LOCUS       sequence-391893-        5790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BPLF1_1001-2000
FEATURES             Location/Qualifiers
     source          1..5790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3669..3768)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3785..3804)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3786..3804)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3809..3825)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3809..3825)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4256..5047
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5140..5728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5629..5648
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gtgcgcaggt gtacggcgga catcagggac aaggtggcct cggcggcctc
      721 cgattatctc tcctaccttg aggacccaag cctcccaaca gtgatggatt ttgacgacct
      781 cctgacacac ctgcggcaca catgccagat catcgcctcc ctgcctctcc taaacatccg
      841 gtacacgtcc attgaatggg actatcgcga gctcctctac ctgggcaccg cccttagtga
      901 tatgagcggt atcccctggc ccctggagcg agttgaggag gacgacccca gcattgcccc
      961 ccttccagag tttgagacag tcgcgaaaaa acagaaggag ctcgagacca ccagagaaaa
     1021 cgaaaagcgc ctccgcacca tcctggacga catcgaggca atgttgggcc tggccggtgt
     1081 ggcctcggcc ccaggcgccc ccatctctcc cgcctctccc tctgcgacac ccgccaacca
     1141 cgacaacccc gaagccacgc caccgctggc cgacaccgcg gccctcacca tccccgtcat
     1201 agaaaagtac atcgcaaatg cagggtcgat agtcggggcc gcaaagaacc ccacgtacat
     1261 ccgcctacgt gacactatac agcagattgt tcgctccaaa aagtacctga tgaacatcct
     1321 gaaatccatc accttctaca ccatcgataa ctacattgcc tccttcgagg agagcataga
     1381 ccacctctat cgtgacttgc cggtccttga ccctgaggtg caggatggca ttgaccgcat
     1441 cctggacccc atggtctcag aggccctgca cacattcgag atgggcaacc ggctgacgct
     1501 ggagccagcg cgcctagtgg ccttgcagaa ttttgctact cacagcacct taaaggagac
     1561 ggccgcggcg gtgaatctgc tcccaggtct actggcggtc tatgatgcga cgatcacagg
     1621 tcaggctcca gaggatgccc tgagactact ctctgggctg cagaatcagc tctcccaaac
     1681 gctcatccca gggaagctca agaaacgctt cctaagttac ctgcagaaat tgaagaataa
     1741 caacaacgat cagctcagac agaaggaggt gcaggcctgg cgcctggagg ccgaaggctt
     1801 taagcccgcc accgaggaac agctcgaggc ctttctggac actgccccca acaaagagct
     1861 caagcgacag tatgagaaga agctgcggca gcttatggag accgggcgca aggaaaagga
     1921 gaagctccga gaacaggaag acaaggagag gcaggagcgg cgagcgcgag aggcgaatga
     1981 ggcctgggcc agaattagga aggcgctggg ggcacgccca gagcccgctc ccacttcgcc
     2041 cgatgactgg aacaccctgc tggcctccct gctgccagac aatacggact cagccgcggc
     2101 agcagcggcg gcagtagcca gaaacacgga catcttggac tccctcacac agatccttgc
     2161 cgctatgcta ttgggaataa cgagggtgag gagggagaga ctgcgatccc tgctcgtgga
     2221 cgatggtggt gcggcggaga gaatggaggc cgcggaaccc ggctggttca cggatatcga
     2281 gaccggccct ctggccagac tggacgcttg gcccgccacc cccgcggcta ccgccaaaga
     2341 aggaggagga gggcgaggag cggaggaagc ggccggggcc ctctttcgcg ccaggacggc
     2401 ggccgatgcc atccgttcgg ccctcgcgca gacgcgccag gccctgcagt ccccggacat
     2461 gaaatcagcg gtggtcaaca cggatctgga ggccccctac gcggagtacg agcgggggct
     2521 ggccgggctt ctggaaaaaa gacgagcagc cgaggctgcc ctgacggcca tcgtgagcga
     2581 gtacgtggat cggacgctac ccgaagccac taatgaccca ggccaggcta acctgcctcc
     2641 tcctccaact atcccccagg caaccgcccc gcccaggctg gcctcggact cggcgctctg
     2701 gcctaagaag ccccagctgc tgacaaggcg agagcgggac gatctcctcc aggccacggg
     2761 ggacttcttc tcggagctgc tgaccgaggc cgaggcggcc gaggtccggg cgctggaaga
     2821 gcaggtccgg gagagccaga ccctgatggc gaaggcccac gagatggcgg caagcactcg
     2881 gcggggcttt cacacggctc tggaggccgt cctctccagg tcacgcgacg aagcccccga
     2941 cgatgaactc cggagcctgc ttccctcccc gcccaaagcc cctgtccagg ctcccctcga
     3001 ggccgccctg gcccgggcag cggccgggaa cggctcatgg ccctaccgga aatccctggc
     3061 agccgccaag tggatccggg gcatctgcga ggccgtgcgg ggtctttccg aaggagccct
     3121 ggccttagct gggggcgcgg gtgcctggct gaacctagcc gctgcggctg acggtgaaat
     3181 tcatgagcta acacggctcc tggaagtcga gggcatggcc cagaactcca tggatggcat
     3241 ggaggagctg aggttggctc tggccacgtt ggaccccaag cgggtcgcgg ggggcaagga
     3301 gaccgtcgca gactggaaaa ggcgcctctc ccgactggag gccatcatcc aggaggccca
     3361 ggaagagtcc cagctgcagg gaacgctgca ggatctggtc acccaggcca ggggccacac
     3421 cgacccgcgc cagctcaaga tcgtggtgga ggctgccagg ggcctggcgc ttggggcctc
     3481 ggctggctcc cagtatgccc tcctcaagga caagctgctg cgctatgcct cggccaagca
     3541 gagtttcctg gctttttacg agaccgccca gcctaccgtt ttcgttaagc atcccttgac
     3601 caacaacctg cccctcctca tcacgatttc ggcaccacca actggatggg gcaatggagc
     3661 cccgacccgg ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     3721 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     3781 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     3841 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     3901 gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac
     3961 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     4021 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     4081 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     4141 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     4201 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag
     4261 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     4321 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     4381 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     4441 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     4501 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     4561 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     4621 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     4681 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     4741 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     4801 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     4861 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     4921 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     4981 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     5041 caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     5101 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5161 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5221 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5281 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5341 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5401 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5461 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5521 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5581 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5641 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5701 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5761 ggccttttgc tggccttttg ctcacatgtt
//
