LOCUS       sequence-391894-        8918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modular CD8hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For 
            Transient Expression or Lentiviral Production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8918)
  AUTHORS   Timpanaro A, Piccand C, Dzhumashev D, Anton-Joseph S, Robbi A, Moser
            J, Rossler J, Bernasconi M
  TITLE     CD276-CAR T cells and Dual-CAR T cells targeting CD276/FGFR4 promote
            rhabdomyosarcoma clearance in orthotopic mouse models.
  JOURNAL   J Exp Clin Cancer Res. 2023 Nov 4;42(1):293. doi: 
            10.1186/s13046-023-02838-3.
  PUBMED    37924157
REFERENCE   2  (bases 1 to 8918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Clin 
            Cancer Res."; date: "2023-11-4"; volume: "42(1):293. doi"; pages: " 
            10.1186/s13046-023-02838-3"
COMMENT     Sequence Label: pLV-EF1A-CD19.8H.28TM.BB.28.3z
FEATURES             Location/Qualifiers
     source          1..8918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1965..2176
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     protein_bind    2182..2206
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2273..2302
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2303..2332
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3938..3994
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3995..3997,3998..4711)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3995..3997,3998..4708)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4037..4058)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4298..4317)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4645..4666
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4721..4745)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4763..5351
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4816..4836)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5234..5245)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5439..5672
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5744..5865
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5781..5800)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5835..5854
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5905..6040
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6060..6079)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6061..6079)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6089..6106)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6089..6105)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6098..6120)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6247..6702
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6334..6353)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6544..6565
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6728..6832
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6833..6901,6902..7693)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7051..7070)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7864..8452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8353..8372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8606..8623
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8740..8761
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8776..8781,8782..8799,8800..8806)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8814..8830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8819..8841
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8838..8854
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8838..8854
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8873..8893
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8875..8893
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgtc tagagggcag agcgcacatc
     1981 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     2041 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     2101 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     2161 tgccgccaga acacagggat cccaagtttg tacaaaaaag caggctgcca ccatgctcag
     2221 gctgctcttg gctctcaact tattcccttc aattcaagta acaggactcg aggaacaaaa
     2281 actcatctca gaagaggatc tggaacaaaa gctcatttca gaagaagatc tgctcgaggt
     2341 cgacggagac gccgacatcc agatgacaca gactacatcc tccctgtctg cctctctggg
     2401 agacagagtc accatcagtt gcagggcaag tcaggacatc tctaagtatt tgaattggta
     2461 tcagcagaaa ccagatggaa ctgttaaact cctgatctac catacatcaa gattacactc
     2521 aggagtccca tcaaggttca gtggcagtgg gtctggaaca gattattctc tcaccattag
     2581 caacctggag caagaagata ttgccactta cttttgccaa cagggtaata cgcttccgta
     2641 cacgttcgga ggggggacta agttggaaat aacaggctcc acctctggat ccggcaagcc
     2701 cggatctggc gagggatcca ccaagggcga ggtgaaactg caggagtcag gacctggcct
     2761 ggtggcgccc tcacagagcc tgtccgtcac atgcactgtc tcaggggtct cattacccga
     2821 ctatggtgta agctggattc gccagcctcc acgaaagggt ctggagtggc tgggagtaat
     2881 atggggtagt gaaaccacat actataattc agctctcaaa tccagactga ccatcatcaa
     2941 ggacaactcc aagagccaag ttttcttaaa aatgaacagt ctgcaaactg atgacacagc
     3001 catttactac tgtgccaaac attattacta cggtggtagc tatgctatgg actactgggg
     3061 tcaaggaacc tcagtcaccg tctccggtac caccacaacg cccgctcctc ggccaccgac
     3121 gccagcgcca actattgcga gtcagcctct cagtctgcga cctgaggctt gtcgaccagc
     3181 agccggaggc gcagtgcaca cgagggggct ggacttcgcc tgtgatagaa gacctccttc
     3241 taagcccttt tgggtgctgg tggtggttgg tggagtcctg gcttgctata gcttgctagt
     3301 aacagtggcc tttattattt tctgggtgag gaaacggggc agaaagaaac tcctgtatat
     3361 attcaaacaa ccatttatga gaccagtaca aactactcaa gaggaagatg gctgtagctg
     3421 ccgatttcca gaagaagaag aaggaggatg tgaactgagt aagaggagca ggctcctgca
     3481 cagtgactac atgaacatga ctcccaggcg gcccggaccc acccgcaagc attaccagcc
     3541 ctatgcccca ccacgcgact tcgcagccta tcgctcctcc ggactgagag tgaagttcag
     3601 caggagcgca gacgcccccg cgtaccagca gggccagaac cagctctata acgagctcaa
     3661 tctaggacga agagaggagt acgatgtttt ggacaagagg cgtggccggg accctgagat
     3721 ggggggaaag ccgcagagaa ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa
     3781 agataagatg gcggaggcct acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa
     3841 ggggcacgat ggcctttacc agggtctcag tacagccacc aaggacacct acgacgccct
     3901 tcacatgcag gccctgcccc ctcgctccgg acgtacggcc acgaacttct ctctgttaaa
     3961 gcaagcaggc gacgtggaag aaaaccccgg tcccgtgagc aagggcgagg agctgttcac
     4021 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     4081 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     4141 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     4201 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     4261 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     4321 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     4381 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     4441 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     4501 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     4561 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     4621 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4681 cactctcggc atggacgagc tgtacaagta agggccccgt acccagcttt cttgtacaaa
     4741 gtggtgataa tcgaattccg ataatcaacc tctggattac aaaatttgtg aaagattgac
     4801 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     4861 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     4921 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     4981 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     5041 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     5101 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     5161 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     5221 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     5281 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     5341 cgcctccccg catcgggaat tcccgcggtt cgctttaaga ccaatgactt acaaggcagc
     5401 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     5461 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     5521 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5581 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5641 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     5701 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     5761 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5821 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     5881 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5941 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     6001 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     6061 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     6121 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     6181 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     6241 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6301 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     6361 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6421 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6481 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6541 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6601 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6661 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     6721 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6781 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6841 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6901 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6961 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7021 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     7081 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7141 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7201 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7261 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7321 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     7381 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     7441 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7501 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7561 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7621 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7681 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7741 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7801 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7861 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7921 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7981 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     8041 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8101 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8161 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8221 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8281 agagagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8341 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8401 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8461 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8521 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8581 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     8641 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     8701 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     8761 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     8821 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     8881 ccctcactaa agggaacaaa agctggagct gcaagctt
//
