LOCUS       sequence-391911-        8764 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modular CD8hinge-CD8transmembrane-41BB-CD3z CAR backbone, For 
            Transient Expression or Lentiviral Production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Timpanaro A, Piccand C, Dzhumashev D, Anton-Joseph S, Robbi A, Moser
            J, Rossler J, Bernasconi M
  TITLE     CD276-CAR T cells and Dual-CAR T cells targeting CD276/FGFR4 promote
            rhabdomyosarcoma clearance in orthotopic mouse models.
  JOURNAL   J Exp Clin Cancer Res. 2023 Nov 4;42(1):293. doi: 
            10.1186/s13046-023-02838-3.
  PUBMED    37924157
REFERENCE   2  (bases 1 to 8764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Clin 
            Cancer Res."; date: "2023-11-4"; volume: "42(1):293. doi"; pages: " 
            10.1186/s13046-023-02838-3"
COMMENT     Sequence Label: pLV-EF1A-CD19.8H.8TM.BB.3z
FEATURES             Location/Qualifiers
     source          1..8764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1965..2175
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     protein_bind    2181..2205
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2272..2301
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2302..2331
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3784..3840
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3841..3843,3844..4557)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3841..3843,3844..4554)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3883..3904)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4144..4163)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4491..4512
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4567..4591)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4609..5197
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4662..4682)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5080..5091)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5285..5518
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5590..5711
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5627..5646)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5681..5700
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5751..5886
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5906..5925)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5907..5925)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5935..5952)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5935..5951)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5944..5966)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6093..6548
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6180..6199)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6390..6411
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6574..6678
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6679..6747,6748..7539)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6897..6916)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7710..8298
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8199..8218
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8452..8469
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8586..8607
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8622..8627,8628..8645,8646..8652)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8660..8676
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8665..8687
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8684..8700
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8684..8700
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8719..8739
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8721..8739
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgtc tagagggcag agcgcacatc
     1981 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     2041 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     2101 tggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     2161 gccgccagaa cacagggatc ccaagtttgt acaaaaaagc aggctgccac catgctcagg
     2221 ctgctcttgg ctctcaactt attcccttca attcaagtaa caggactcga ggaacaaaaa
     2281 ctcatctcag aagaggatct ggaacaaaag ctcatttcag aagaagatct gctcgaggtc
     2341 gacggagacg ccgacatcca gatgacacag actacatcct ccctgtctgc ctctctggga
     2401 gacagagtca ccatcagttg cagggcaagt caggacatct ctaagtattt gaattggtat
     2461 cagcagaaac cagatggaac tgttaaactc ctgatctacc atacatcaag attacactca
     2521 ggagtcccat caaggttcag tggcagtggg tctggaacag attattctct caccattagc
     2581 aacctggagc aagaagatat tgccacttac ttttgccaac agggtaatac gcttccgtac
     2641 acgttcggag gggggactaa gttggaaata acaggctcca cctctggatc cggcaagccc
     2701 ggatctggcg agggatccac caagggcgag gtgaaactgc aggagtcagg acctggcctg
     2761 gtggcgccct cacagagcct gtccgtcaca tgcactgtct caggggtctc attacccgac
     2821 tatggtgtaa gctggattcg ccagcctcca cgaaagggtc tggagtggct gggagtaata
     2881 tggggtagtg aaaccacata ctataattca gctctcaaat ccagactgac catcatcaag
     2941 gacaactcca agagccaagt tttcttaaaa atgaacagtc tgcaaactga tgacacagcc
     3001 atttactact gtgccaaaca ttattactac ggtggtagct atgctatgga ctactggggt
     3061 caaggaacct cagtcaccgt ctccggtacc accacgacgc cagcgccgcg accaccaaca
     3121 ccggcgccca ccatcgcgtc gcagcccctg tccctgcgcc cagaggcgtg ccggccagcg
     3181 gcgggtggcg cagtgcacac gagggggctg gacttcgcct gtgatatcta catctgggcg
     3241 cccttggccg ggacttgtgg ggtccttctc ctgtcactgg ttatcaccct ttactgcaaa
     3301 cggggcagaa agaaactcct gtatatattc aaacaaccat ttatgagacc agtacaaact
     3361 actcaagagg aagatggctg tagctgccga tttccagaag aagaagaagg aggatgtgaa
     3421 ctgtccggac tgagagtgaa gttcagcagg agcgcagacg cccccgcgta ccagcagggc
     3481 cagaaccagc tctataacga gctcaatcta ggacgaagag aggagtacga tgttttggac
     3541 aagaggcgtg gccgggaccc tgagatgggg ggaaagccgc agagaaggaa gaaccctcag
     3601 gaaggcctgt acaatgaact gcagaaagat aagatggcgg aggcctacag tgagattggg
     3661 atgaaaggcg agcgccggag gggcaagggg cacgatggcc tttaccaggg tctcagtaca
     3721 gccaccaagg acacctacga cgcccttcac atgcaggccc tgccccctcg ctccggacgt
     3781 acggccacga acttctctct gttaaagcaa gcaggcgacg tggaagaaaa ccccggtccc
     3841 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     3901 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     3961 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     4021 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     4081 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     4141 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     4201 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     4261 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     4321 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     4381 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     4441 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     4501 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaggg
     4561 ccccgtaccc agctttcttg tacaaagtgg tgataatcga attccgataa tcaacctctg
     4621 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     4681 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     4741 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     4801 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     4861 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     4921 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4981 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     5041 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5101 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     5161 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gggaattccc gcggttcgct
     5221 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5281 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     5341 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5401 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5461 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     5521 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     5581 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5641 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5701 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     5761 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5821 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5881 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     5941 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6001 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6061 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     6121 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6181 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6241 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6301 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6361 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6421 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6481 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     6541 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6601 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6661 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6721 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6781 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6841 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6901 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6961 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     7021 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7081 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7141 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7201 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7261 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7321 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7381 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7441 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7501 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7561 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7621 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7681 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7741 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7801 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7861 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7921 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7981 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8041 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8101 ctatgagaaa gcgccacgct tcccgaagag agaaaggcgg acaggtatcc ggtaagcggc
     8161 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8221 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8281 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8341 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8401 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8461 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8521 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     8581 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8641 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     8701 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     8761 gctt
//
