LOCUS       sequence-391925-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac Vector expressing KEIMA-RAMP4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Hoyer MJ, Capitanio C, Smith IR, Paoli JC, Bieber A, Jiang Y, Paulo 
            JA, Gonzalez-Lozano MA, Baumeister W, Wilfling F, Schulman BA, 
            Harper JW
  TITLE     Combinatorial selective ER-phagy remodels the ER during 
            neurogenesis.
  JOURNAL   Nat Cell Biol. 2024 Mar;26(3):378-392. doi: 
            10.1038/s41556-024-01356-4. Epub 2024 Mar 1.
  PUBMED    38429475
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-024-01356-4"; journalName: "Nat Cell Biol"; date: 
            "2024-03"; volume: "26"; issue: "3"; pages: "378-392"
COMMENT     Sequence Label: pAC150-KEIMA-RAMP4
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..29
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(38..40,41..43,44..703)
                     /codon_start=1
                     /product="humanized monomeric Keima-Red fluorescent 
                     protein"
                     /label=hmKeima-Red
                     /note="mammalian codon-optimized"
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
     protein_bind    complement(933..957)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggggacaagt ttgtacaaaa aagcaggctg cggtaccatg gtgagcgtga tcgccaagca
       61 gatgacctac aaggtgtaca tgagcggcac cgtgaacggc cactacttcg aggtggaggg
      121 cgacggcaag ggcaagccct acgagggcga gcagaccgtg aagctgaccg tgaccaaggg
      181 tggccccctg cccttcgcct gggacatcct gagcccccag ctccagtacg gcagcatccc
      241 cttcaccaag taccccgagg acatccccga ctacttcaag cagagcttcc ccgagggcta
      301 cacctgggag cgcagcatga acttcgagga cggcgccgtg tgcaccgtga gcaacgacag
      361 cagcatccag ggcaactgct tcatctacaa cgtgaagatc agcggcgaga acttcccccc
      421 caacggcccc gtgatgcaga agaagaccca gggctgggag cccagcaccg agcgcctgtt
      481 cgcccgcgac ggaatgctga tcggcaacga ctacatggcc ctgaagctgg agggcggcgg
      541 ccactacctg tgcgagttca agagcaccta caaggccaag aagcccgtga ggatgcccgg
      601 ccgccacgag atcgaccgca agctggacgt gaccagccac aaccgcgact acaccagcgt
      661 ggagcagtgc gagatcgcca tcgcccgcca ctccctgctg ggcggaggct caggaggctc
      721 aggaggctca atggtcgcca agcaaaggat ccgtatggcc aacgagaagc acagcaagaa
      781 catcacccag cgcggcaacg tcgccaagac ctcgagaaat gcccccgaag agaaggcgtc
      841 tgtaggaccc tggttattgg ctctcttcat ttttgttgtc tgtggttctg caattttcca
      901 gattattcaa agtatcagga tgggcatgtg agacccagct ttcttgtaca aagtggtccc
      961 c
//
