LOCUS       sequence-391880-        8398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuron-specific expression of mOrange2 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 8398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1
FEATURES             Location/Qualifiers
     source          1..8398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          665..1681
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1605..1627
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1694..1713
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1694..1712
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1741..1774
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2112..2131
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2130..2185
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2231..2250
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(3283..3303)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      3306..3315
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    4424..4457
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      4500..4509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4506..5213
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    5223..5279
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(5317..5334)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5323..5547
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5619..5636)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5619..5635)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5628..5650)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5777..6232
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5864..5883)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6074..6095
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6258..6362
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6363..6431,6432..7223)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6581..6600)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7394..7982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7883..7902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8136..8153
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8270..8291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8306..8311,8312..8329,8330..8336)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8344..8360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8349..8371
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8368..8384
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8368..8384
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccrggs ggggsggggs ggggsgrggg
      541 gsggggsggg gsgrggsggr gaggkgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
      841 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
      901 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     1021 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1081 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1141 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1201 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     1261 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     1321 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1381 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1441 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1501 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1561 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1621 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gatccttaat taataatacg actcactata gggggtcgac ccgccaccaa tcatttaaat
     1741 ataacttcgt ataatgtatg ctatacgaag ttattcgcga tgaataaatg aaagctgtcg
     1801 agggatcttc ataagagaag agggacagct atgactggga gtagtcagga gaggaggaaa
     1861 aatctggcta gtaaaacatg taaggaaaat tttagggatg ttaaagaaaa aaataacaca
     1921 aaacaaaata taaaaaaaat ctaacctcaa gtcaaggctt ttctatggaa taaggaatgg
     1981 acagcagggg gctgtttcat atactgatga cctctttata gccaaccttt gttcatggca
     2041 gccagcatat gggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa
     2101 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt
     2161 gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg
     2221 tccccataat ttttggcaga gggaaaaaga tcttcagtcc ccatcctcga gcagcctcac
     2281 catggcccca gtctcagagt ccaggtttct gatgtagttc atcacaatgt tcacattggt
     2341 ccagccacca gcctgcatga tttcagggat ggacacacca gccctggcca tgtccctggc
     2401 agcacccact ctggcagagt ggccagacca ggccaggtat ctctgcccag agtcatcctt
     2461 ggcaccatag atcaggcggt gggtggcctc aaagatccct tccagggccc gggtggacag
     2521 ttgggaggtg gcagaagggg cagccacacc attctttctg acccggcaga acaggtagtt
     2581 gttggggtca tcagccacac cagacacaga gatccatctc tccaccagct tggtaacccc
     2641 cagggacagg gccttctcca caccagctgt ggacaccagg gtcttggtcc tgccaatgtg
     2701 gatcagcatt ctcccaccat cggtgcggga gatgtccttc actctgattc tggcaatttc
     2761 ggcaatgcgc agcagggtgt tgtaggcaat gcccaggaag gccaggttcc tgatgtcctg
     2821 gcatctgtca gagttctcca tcagggatct gacttggtca aagtcagtgc gttcaaaggc
     2881 cagggcctgc ttggctctct ccccagcatc cacattctcc tttctgattc tcctcatcac
     2941 cagggacaca gcattggagt cagaagggcg aggcaggcca gatctcctgt gcagcatgtt
     3001 gagctggccc aggtgctgtt ggatggtctt cacagccagg cctctggctt gcaggtacag
     3061 gaggtagtcc ctcacatcct caggttcagc agggaaccat ttcctgttgt tcagcttgca
     3121 ccaggcagcc caggatctgc acacagacag gagcatcttc caggtgtgtt cagagaaggc
     3181 ctgcctgtcc ctgaacatgt ccatcaggtt cttcctgact tcatcagagg tggcatccac
     3241 agggagggca ggcaggtttt ggtgcacagt cagcaggttg gagactttcc tcttcttctt
     3301 gggcaccatg gtggcgaatt cgttgctgct tcgcggctgc cgggtggatg gcggaggcga
     3361 ggaggaggtg ttgcttctta cagcgcgact cttaagcggt cgatgtaaga gaacctgcgg
     3421 cacttgggcg gatgcgcctc cttatataca gctcggtcac catggggatg ggccaaggcc
     3481 ttttcctgtt ggtccagacc cctccgtctg cccagagaag ccgcgacagg agagtggtga
     3541 ttggtgcaag cgacatggta tgaggtaatc tctcccccct tccccccctc gctagcatcc
     3601 gtagattttg catccttcag acaaaggcct cgcaaaagca actagccgag catccattat
     3661 ggggtggagc tggggagggg tagagaggga agaaagaaaa cgggggttgg gtgattatac
     3721 ggagcttaaa ccttaaactg tagttaggat aatttaaggt tttccccaac acttaatcac
     3781 agaatttgtg ttgagattct ttatggtaaa ttccagcttt tcatttatac tacactaaat
     3841 ttaaatacgt tccgtcagga ttcaacatct ctgttcgctt taaataaagt agcaagagct
     3901 atgggaatga tggtcaaaaa ggcgtgggta aatgtttagt tttacagttg tattattgtg
     3961 gttattatta ttactattat tattaatatt tgagcaaaat tcagaagctc agtatgaata
     4021 gaccacgcct atgaccctca acagcacccg ggaggcagag gcaggaagac ttgcagctca
     4081 aggtcatcat aaccgccctt agtggtttat gcctgtaaat cccagcattg ggaagcggag
     4141 gactgcggtt attttgtacc cggcctggat atatagtgtg atatcctgtc tcaaaaacca
     4201 aaaacaaaca aagagagaga gagagagaaa ggaaaaaaga gaaacaatat acaaagtagt
     4261 acttaacgct ttactagaga gcttgctttc tttagcggta gtgctctccc atcacccctc
     4321 cccgctcgcc cccttacccg accctacctc accccagagc ctgtgttatt ttgtccctag
     4381 aaatgagcca tccctcctaa tacggaattc cggaaccctt aatataactt cgtataatgt
     4441 atgctatacg aagttattag gtccctcgac ctgcagcctc cgcagccccc aagctagcag
     4501 ccaccatggt gagcaagggc gaggagaata acatggccat catcaaggag ttcatgcgct
     4561 tcaaggtgcg catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     4621 agggccgccc ctacgagggc tttcagaccg ctaagctgaa ggtgaccaag ggtggccccc
     4681 tgcccttcgc ctgggacatc ctgtcccctc atttcaccta cggctccaag gcctacgtga
     4741 agcaccccgc cgacatcccc gactacttca agctgtcctt ccccgagggc ttcaagtggg
     4801 agcgcgtgat gaactacgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     4861 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     4921 ccgtgatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     4981 acggtgccct gaagggcaag atcaagatga ggctgaagct gaaggacggc ggccactaca
     5041 cctccgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     5101 tcgtcgacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     5161 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtcaggac
     5221 tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca
     5281 ccggatctag ataagtaatg agagctcgct gatcagcctc gactgtgcct tctagttgcc
     5341 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     5401 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     5461 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     5521 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga
     5581 gatccactag ttctagcctc gaggctagag cggccgccac tggccgtcgt tttacaacgt
     5641 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5701 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5761 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5821 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5881 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5941 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6001 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6061 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6121 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6181 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     6241 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6301 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6361 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6421 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6481 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6541 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6601 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6661 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6721 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6781 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6841 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6901 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6961 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7021 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7081 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7141 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7201 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7261 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7321 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7381 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7441 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7501 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7561 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7621 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7681 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7741 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7801 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7861 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7921 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7981 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8041 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8101 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8161 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8221 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     8281 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     8341 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgag gcgcgccg
//
