtg gttattatta ttactattat tattaatatt
     4021 tgagcaaaat tcagaagctc agtatgaata gaccacgcct atgaccctca acagcacccg
     4081 ggaggcagag gcaggaagac ttgcagctca aggtcatcat aaccgccctt agtggtttat
     4141 gcctgtaaat cccagcattg ggaagcggag gactgcggtt attttgtacc cggcctggat
     4201 atatagtgtg atatcctgtc tcaaaaacca aaaacaaaca aagagagaga gagagagaaa
     4261 ggaaaaaaga gaaacaatat acaaagtagt acttaacgct ttactagaga gcttgctttc
     4321 tttagcggta gtgctctccc atcacccctc cccgctcgcc cccttacccg accctacctc
     4381 accccagagc ctgtgttatt ttgtccctag aaatgagcca tccctcctaa tacggaatga
     4441 tccccatcaa gctgatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag
     4501 ttattaggtc cctcgacctg cagctgcagg ccaccatggg atgtattaaa tcaaaaagga
     4561 aagacggggc ccggtaccat atggtgagca agggcgagga gctgttcacc ggggtggtgc
     4621 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     4681 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     4741 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     4801 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     4861 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     4921 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     4981 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     5041 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     5101 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     5161 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     5221 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     5281 tggacgagct gtacaagtaa gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     5341 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     5401 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     5461 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     5521 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag
     5581 atccactagt tctagcctcg aggctagagc ggccgccact ggccgtcgtt ttacaacgtc
     5641 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     5701 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     5761 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5821 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5881 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5941 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6001 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6061 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6121 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6181 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     6241 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6301 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6361 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6421 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6481 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6541 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6601 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6661 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6721 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6781 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6841 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6901 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6961 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7021 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7081 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7141 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7201 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7261 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7321 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7381 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7441 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7501 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7561 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7621 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7681 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7741 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7801 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7861 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7921 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7981 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8041 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8101 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8161 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8221 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8281 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8341 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgagg cgcgccg
//
