LOCUS       sequence-391922-        9305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of stdMCP-PH in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9305)
  AUTHORS   Liang Y, Xu H, Cheng T, Fu Y, Huang H, Qian W, Wang J, Zhou Y, Qian 
            P, Yin Y, Xu P, Zou W, Chen B
  TITLE     Gene activation guided by nascent RNA-bound transcription factors.
  JOURNAL   Nat Commun. 2022 Nov 28;13(1):7329. doi: 10.1038/s41467-022-35041-7.
  PUBMED    36443367
REFERENCE   2  (bases 1 to 9305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-11-28"; volume: "13(1):7329. doi"; pages: " 
            10.1038/s41467-022-35041-7"
COMMENT     Sequence Label: pCA24-pHR-CMV-stdMCP-p65-HSF1-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..9305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(110..129)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        301..680
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        682..880
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             898..1078
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1125..1250
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1747..1980
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2165..2209
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2507..2624
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3163..3466
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3467..3670
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4424..4966
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             4991..5362
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             5378..5431
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5432..5434,5435..6148)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5432..5434,5435..6145)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5474..5495)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5735..5754)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6082..6103
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6168..6201)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6257..6845
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6310..6330)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6728..6739)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6848..6864)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6849..6865)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7374..7554
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7616..8204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7696..7715)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8375..9166,9167..9235))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8998..9017
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9236..9305,1..35))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
       61 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
      121 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
      181 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
      241 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
      301 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      361 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      421 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      481 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      541 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      601 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      661 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      721 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      781 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      841 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      901 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      961 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1021 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1081 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1141 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1201 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1261 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1321 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1381 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1441 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1501 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1561 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
     1621 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     1681 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     1741 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     1801 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     1861 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     1921 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     1981 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     2041 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     2101 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     2161 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     2221 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     2281 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     2341 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     2401 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
     2461 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     2521 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2581 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2641 acagcagaga tccagtttgg ttagtaccgg gcccgctcta gacgctatgt ggatacgctg
     2701 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     2761 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     2821 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     2881 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     2941 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3001 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgctcga gtactaggat
     3061 ccattaggcg gccgcgtgga taaccgtatt accgccatgc attagttatt aatagtaatc
     3121 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     3181 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     3241 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     3301 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     3361 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     3421 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     3481 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     3541 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     3601 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     3661 aagcagagct ggtttagtga accgtcagat ccgctagccc accatggctt ctaactttac
     3721 tcagttcgtt ctcgtcgaca atggcggaac tggcgacgtg actgtcgccc caagcaactt
     3781 cgctaacggg atcgctgaat ggatcagctc taactcgcgt tcacaggctt acaaagtaac
     3841 ctgtagcgtt cgtcagagct ctgcgcagaa tcgcaaatac accatcaaag tcgaggtgcc
     3901 taaaggcgcc tggcgttcgt acttaaatat ggaactaacc attccaattt tcgccacgaa
     3961 ttccgactgc gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat
     4021 tccctcagca atcgcagcaa actccggcat ctacgccatg gccagcaact tcacccagtt
     4081 cgtgctggtg gacaacggcg gcaccggcga cgtgaccgtg gcccccagca acttcgccaa
     4141 cggcatcgcc gagtggatca gcagcaacag cagaagccag gcctacaagg tgacctgcag
     4201 cgtgagacag agcagcgccc agaacagaaa gtacaccatc aaggtggagg tgcccaaggg
     4261 cgcctggaga agctacctga acatggagct gaccatcccc atcttcgcca ccaacagcga
     4321 ctgcgagctg atcgtgaagg ccatgcaggg cctgctgaag gacggcaacc ccatccccag
     4381 cgccatcgcc gccaacagcg gcatctacgc cgacccaccg gtcccttcag ggcagatcag
     4441 caaccaggcc ctggctctgg cccctagctc cgctccagtg ctggcccaga ctatggtgcc
     4501 ctctagtgct atggtgcctc tggcccagcc acctgctcca gcccctgtgc tgaccccagg
     4561 accaccccag tcactgagcg ctccagtgcc caagtctaca caggccggcg aggggactct
     4621 gagtgaagct ctgctgcacc tgcagttcga cgctgatgag gacctgggag ctctgctggg
     4681 gaacagcacc gatcccggag tgttcacaga tctggcctcc gtggacaact ctgagtttca
     4741 gcagctgctg aatcagggcg tgtccatgtc tcatagtaca gccgaaccaa tgctgatgga
     4801 gtaccccgaa gccattaccc ggctggtgac cggcagccag cggccccccg accccgctcc
     4861 aactcccctg ggaaccagcg gcctgcctaa tgggctgtcc ggagatgaag acttctcaag
     4921 catcgctgat atggacttta gtgccctgct gtcacagatt tcctctagtg ggcagggagg
     4981 aggtggaagc ggcttcagcg tggacaccag tgccctgctg gacctgttca gcccctcggt
     5041 gaccgtgccc gacatgagcc tgcctgacct tgacagcagc ctggccagta tccaagagct
     5101 cctgtctccc caggagcccc ccaggcctcc cgaggcagag aacagcagcc cggattcagg
     5161 gaagcagctg gtgcactaca cagcgcagcc gctgttcctg ctggaccccg gctccgtgga
     5221 caccgggagc aacgacctgc cggtgctgtt tgagctggga gagggctcct acttctccga
     5281 aggggacggc ttcgccgagg accccaccat ctccctgctg acaggctcgg agcctcccaa
     5341 agccaaggac cccactgtct ccgctagcgg cagtggagag ggcagaggaa gtctgctaac
     5401 atgcggtgac gtcgaggaga atcctggccc agtgagcaag ggcgaggagc tgttcaccgg
     5461 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     5521 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     5581 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     5641 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     5701 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     5761 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     5821 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     5881 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     5941 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     6001 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     6061 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     6121 tctcggcatg gacgagctgt acaagtaaga attcgtcgag ggacctaata acttcgtata
     6181 gcatacatta tacgaagtta tacatgttta agggttccgg ttccactagg tacaattcga
     6241 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6301 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6361 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6421 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6481 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6541 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     6601 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     6661 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     6721 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     6781 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     6841 cccgcatcga taccgtcgac ctcgatcgag acctagaaaa acatggagca atcacaagta
     6901 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     6961 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     7021 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     7081 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     7141 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     7201 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     7261 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     7321 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     7381 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7441 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7501 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatgt
     7561 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7621 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7681 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7741 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7801 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7861 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7921 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7981 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8041 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8101 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8161 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8221 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8281 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8341 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8401 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8461 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8521 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8581 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8641 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8701 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8761 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8821 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8881 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8941 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9001 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9061 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9121 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9181 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9241 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9301 ttgaa
//
