LOCUS       sequence-391905-        9385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human GLUT1 with a single cytosolic lysine (K245) and C-terminal GFP
            tag in pQCXIP vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9385)
  AUTHORS   Qualls-Histed SJ, Nielsen CP, MacGurn JA
  TITLE     Lysosomal trafficking of the glucose transporter GLUT1 requires 
            sequential regulation by TXNIP and ubiquitin.
  JOURNAL   iScience. 2023 Feb 6;26(3):106150. doi: 10.1016/j.isci.2023.106150. 
            eCollection 2023 Mar 17.
  PUBMED    36890792
REFERENCE   2  (bases 1 to 9385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2023-02-6"; volume: "26(3):106150. doi"; pages: " 
            10.1016/j.isci.2023.106150. eCollection 2023 Mar 17"
COMMENT     Sequence Label: 1K245 GLUT1-GFP
FEATURES             Location/Qualifiers
     source          1..9385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(3742..3766)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3777..3779,3780..3782,3783..4496)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3810..3838)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4433..4453
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    4517..5085
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4679..4696)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4906..4925
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5118..5717
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5118..5137)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5614..5634
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6091..6516
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6619..6641)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6777..6797)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6782..7111
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6962..7097
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7133..7152
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7233..7250)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7404..7992)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7484..7503)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8163..8954,8955..9023))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8786..8805
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9024..9128)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9196..9214
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9314..9385,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca tggagcccag cagccggcgg ctgacgggtc
     2281 gcctcatgct ggccgtggga ggagcagtgc ttggctccct gcagtttggc tacaacactg
     2341 gagtcatcaa tgccccccag aaggtgatcg aggagttcta caaccagaca tgggtccacc
     2401 gctatgggga gagcatcctg cccaccacgc tcaccacgct ctggtccctc tcagtggcca
     2461 tcttttctgt tgggggcatg attggctcct tctctgtggg ccttttcgtt aaccgctttg
     2521 gccggcggaa ttcaatgctg atgatgaacc tgctggcctt cgtgtccgcc gtgctcatgg
     2581 gcttctcgaa actgggcaag tcctttgaga tgctgatcct gggccgcttc atcatcggtg
     2641 tgtactgcgg cctgaccaca ggcttcgtgc ccatgtatgt gggtgaagtg tcacccacag
     2701 cccttcgtgg ggccctgggc accctgcacc agctgggcat cgtcgtcggc atcctcatcg
     2761 cccaggtgtt cggcctggac tccatcatgg gcaacaagga cctgtggccc ctgctgctga
     2821 gcatcatctt catcccggcc ctgctgcagt gcatcgtgct gcccttctgc cccgagagtc
     2881 cccgcttcct gctcatcaac cgcaacgagg agaaccgggc ccggagtgtg ctacggcggc
     2941 tgcgcgggac agctgacgtg acccatgacc tgcaggagat gaaggaagag agtcggcaga
     3001 tgatgcggga gcggcgggtc accatcctgg agctgttccg ctcccccgcc taccgccagc
     3061 ccatcctcat cgctgtggtg ctgcagctgt cccagcagct gtctggcatc aacgctgtct
     3121 tctattactc cacgagcatc ttcgagaagg cgggggtgca gcagcctgtg tatgccacca
     3181 ttggctccgg tatcgtcaac acggccttca ctgtcgtgtc gctgtttgtg gtggagcgag
     3241 caggccggcg gaccctgcac ctcataggcc tcgctggcat ggcgggttgt gccatactca
     3301 tgaccatcgc gctagcactg ctggagcagc taccctggat gtcctatctg agcatcgtgg
     3361 ccatctttgg ctttgtggcc ttctttgaag tgggtcctgg ccccatccca tggttcatcg
     3421 tggctgaact cttcagccag ggtccacgtc cagctgccat tgccgttgca ggcttctcca
     3481 actggacctc aaatttcatt gtgggcatgt gcttccagta tgtggagcaa ctgtgtggtc
     3541 cctacgtctt catcatcttc actgtgctcc tggttctgtt cttcatcttc acctacttcc
     3601 gggttcctga gactcggggc cggaccttcg atgagatcgc ttccggcttc cggcaggggg
     3661 gagccagcca aagtgaccgg acacccgagg agctgttcca tcccctgggg gctgattccc
     3721 aagtgaaggg tgggcgcgcc gacccagctt tcttgtacaa agtggttcga tctagaatgg
     3781 ctagcaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     3841 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgct acatacggaa
     3901 agcttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
     3961 tcactacttt ctcttatggt gttcaatgct tttcccgtta tccggatcat atgaaacggc
     4021 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaacgcact atatctttca
     4081 aagatgacgg gaactacaag acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta
     4141 atcgtatcga gttaaaaggt attgatttta aagaagatgg aaacattctc ggacacaaac
     4201 tcgagtacaa ctataactca cacaatgtat acatcacggc agacaaacaa aagaatggaa
     4261 tcaaagctaa cttcaaaatt cgccacaaca ttgaagatgg atccgttcaa ctagcagacc
     4321 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     4381 tgtcgacaca atctgccctt tcgaaagatc ccaacgaaaa gcgtgaccac atggtccttc
     4441 ttgagtttgt aactgctgct gggattacac atggcatgga tgagctctac aaataattaa
     4501 ttaacggatc cggaattccg cycccccccc cctaacgtta ctggccgaag ccgcttggaa
     4561 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     4621 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     4681 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     4741 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     4801 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     4861 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     4921 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     4981 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     5041 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaaccca
     5101 caaggagacg accttccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     5161 acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     5221 acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     5281 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     5341 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     5401 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     5461 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     5521 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     5581 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     5641 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     5701 gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
     5761 gaccccatgg ctccgaccga agccacccgg ggcggccccg ccgaccccgc acccgccccc
     5821 gaggcccacc gactctagtc gagggctgca gcgctgcaga ggccgagtgc agaactgctc
     5881 caaagggacc tcaaggcttt ccgagggaca ctaggctgac tccatcgagc cagtgtagag
     5941 ataagcttat cgattagtcc aatttgttaa agacaggata tcagtggtcc aggctctagt
     6001 tttgactcaa caatatcacc agctgaagcc tatagagtac gagccataga taaaataaaa
     6061 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     6121 gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa
     6181 gttcagatca aggtcaggaa cagatggaac agggtcgacc ctagagaacc atcagatgtt
     6241 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
     6301 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
     6361 cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat
     6421 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
     6481 gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag
     6541 acccctgccc agggaccacc gacccaccac cgggaggtaa gctggctgcc tcgcgcgttt
     6601 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     6661 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     6721 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtagatcc ggctgtggaa
     6781 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6841 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     6901 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     6961 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7021 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7081 aggctttttt ggaggcctag gcttttgcaa aaagcttact ggcttaacta tgcggcatca
     7141 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     7201 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7261 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7321 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7381 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7441 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7501 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7561 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7621 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7681 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7741 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7801 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     7861 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7921 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7981 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8041 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8101 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8161 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8221 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8281 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8341 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8401 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8461 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8521 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8581 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8641 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8701 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8761 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     8821 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8881 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8941 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9001 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9061 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9121 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     9181 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attagcttgg
     9241 ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca
     9301 ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     9361 ttagttcata gcccatatat ggagt
//
