LOCUS       sequence-391900-        5340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the ALN, PLN neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP052
FEATURES             Location/Qualifiers
     source          1..5340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2398..2547
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        3085..3189
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3190..3258,3259..4050)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3408..3427)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4221..4809
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4710..4729
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4963..4980
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5097..5118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5133..5138,5139..5156,5157..5163)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5171..5187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5176..5198
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5195..5211
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5195..5211
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgttgaatt accatccaga tgatcttcag ttagattgaa atttgatcgt
       61 atcacatctt caacagttga cggtgactga ataacaggaa cacattttgt catgctactt
      121 tttggaagaa ttgtacaatt ggcagaccca aagcatgtga tagttttgca gccagattgg
      181 gacggtgtaa ctggaaacag aaaaaaaaag attttcttga aaaaatgaga atattgtgaa
      241 aatgtatgtt tttataacat ttattttgaa aacaaaaata aaattttaaa aaaatctaat
      301 aattacccac ttttattctg atttgtgctc tgagttccaa ttgtttccaa aactgtaact
      361 tgaaataaaa gaagtagaaa cacaacagta gttttgtgct tcatcttccg aattttggac
      421 tgttcgagtg aaaattttga agaaaatcgt ctagatatca gaatgtttgt caaattatag
      481 ggtacaacac ataaaatatt tttatgtaat tttggaatat gcatctacga ttattccact
      541 ttttgagaaa tcgaaatgac tcacaatcat atactaggcc aacaggaaac atcagattta
      601 acaaaaaatt aattaaattt gaagtacagt gagggaaatg gagggaaaaa aaaatttaaa
      661 caatttttgg tcaaattttg aaaatatcac ttaacattat tttggaagtc gaccaaaata
      721 tgttttccta cgacttttaa attatgttca tgtgttgata ttaaaatcaa aatattttta
      781 atctgaatat tataggcgtt atgtttgtga caatgcttca aagtttctta aatctgttac
      841 agtacaccta cagtactcta ctagtactac taccgtacca ccatagtaaa cccacaatat
      901 tttcaaataa tttacgttga tattccatta agccagtttc tcgaattttc aattgcaagt
      961 aaaccgattt ttgacttttg tgtaaaattg tgtgcgtctt tgatctgaga agcttggaaa
     1021 tccggatttc agataatttt caccaactct agatcgcgta ttttccctat cagaccccct
     1081 agttctactt tgacagttca tatttcggtt gactttagtt tgatcaaata atgtcagcta
     1141 acaaaatacg ttcaaactat gtgtctataa tttggttgtt aggcattttt tgataaaagt
     1201 tacgacaacc ggtataagct gtcaaagtag atcatggtta tagaaaatga tgatagtatt
     1261 aataatcact atcagataca atcatcaaca gttagttaca tgtaaacttt tttcaaaaat
     1321 aatttattca ataatgatgt catcaaacta attttagtca tcatcagaaa gtcgggaaag
     1381 ttctcgactt tccttagcca cgcccacaat aaaacaaaga aaagtccaag ataggatagt
     1441 cccggattct gacgttttca tccatttccg gtgtctctaa acttcttttt tatctttttc
     1501 tcaaggaact tcgtcaacaa ctgccggcgg gtttcctgta gaagatgcta aatgaggaaa
     1561 agtttgaatc agaaatggcc gaatcgagcc acgctttcat gctgcacgac acatttcctg
     1621 taaaggattc ccacttcgtc atactaaaat atagactata taaatgattt gtccaaacga
     1681 tgtgaagcat tattttgtat aactagaact ctacattcga ttattccatt ttttgtattt
     1741 tttcaagttt taggtacccc aaaaaggcgc gcctctagag gatccccggg attggccaaa
     1801 ggacccaaag gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc
     1861 ttggctagga tgggccctaa aaagaagcgt aaagctagca tgaagctgtt gtcttcaatg
     1921 gaacaagctt gcgacatatg ccggttaaaa aaactcaggt gctcgaagga aaagcccaag
     1981 tgtgccaagt gtctgaagaa caactcggag tgccgttatt ctcccaaggc tatgaggtcc
     2041 ccactgacca gggcgcatct gacagaggta gaatcaaggt tggaaaaatt ggaagatttg
     2101 tttctgctca tgtttcctcg ggaaaacctg gacagcattt tgaacatgga ctctttggac
     2161 gaagtgaaag taatgttgaa acaattatat atgcaggact atggtgacac ggatgctgct
     2221 atggatagct tgacccctat ggaaaatggc atgtctgaag ggccgagaaa acacatgaag
     2281 actgcgacat catcgccaaa agtggatgaa gatggaagcc aaagtcagtt gagtgtatcg
     2341 ccggaattcc cgctgcagtc tggtggcgga ggggctccta ggggttccgg acgggctgac
     2401 gcattggacg attttgatct ggatatgctg ggaagtgacg ccctcgatga ttttgacctt
     2461 gacatgcttg gttcggatgc ccttgatgac tttgacctcg acatgctcgg cagtgacgcc
     2521 cttgatgatt tcgacctgga catgctgatt aactaaggta ccgaattcgc tagccggcca
     2581 tacaagtaat ccggatgatc gacgccaacg tcgttgaatt ttcaaatttt aaatactgaa
     2641 tatttgtttt ttttcctatt atttatttat tctctttgtg ttttttttct tgctttctaa
     2701 aaaattaatt caatccaaat ctaaacattt ttttttctct ttccgtctcc caattcgtat
     2761 tccgctcctc tcatctgaac acaatgtgca agtttattta tcttctcgct ttcatttcat
     2821 taggacgtgg ggggaattgg tggaaggggg aaacacacaa aaggatgatg gaaatgaaat
     2881 aaggacacac aatatgcaac aacattcaat tcagaaatat ggaggaaggt ttaaaagaaa
     2941 acataaaaat atatagagga ggaaggaaaa ctagtaaaaa ataagcaaag aaattaggcg
     3001 aacgatgaga attgtcctcg cttgggccca aaaggccttt tttttttggc gccgacgtca
     3061 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3121 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     3181 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     3241 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     3301 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     3361 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     3421 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     3481 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     3541 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     3601 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     3661 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     3721 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     3781 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     3841 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     3901 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     3961 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     4021 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     4081 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     4141 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4201 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4261 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4321 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     4381 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4441 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4501 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4561 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4621 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4681 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4741 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4801 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4861 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     4921 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     4981 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     5041 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     5101 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     5161 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     5221 gccaagctgt aagtttaaac atgatcttac taactaacta ttctcattta aattttcaga
     5281 gcttaaaaat ggctgaaatc actcacaacg atggatacgc taacaacttg gaaatgaaat
//
