LOCUS       sequence-391897-        9409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human GLUT1 with K-->R mutations for all 11 cytosolic lysines and a 
            C-terminal GFP tag in pQCXIP vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Qualls-Histed SJ, Nielsen CP, MacGurn JA
  TITLE     Lysosomal trafficking of the glucose transporter GLUT1 requires 
            sequential regulation by TXNIP and ubiquitin.
  JOURNAL   iScience. 2023 Feb 6;26(3):106150. doi: 10.1016/j.isci.2023.106150. 
            eCollection 2023 Mar 17.
  PUBMED    36890792
REFERENCE   2  (bases 1 to 9409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2023-02-6"; volume: "26(3):106150. doi"; pages: " 
            10.1016/j.isci.2023.106150. eCollection 2023 Mar 17"
COMMENT     Sequence Label: 11K-cyto-->R GLUT1-GFP
FEATURES             Location/Qualifiers
     source          1..9409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(3742..3766)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3777..3779,3780..3782,3783..4496)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3810..3838)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4433..4453
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    join(4536..5110,5111..5113,5114..5122)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4703..4720)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4930..4949
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5142..5741
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5142..5161)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5638..5658
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6115..6540
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6643..6665)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6801..6821)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6806..7135
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6986..7121
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7157..7176
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7257..7274)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7428..8016)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7508..7527)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8187..8978,8979..9047))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8810..8829
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9048..9152)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9220..9238
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9338..9409,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca tggagcccag cagccggcgg ctgacgggtc
     2281 gcctcatgct ggccgtggga ggagcagtgc ttggctccct gcagtttggc tacaacactg
     2341 gagtcatcaa tgccccccag aaggtgatcg aggagttcta caaccagaca tgggtccacc
     2401 gctatgggga gagcatcctg cccaccacgc tcaccacgct ctggtccctc tcagtggcca
     2461 tcttttctgt tgggggcatg attggctcct tctctgtggg ccttttcgtt aaccgctttg
     2521 gccggcggaa ttcaatgctg atgatgaacc tgctggcctt cgtgtccgcc gtgctcatgg
     2581 gcttctcgaa actgggcaag tcctttgaga tgctgatcct gggccgcttc atcatcggtg
     2641 tgtactgcgg cctgaccaca ggcttcgtgc ccatgtatgt gggtgaagtg tcacccacag
     2701 cccttcgtgg ggccctgggc accctgcacc agctgggcat cgtcgtcggc atcctcatcg
     2761 cccaggtgtt cggcctggac tccatcatgg gcaacaagga cctgtggccc ctgctgctga
     2821 gcatcatctt catcccggcc ctgctgcagt gcatcgtgct gcccttctgc cccgagagtc
     2881 cccgcttcct gctcatcaac cgcaacgagg agaaccgggc ccggagtgtg ctacggcggc
     2941 tgcgcgggac agctgacgtg acccatgacc tgcaggagat gcgggaagag agtcggcaga
     3001 tgatgcggga gcggcgggtc accatcctgg agctgttccg ctcccccgcc taccgccagc
     3061 ccatcctcat cgctgtggtg ctgcagctgt cccagcagct gtctggcatc aacgctgtct
     3121 tctattactc cacgagcatc ttcgagaagg cgggggtgca gcagcctgtg tatgccacca
     3181 ttggctccgg tatcgtcaac acggccttca ctgtcgtgtc gctgtttgtg gtggagcgag
     3241 caggccggcg gaccctgcac ctcataggcc tcgctggcat ggcgggttgt gccatactca
     3301 tgaccatcgc gctagcactg ctggagcagc taccctggat gtcctatctg agcatcgtgg
     3361 ccatctttgg ctttgtggcc ttctttgaag tgggtcctgg ccccatccca tggttcatcg
     3421 tggctgaact cttcagccag ggtccacgtc cagctgccat tgccgttgca ggcttctcca
     3481 actggacctc aaatttcatt gtgggcatgt gcttccagta tgtggagcaa ctgtgtggtc
     3541 cctacgtctt catcatcttc actgtgctcc tggttctgtt cttcatcttc acctacttcc
     3601 gggttcctga gactcggggc cggaccttcg atgagatcgc ttccggcttc cggcaggggg
     3661 gagccagcca aagtgaccgg acacccgagg agctgttcca tcccctgggg gctgattccc
     3721 aagtgaaggg tgggcgcgcc gacccagctt tcttgtacaa agtggttcga tctagaatgg
     3781 ctagcaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     3841 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgct acatacggaa
     3901 agcttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
     3961 tcactacttt ctcttatggt gttcaatgct tttcccgtta tccggatcat atgaaacggc
     4021 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaacgcact atatctttca
     4081 aagatgacgg gaactacaag acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta
     4141 atcgtatcga gttaaaaggt attgatttta aagaagatgg aaacattctc ggacacaaac
     4201 tcgagtacaa ctataactca cacaatgtat acatcacggc agacaaacaa aagaatggaa
     4261 tcaaagctaa cttcaaaatt cgccacaaca ttgaagatgg atccgttcaa ctagcagacc
     4321 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     4381 tgtcgacaca atctgccctt tcgaaagatc ccaacgaaaa gcgtgaccac atggtccttc
     4441 ttgagtttgt aactgctgct gggattacac atggcatgga tgagctctac aaataattaa
     4501 ttaacggatc cggaattccg cccccccccc ccccccccct ctccctcccc cccccctaac
     4561 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     4621 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     4681 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     4741 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     4801 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     4861 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     4921 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     4981 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     5041 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     5101 cgatgataag cttgccacaa cccacaagga gacgaccttc catgaccgag tacaagccca
     5161 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
     5221 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
     5281 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     5341 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     5401 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     5461 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     5521 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     5581 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     5641 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     5701 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
     5761 agcgcccgac cgaaaggagc gcacgacccc atggctccga ccgaagccac ccggggcggc
     5821 cccgccgacc ccgcacccgc ccccgaggcc caccgactct agtcgagggc tgcagcgctg
     5881 cagaggccga gtgcagaact gctccaaagg gacctcaagg ctttccgagg gacactaggc
     5941 tgactccatc gagccagtgt agagataagc ttatcgatta gtccaatttg ttaaagacag
     6001 gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga
     6061 gtacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
     6121 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
     6181 aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagggtc
     6241 gaccctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
     6301 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
     6361 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
     6421 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
     6481 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt
     6541 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag
     6601 gtaagctggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     6661 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     6721 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     6781 cggagtgtag atccggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     6841 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     6901 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6961 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7021 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7081 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7141 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     7201 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     7261 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7321 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7381 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7441 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7501 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7561 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7621 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7681 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7741 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7801 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7861 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7921 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7981 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8041 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8101 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8161 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8221 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8281 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8341 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8401 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8461 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     8521 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8581 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8641 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8701 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8761 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     8821 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8881 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8941 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9001 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9061 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9121 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9181 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     9241 tttcgtcttc aagaattagc ttggccattg catacgttgt atccatatca taatatgtac
     9301 atttatattg gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat
     9361 taatagtaat caattacggg gtcattagtt catagcccat atatggagt
//
