LOCUS       sequence-391927-        9350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of stdPCP-PH in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9350)
  AUTHORS   Liang Y, Xu H, Cheng T, Fu Y, Huang H, Qian W, Wang J, Zhou Y, Qian 
            P, Yin Y, Xu P, Zou W, Chen B
  TITLE     Gene activation guided by nascent RNA-bound transcription factors.
  JOURNAL   Nat Commun. 2022 Nov 28;13(1):7329. doi: 10.1038/s41467-022-35041-7.
  PUBMED    36443367
REFERENCE   2  (bases 1 to 9350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-11-28"; volume: "13(1):7329. doi"; pages: " 
            10.1038/s41467-022-35041-7"
COMMENT     Sequence Label: pCA25-pHR-CMV-stdPCP-p65-HSF1-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..9350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(110..129)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        301..680
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        682..880
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             898..1078
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1125..1250
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1747..1980
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2165..2209
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2507..2624
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3163..3466
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3467..3670
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4469..5011
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             5036..5407
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             5423..5476
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5477..5479,5480..6193)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5477..5479,5480..6190)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5519..5540)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5780..5799)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6127..6148
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6213..6246)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6302..6890
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6355..6375)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6773..6784)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6893..6909)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6894..6910)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7419..7599
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7661..8249)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7741..7760)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8420..9211,9212..9280))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9043..9062
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9281..9350,1..35))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
       61 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
      121 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
      181 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
      241 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
      301 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      361 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      421 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      481 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      541 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      601 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      661 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      721 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      781 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      841 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      901 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      961 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1021 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1081 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1141 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1201 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1261 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1321 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1381 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1441 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1501 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1561 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
     1621 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     1681 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     1741 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     1801 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     1861 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     1921 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     1981 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     2041 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     2101 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     2161 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     2221 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     2281 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     2341 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     2401 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
     2461 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     2521 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2581 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2641 acagcagaga tccagtttgg ttagtaccgg gcccgctcta gacgctatgt ggatacgctg
     2701 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     2761 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     2821 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     2881 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     2941 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3001 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgctcga gtactaggat
     3061 ccattaggcg gccgcgtgga taaccgtatt accgccatgc attagttatt aatagtaatc
     3121 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     3181 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     3241 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     3301 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     3361 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     3421 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     3481 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     3541 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     3601 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     3661 aagcagagct ggtttagtga accgtcagat ccgctagccc accatgttgg cttccaaaac
     3721 tatcgttctg agcgtaggtg aagcgaccag aacgttaact gaaattcaat ctacagcaga
     3781 caggcaaata tttgaagaga aagtcggtcc attagtaggg aggttaagat tgacagctag
     3841 cttaagacaa aatggagcta aaactgcata cagggtcaat ttaaaattgg atcaagcaga
     3901 tgtcgttgat agcggtctac ctaaggttag atacacgcaa gtgtggtcac acgatgtcac
     3961 tattgtcgcg aattctaccg aagctagtag aaaatcattg tatgatttaa caaaatcctt
     4021 ggtggctacc tctcaggtgg aagatcttgt tgtgaattta gttccattag gtagggcgga
     4081 tccgctagcc tccaaaacca tcgttctttc ggtcggcgag gctactcgca ctctgactga
     4141 gatccagtcc accgcagacc gtcagatctt cgaagagaag gtcgggcctc tggtgggtcg
     4201 gctgcgcctc acggcttcgc tccgtcaaaa cggagccaag accgcgtatc gcgtcaacct
     4261 aaaactggat caggcggacg tcgttgattc cggacttccg aaagtgcgct acactcaggt
     4321 atggtcgcac gacgtgacaa tcgttgcgaa tagcaccgag gcctcgcgca aatcgttgta
     4381 cgatttgacc aagtccctcg tcgcgacctc gcaggtcgaa gatcttgtcg tcaaccttgt
     4441 gccgctgggc cgtccaccgg tcgccacccc ttcagggcag atcagcaacc aggccctggc
     4501 tctggcccct agctccgctc cagtgctggc ccagactatg gtgccctcta gtgctatggt
     4561 gcctctggcc cagccacctg ctccagcccc tgtgctgacc ccaggaccac cccagtcact
     4621 gagcgctcca gtgcccaagt ctacacaggc cggcgagggg actctgagtg aagctctgct
     4681 gcacctgcag ttcgacgctg atgaggacct gggagctctg ctggggaaca gcaccgatcc
     4741 cggagtgttc acagatctgg cctccgtgga caactctgag tttcagcagc tgctgaatca
     4801 gggcgtgtcc atgtctcata gtacagccga accaatgctg atggagtacc ccgaagccat
     4861 tacccggctg gtgaccggca gccagcggcc ccccgacccc gctccaactc ccctgggaac
     4921 cagcggcctg cctaatgggc tgtccggaga tgaagacttc tcaagcatcg ctgatatgga
     4981 ctttagtgcc ctgctgtcac agatttcctc tagtgggcag ggaggaggtg gaagcggctt
     5041 cagcgtggac accagtgccc tgctggacct gttcagcccc tcggtgaccg tgcccgacat
     5101 gagcctgcct gaccttgaca gcagcctggc cagtatccaa gagctcctgt ctccccagga
     5161 gccccccagg cctcccgagg cagagaacag cagcccggat tcagggaagc agctggtgca
     5221 ctacacagcg cagccgctgt tcctgctgga ccccggctcc gtggacaccg ggagcaacga
     5281 cctgccggtg ctgtttgagc tgggagaggg ctcctacttc tccgaagggg acggcttcgc
     5341 cgaggacccc accatctccc tgctgacagg ctcggagcct cccaaagcca aggaccccac
     5401 tgtctccgct agcggcagtg gagagggcag aggaagtctg ctaacatgcg gtgacgtcga
     5461 ggagaatcct ggcccagtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     5521 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     5581 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     5641 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     5701 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     5761 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     5821 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     5881 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     5941 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     6001 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     6061 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     6121 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     6181 gctgtacaag taagaattcg tcgagggacc taataacttc gtatagcata cattatacga
     6241 agttatacat gtttaagggt tccggttcca ctaggtacaa ttcgatatca agcttatcga
     6301 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6361 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6421 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6481 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6541 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6601 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6661 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     6721 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6781 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6841 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     6901 tcgacctcga tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     6961 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     7021 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7081 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     7141 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     7201 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     7261 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     7321 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     7381 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     7441 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7501 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7561 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catgtgagca aaaggccagc
     7621 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7681 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7741 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7801 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7861 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7921 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7981 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8041 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8101 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8161 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8221 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8281 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8341 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8401 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8461 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8521 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8581 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8641 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8701 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8761 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8821 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8881 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8941 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9001 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9061 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9121 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9181 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9241 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9301 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
//
