LOCUS       sequence-391902-        5194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the IL2 neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pMZ3
FEATURES             Location/Qualifiers
     source          1..5194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2252..2401
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        2939..3043
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3044..3112,3113..3904)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3262..3281)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4075..4663
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4564..4583
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4817..4834
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4951..4972
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4987..4992,4993..5010,5011..5017)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5025..5041
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5030..5052
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5049..5065
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5049..5065
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccattg tttttttatt gagccttttt
       61 gatgggcatt gtctagtcat tgaaaatatt aacagaaatt ataaaattca ccaaaaaatt
      121 cattaaagca tttttccata tgctttaaaa ttttcaagca taccaaagtt ttcctgtttt
      181 cttgtttcgt tatgggtttt ttagttttca tttagcttac acccccccca acgtcccaga
      241 caatttcaac aaatcttggt tgtgattccg tttcaaaacc tcacctatac attttctata
      301 aataaagcaa attattttta aaaatacctt ttatatatat ttttcctttt ccaccctcca
      361 atttatcaca ttgaaaataa aaatcataaa acccccgttt ttcagagtcg acgacttcaa
      421 taatttttat ttgctttttt aaaattattt tttcgttttg tttttttggt cgaataaatc
      481 tattaaaatg ggaaatataa ttgtttttgt tcacataacg agaatttcag agatgtaggt
      541 tctggtcaat tccgtaaatc actgacactc caaagttatg aaatgccgca aagactactg
      601 taggcagtat caattgaatc aattgttaga aacctgtttc gagaagtttt ggaatattcg
      661 tatttcaaca ttttattggc tctaaattgt tcaaaaatct caaattttgg aaagtttggt
      721 aagttgccaa aatgactaga tacttttaaa tatctaacac gttttggagt ttgctacgat
      781 attcgggttt tttggataat tatattcata agtaaaaaat cggataaatg ctgtcaaagt
      841 tgaaataaac tatccgaggc tttatcaaaa aatcaattgt tttcagaaat tctgtgcatt
      901 tattcaactt tttgttctct gattccaatc ttcaatcttc attaaataca acttcataca
      961 aatttttctt cttcacatca aacaccgttt ggtccgtttc ctttcgtcgc ttggagacct
     1021 acatggcaac attgactcca cgtgtatgta gaagaagggt cccattctcc acgctttact
     1081 acactctata ctgaattttc catttatttt cttgtccttt tcattttaaa aacatttttc
     1141 cgttcggatt tgtttacaaa ttttgttctc tctgatagaa gtattcaatt attatacttt
     1201 taatctgtaa attaaaaaaa aaaactaaat ccttttctca tatcacggga acaataaatc
     1261 atgagaatgc gtacttggtg cattttattt gacgcgcgat atctcttatc gaaaactaca
     1321 gtaagagctt aaatatctat tgttatttga gttcttactg tagttttcgc taagagatat
     1381 cgcgcgtcaa ataaaatgca ccaagtacac attctcatga tttattgttc ccgtaatacc
     1441 aaccaattat tgcgacgcaa taccttcata tacatttttt aattttaaaa atttttttta
     1501 aaatatagtt gagatccttg ttgtagtacg gtagtagtac gaagatattc caatgtttct
     1561 ttaaaaaaat tttccaacca aatttagttc taaatttatt agttgaactt ttcagaatag
     1621 gcgcgcctct agaggatccc cgggattggc caaaggaccc aaaggtatgt ttcgaatgat
     1681 actaacataa catagaacat tttcaggagg acccttggct aggatgggcc ctaaaaagaa
     1741 gcgtaaagct agcatgaagc tgttgtcttc aatggaacaa gcttgcgaca tatgccggtt
     1801 aaaaaaactc aggtgctcga aggaaaagcc caagtgtgcc aagtgtctga agaacaactc
     1861 ggagtgccgt tattctccca aggctatgag gtccccactg accagggcgc atctgacaga
     1921 ggtagaatca aggttggaaa aattggaaga tttgtttctg ctcatgtttc ctcgggaaaa
     1981 cctggacagc attttgaaca tggactcttt ggacgaagtg aaagtaatgt tgaaacaatt
     2041 atatatgcag gactatggtg acacggatgc tgctatggat agcttgaccc ctatggaaaa
     2101 tggcatgtct gaagggccga gaaaacacat gaagactgcg acatcatcgc caaaagtgga
     2161 tgaagatgga agccaaagtc agttgagtgt atcgccggaa ttcccgctgc agtctggtgg
     2221 cggaggggct cctaggggtt ccggacgggc tgacgcattg gacgattttg atctggatat
     2281 gctgggaagt gacgccctcg atgattttga ccttgacatg cttggttcgg atgcccttga
     2341 tgactttgac ctcgacatgc tcggcagtga cgcccttgat gatttcgacc tggacatgct
     2401 gattaactaa ggtaccgaat tcgctagccg gccatacaag taatccggat gatcgacgcc
     2461 aacgtcgttg aattttcaaa ttttaaatac tgaatatttg ttttttttcc tattatttat
     2521 ttattctctt tgtgtttttt ttcttgcttt ctaaaaaatt aattcaatcc aaatctaaac
     2581 attttttttt ctctttccgt ctcccaattc gtattccgct cctctcatct gaacacaatg
     2641 tgcaagttta tttatcttct cgctttcatt tcattaggac gtggggggaa ttggtggaag
     2701 ggggaaacac acaaaaggat gatggaaatg aaataaggac acacaatatg caacaacatt
     2761 caattcagaa atatggagga aggtttaaaa gaaaacataa aaatatatag aggaggaagg
     2821 aaaactagta aaaaataagc aaagaaatta ggcgaacgat gagaattgtc ctcgcttggg
     2881 cccaaaaggc cttttttttt tggcgccgac gtcaggtggc acttttcggg gaaatgtgcg
     2941 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3001 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3061 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3121 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3181 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3241 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     3301 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3361 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3421 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3481 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3541 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3601 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     3661 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3721 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3781 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3841 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3901 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     3961 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4021 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4081 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4141 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4201 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4261 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4321 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4381 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4441 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4501 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4561 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4621 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4681 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4741 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     4801 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     4861 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     4921 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     4981 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     5041 atttcacaca ggaaacagct atgaccatga ttacgccaag ctgtaagttt aaacatgatc
     5101 ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga aatcactcac
     5161 aacgatggat acgctaacaa cttggaaatg aaat
//
