LOCUS       sequence-391888-        6364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression plasmid for the OLL neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP172
FEATURES             Location/Qualifiers
     source          1..6364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3422..3571
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        4109..4213
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4214..4282,4283..5074)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4432..4451)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5245..5833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5734..5753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5987..6004
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6121..6142
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6157..6162,6163..6180,6181..6187)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6195..6211
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6200..6222
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6219..6235
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6219..6235
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccccag ttcaaattaa ctctacccaa
       61 ctacacatca caagtgctat atagttatat agaaatcgta aggtgtgaga aattaagcaa
      121 ttaaaaccaa agtcaagttg attttttgtt gaaatttgag tcaaccggcg ctgtttgtat
      181 acacatatac aaacaacggt gactcctatt ttttttcgat aacctttgat tcttcaaatt
      241 gttttgttac gcaacaatta aaggatcgta tgatcgtact caattggttg taaaacatgt
      301 ttaaaacttc attttactgt gttttgactt cactttttcc attttctttt cttgtttttt
      361 tggcaactac cgtactcttt gctatttact aagaagttaa ttatggagtg acgggatatg
      421 ttttaggtgt tcgtgtgttg tttcgatttc tcatccatag ctcaaagtgt cacaaacaac
      481 ggttagcaca tgctagaatt ttcctttttt tgttgttttg ttctttctct cttaaaatca
      541 tggtccattg aaatagtatg tgcaatcgaa cgccgtaagt gagaagaaaa cagaagcaaa
      601 tgagtgttct ataaatagag aaattgaata gatttctata aaaactagaa gattaagttc
      661 cccccttctc tctttctttt tcacttttta tttcaaaagt ggttcaacta attatctggg
      721 aaaaaacaac tagaaaaaga aaaattggca caaaaacaga ggaccaataa caaatcaaag
      781 tgtgcatgaa attttgaaaa tctgcaaatg tgcactttat tttaacgtaa cagggaaggt
      841 gacattaaat ttgaaaagtt aagttattta acacctattg agaaaatatg gtacttaagt
      901 gagaattgaa gcggacgctt ttaagggcct aattcttttg actacgatgc atcaacagta
      961 cactcaattt ctaaaataat ggaaatatta tcttcaagat gaaaaaaaac acccaatttt
     1021 cttatgattt gaattaaaaa ttgtgaggtt tttccttttt cgtataattt tcaaacatgt
     1081 caactaagaa ttctggctta agaaacgcca atatggcaaa atatcgtaac agtaattaat
     1141 taaacaccat gaatacattt taacatttct tgtaaaattg tttcattaaa gttaaaagtg
     1201 caattactta cttttcgcat ttcataagtt cataaaaaag aaggcaacta aaatttgttg
     1261 gttttctaag acgttcctat ttaaaggttg agtagcgtca gtggggattt tgtctaaatc
     1321 taaatctaaa tctatcgtga ttcaaaacga ccaaatatca taataaaaca ctccaaaaat
     1381 gatttttcat aatttccgaa gtttttgcaa actgccaaaa ttttgaaaaa tatgagcttt
     1441 tgaggaattt caaagaaatg ccacatgttc cgacccctac aatgttttaa tacaaataat
     1501 taaaacaaaa ttacagtata aaaagtgtag aaaatttttt ttttcaggtc gacttccaaa
     1561 attatgagtg gcaaaaactg agtaactgtc acgttttgac agtaactggc gctactccac
     1621 ctttaagtta catttcaatt tttttaatca acattgtaga ggttcaaaca tgcgatgccc
     1681 aatttttttt tcaaacggcc agttttttgt ttgaaaattt tccgaaactg acttgtttga
     1741 aaatgttcaa aagtacacgt ttttgtgatt tccgcccaca tttgactata atgagtgtat
     1801 tcaaacaaac cccctttgaa gctactcctt ctttaatgct ttaacttcat acaactccta
     1861 aacctacgtg taagctttac ccagctaatc atctattggt tgtccgatca tttgcatctg
     1921 catttccaat catttggtaa ttacacgaca tttcttcact ttaatacccg ggaatactac
     1981 aacaacaaca actttttatg caggccaatc tatcttcatt ttcctgagaa gcaacttttt
     2041 aatatcttta agaataaaca atttttttgg gggggggggg gggggcagcc gacagttggs
     2101 gtgttyttty ttatgcttgg wtttttgctc tcagcaaaag ttcattttaa ttcaaaaagt
     2161 tcgagcttgt tcggtcggct tgttcscctt cctcttcatt tcttttcaac atttcmccaa
     2221 sccaatttga gcaacttcaa ctcctttttc cccccccccc cccccccccc tccctattcc
     2281 aattggtgtt tgagcctttt ctattcgagt cttattttta ttgcgagtaa acctagaagt
     2341 attatttcca cgccgtgaaa agggactaat gaatgtagtt tcctcttttt ttttttgaag
     2401 agttcttggt cgcctctttt ttagtgggtt cttagacctt atatgacaaa aggaaaccca
     2461 tgaatcagga tctggcagtg aggcgtgtaa tcctttcttt cctcaccatt tcaaaatact
     2521 ttgaaggttc tggcaaaaac cggactctat atattccaca tcggtgctct tattctcggt
     2581 ttcatattta tttatattta tttttgctgc tgctaaatcg acttcttaca tccgtgaaac
     2641 caactttagg aatatctaga tctgttatta tgtttgtgat ttgcaagcag acatgtcaag
     2701 ttataattaa tctcagacaa tctctaaatt gttgcctaat ctcaattgcc ctttgctcgg
     2761 gcatcctaga aatctgtaag ccattggctt ttctgtgact gctctttgtt ttctcacaga
     2821 tctcaatggt gctactacta acggctcaat tttcattcag ggcgtcgggt cgtgccgcac
     2881 cattacttgt tttattcact cttttttctc tttttttttc ttcatctcac taaaataaaa
     2941 tagttttgtc cccccccccc cccctctttt gcctcttttg cccggagcat catcccctcc
     3001 ctttttcacc ccttctttgc cacccactat gaactcgacg tgttattgtc attttcagtc
     3061 cttttgcgca gatttcttct tcctacattg tcaacctttc agaaacttgt tgctcaggcg
     3121 cgcctctaga ggatccccgg gattggccaa aggacccaaa ggtatgtttc gaatgatact
     3181 aacataacat agaacatttt caggaggacc cttggctagg atgggcccta aaaagaagcg
     3241 taaagctagc atgatgttga agaagattct caaaattgaa gaactggatg agcgtgagct
     3301 catcgacatc gaggtctccg gaaaccacct cttctacgcc aacgacatcc tcacccacaa
     3361 ctcctcctcc gatgtactgc agtctggtgg cggaggggct cctaggggtt ccggacgggc
     3421 tgacgcattg gacgattttg atctggatat gctgggaagt gacgccctcg atgattttga
     3481 ccttgacatg cttggttcgg atgcccttga tgactttgac ctcgacatgc tcggcagtga
     3541 cgcccttgat gatttcgacc tggacatgct gattaactaa ggtaccgaat tcgctagccg
     3601 gccatacaag taatccggat gatcgacgcc aacgtcgttg aattttcaaa ttttaaatac
     3661 tgaatatttg ttttttttcc tattatttat ttattctctt tgtgtttttt ttcttgcttt
     3721 ctaaaaaatt aattcaatcc aaatctaaac attttttttt ctctttccgt ctcccaattc
     3781 gtattccgct cctctcatct gaacacaatg tgcaagttta tttatcttct cgctttcatt
     3841 tcattaggac gtggggggaa ttggtggaag ggggaaacac acaaaaggat gatggaaatg
     3901 aaataaggac acacaatatg caacaacatt caattcagaa atatggagga aggtttaaaa
     3961 gaaaacataa aaatatatag aggaggaagg aaaactagta aaaaataagc aaagaaatta
     4021 ggcgaacgat gagaattgtc ctcgcttggg cccaaaaggc cttttttttt tggcgccgac
     4081 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4141 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4201 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4261 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4321 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4381 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4441 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4501 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4561 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4621 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4681 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4741 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     4801 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4861 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4921 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4981 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5041 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5101 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5161 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5221 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5281 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5341 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5401 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5461 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5521 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5581 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5641 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5701 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5761 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5821 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5881 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5941 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     6001 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     6061 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     6121 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     6181 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     6241 ttacgccaag ctgtaagttt aaacatgatc ttactaacta actattctca tttaaatttt
     6301 cagagcttaa aaatggctga aatcactcac aacgatggat acgctaacaa cttggaaatg
     6361 aaat
//
