LOCUS       sequence-391895-        5832 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BALF5
FEATURES             Location/Qualifiers
     source          1..5832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3711..3810)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3827..3846)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3828..3846)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3851..3867)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3851..3867)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4298..5089
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5182..5770
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5671..5690
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg tctgggggac tcttctataa ccctttccta agacctaata aaggccttct
      721 gaaaaagcct gacaaggagt acctgcgtct cattcccaag tgtttccaga caccaggcgc
      781 cgcaggggtg gtggatgtgc gggggcctca gccccccctg tgcttctacc aagactccct
      841 gacggtggtg gggggtgacg aggatggaaa gggcatgtgg tggcgccagc gtgcccaaga
      901 gggcacggca aggccggagg cagacaccca cggaagccct ctggacttcc atgtctacga
      961 catactcgag acggtgtaca cgcacgagaa atgcgccgtc attccatcgg ataaacaggg
     1021 gtatgtggtg ccatgtggca tcgtcatcaa gctactgggc cggcgcaagg ccgatggggc
     1081 cagcgtgtgt gtgaacgtgt ttgggcagca ggcctacttc tacgccagcg cgcctcaggg
     1141 tctggacgtg gagtttgcag tcctcagcgc cctcaaggcc agcaccttcg accgcaggac
     1201 cccctgccgg gtctcggtgg agaaggtcac gcgccgttcc attatgggct acggcaacca
     1261 tgccggcgac taccacaaga tcaccctctc ccatcccaac agtgtgtgtc acgtggccac
     1321 gtggctgcaa gacaagcacg ggtgtcggat ctttgaggcc aacgtggatg ccacgcgccg
     1381 ctttgtcctg gacaatgact ttgtcacctt tggctggtac agctgccgcc gcgccatccc
     1441 ccgcctccag caccgggact cgtacgccga gctcgagtac gactgtgagg tgggcgacct
     1501 ctcggtccgg cgtgaagaca gctcctggcc ctcctaccag gccctggcct tcgatatcga
     1561 gtgtctgggg gaggagggct tccccacggc caccaacgag gctgacctga tcctgcagat
     1621 atcctgcgtc ctctggtcga caggggagga ggccgggcgc tataggcgca tcctgctgac
     1681 gctgggcacc tgcgaagaca tagagggggt tgaggtctac gagttcccat cggagctgga
     1741 catgctctac gccttcttcc agctcatcag agacctcagc gtggagattg tgaccggcta
     1801 caacgtggcc aactttgact ggccctacat tctggacaga gccaggcaca tctacagcat
     1861 caacccagcc tctctgggca aaattagggc tgggggcgtc tgcgaggtca ggcgacccca
     1921 tgatgcgggc aagggcttct tgcgggccaa caccaaggtc cgcatcaccg gcctcatccc
     1981 catcgacatg tacgccgtgt gccgggacaa gctcagcctc tcagactaca agctggacac
     2041 agtagccagg cacctactgg gggccaagaa ggaggatgtg cattacaagg agattcctcg
     2101 cctctttgca gcgggccccg aggggcgcag gcggctcggc atgtactgcg tgcaggactc
     2161 ggccctggtc atggatctgc taaaccattt cgtgatccac gtggaggtgg cagagattgc
     2221 caagatcgct cacatcccct gcaggcgggt gctggacgat gggcagcaga tccgcgtgtt
     2281 ctcctgcctc ctggcggccg cccaaaagga aaactttatc ctgcccatgc cctcggcctc
     2341 tgaccgggac ggctaccagg gggccaccgt catccagccc ctgtccggat tctacaactc
     2401 cccggttctg gtggtggact ttgccagcct ctacccgagc atcattcagg ctcataatct
     2461 ctgttattct accatgataa cgccgggaga agagcacagg ctagccggcc tgcgcccggg
     2521 agaagactat gagtccttca ggctcacggg gggcgtctac cactttgtaa agaagcacgt
     2581 gcacgagtcc ttcttggcta gtctgttgac ctcctggctg gccaagcgca aggccatcaa
     2641 gaagctgctg gcggcctgcg aggatccgcg ccaaaggacc atcctcgaca agcagcagct
     2701 ggccatcaag tgcacgtgca acgccgtcta cggcttcacc ggggtggcca acggcctctt
     2761 tccctgcctc tccatcgccg agacggtgac gctgcagggc cgcacgatgt tggagcgggc
     2821 caaggccttc gtggaggccc tgagccccgc caacctgcag gccctggccc cctccccgga
     2881 cgcctgggcg cccctcaacc ccgagggcca gcttcgagtc atctacgggg acacggactc
     2941 gctgtttatc gagtgccggg ggttttcaga gagcgagacc ctgcgctttg ccgatgccct
     3001 ggccgcccac accacccgga gcctgtttgt ggcccccatc tccctggagg ccgagaagac
     3061 cttctcctgc ctgatgctga ttacaaagaa gagatatgtg ggggtgctga cggacggcaa
     3121 gaccctgatg aagggggtgg agctcgtccg gaagacggcc tgcaagtttg tgcagacacg
     3181 ctgccggcgc gtgctcgacc tggtgctggc ggatgcccgg gtaaaggagg cggccagcct
     3241 cctctcccac cggcccttcc aagagtcatt tacacaaggg ctacctgtgg gctttttgcc
     3301 cgtcattgac atcctaaacc aggcctacac agacctccgt gaaggcaggg tccccatggg
     3361 ggagctctgc ttttcaacgg agctcagccg caagctctca gcctacaaga gcacccagat
     3421 gcctcacctg gccgtctacc agaagttcgt cgagcgcaac gaggaactgc cccagatcca
     3481 cgaccgcatc cagtacgtct ttgtggagcc caagggggga gtgaaggggg cgagaaagac
     3541 ggagatggcc gaggacccgg cctacgccga gcggcacggc gttcccgtgg ccgtggatca
     3601 ttatttcgac aagctgctcc aaggagcggc caacatcctc cagtgcctct ttgataacaa
     3661 ctccggggcc gccctctccg tcctccagaa ttttacagcc cggccaccat tcccaacttt
     3721 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     3781 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     3841 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     3901 ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa
     3961 tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     4021 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     4081 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     4141 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     4201 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     4261 gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga
     4321 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     4381 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     4441 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     4501 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     4561 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     4621 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     4681 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     4741 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     4801 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     4861 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     4921 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     4981 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     5041 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca
     5101 cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     5161 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5221 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5281 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     5341 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5401 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5461 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5521 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5581 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5641 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5701 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5761 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5821 tgctcacatg tt
//
