LOCUS       sequence-391933-        6966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  visualization of TdIF2/ERBP as a fusion to mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Fukada T, Shibata S, Ueda T, Sasaki K, Shimoida Y, Senda-Murata K, 
            Sugimoto K
  TITLE     Characterization of nucleolar localization and exclusion signals in 
            terminal deoxynucleotidyltransferase interacting factor 2/estrogen 
            receptor alpha-binding protein.
  JOURNAL   Biosci Biotechnol Biochem. 2019 Jul;83(7):1255-1262. doi: 
            10.1080/09168451.2019.1591265. Epub 2019 Mar 24.
  PUBMED    30907250
REFERENCE   2  (bases 1 to 6966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/09168451.2019.1591265"; journalName: "Biosci Biotechnol 
            Biochem"; date: "2019-07"; volume: "83"; issue: "7"; pages: 
            "1255-1262"
COMMENT     Sequence Label: pmCherry-TdIF2
FEATURES             Location/Qualifiers
     source          1..6966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      598..607
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             604..1311
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(749..767)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1017..1036
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3754..3875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3791..3810)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3845..3864
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3882..4337)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4019..4040)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4231..4250
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4364..4468
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4470..4827
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4470..4661
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4493..4513)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4678..4813
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4862..5656
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4862..5656
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4916..4935)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5526..5545
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5844..5863)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5888..5935
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6264..6852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6753..6772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccgcc
      601 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
      661 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      721 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
      781 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
      841 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
      901 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
      961 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     1021 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     1081 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     1141 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     1201 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     1261 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gagatctcga
     1321 gctcaagctt acatggtggt taccagatct gcacgggcta aggccagcat ccaagccgcg
     1381 tcggctgaaa gttccgggca aaagagtttt gctgctaatg ggattcaagc gcatccagaa
     1441 agtagtactg gatctgatgc ccgaactact gctgaatcac agaccactgg gaagcaaagt
     1501 ttaatcccta gaactcctaa agctagaaag aggaagagca gaactacagg ctcactacca
     1561 aaggggactg aaccatctac ggatggagaa acctctgagg cagagtcaaa ttattctgtg
     1621 tctgagcacc atgataccat tttaagggta actaggagaa ggcagatctt aattgcatgc
     1681 tccccagtgt ccagtgttag gaaaaagccg aaagtaactc caacaaagga gtcttacact
     1741 gaagaaatag tgtctgaagc agaatctcat gtttcaggta tttctagaat tgtgcttcct
     1801 acagaaaaaa ctacaggagc cagaagaagt aaggctaaat ctctgacaga tccaagccaa
     1861 gaatctcata cagaagctat atctgatgct gagacatcaa gctcagacat ttcattctct
     1921 ggaattgcaa ctagaagaac caggagtatg cagaggaaat taaaggcaca aactgaaaag
     1981 aaagatagta agattgtacc aggaaatgag aaacagatcg tgggtacacc tgtgaattca
     2041 gaggattcag ataccagaca aacttcccat ttacaagcaa gatctctttc tgagataaat
     2101 aagccaaatt tctataataa tgactttgat gatgatttct cccacagaag ttcagaaaat
     2161 atattaacag tgcacgaaca ggccaatgtt gaatctctta aagaaacaaa acagaattgt
     2221 aaggatttgg atgaagatgc caatggaata acagatgagg ggaaagaaat taatgagaaa
     2281 agttctcagc tgaagaatct ttctgaactt caggacacta gccttcaaca gttagtttct
     2341 cagagacatt caacccccca aaataaaaat gctgtatcag tgcactctaa tctgaactct
     2401 gaggctgtaa tgaaatcatt aactcaaaca tttgcaactg tggaagtagg cagatggaat
     2461 aacaacaaaa agagccccat aaaagcaagt gacttgacaa agtttggtga ttgtggtggt
     2521 agtgatgatg aagaagagtc cacagttata agtgtcagtg aagacatgaa cagtgaaggg
     2581 aatgtagatt ttgaatgtga taccaaacta tacacgtctg cgcccaacac atctcagggt
     2641 aaagataatt ctgtcttact agttctcagc agtgatgaaa gccaacagtc tgaaaacagt
     2701 gagaatgaag aggatacttt atgttttgtt gaaaatagtg gccaaaggga gtcattaagt
     2761 ggagacacag gaagtctgtc atgtgacaat gcattgtttg taattgacac aactcctgga
     2821 atgagtgctg ataaaaattt ttacttggaa gaggaagaca aggcaagtga ggttgccatt
     2881 gaggaagaaa aagaagagga agaggatgaa aaaagtgaag aagattcatc agaccatgac
     2941 gaaaatgaag atgagtttag tgatgaagaa gacttcctaa atagcacaaa ggctaaactt
     3001 ctgaagttga caagcagcag catagaccct ggtctgagta tcaagcagtt gggtggtttg
     3061 tatattaatt ttaatgcaga taaactacag tctaacaaga gaaccctaac acagatcaag
     3121 gagaaaaaga aaaatgagct tctgcagaaa gccgtcatta cacctgattt tgaaaaaaac
     3181 cactgtgttc caccatatag tgaatcaaag tatcaacttc agaaaaaacg cagaaaagaa
     3241 cgacaaaaaa cagcagggga tggctggttt ggtatgaaag ctccagaaat gacaaatgaa
     3301 ctgaaaaatg atctcaaagc actgaagatg agagccagca tggacccgaa aagattttac
     3361 aagaaaaatg atagagatgg cttccccaag tacttccaga ttggaaccat tgttgacaat
     3421 ccagctgatt tctaccattc acgaattccc aagaagcaaa ggaaaagaac tattgtggaa
     3481 gaactgctgg ctgattctga attcagaaga tacaaccgaa ggaagtactc agagatcatg
     3541 gctgaaaaag cagcaaatgc agcaggaaaa aagttccgaa agaagaagaa atttcgcaat
     3601 taagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc
     3661 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     3721 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     3781 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3841 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
     3901 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     3961 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     4021 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     4081 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     4141 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     4201 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     4261 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4321 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4381 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4441 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     4501 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4561 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4621 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4681 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4741 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4801 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     4861 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4921 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4981 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5041 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5101 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5161 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5221 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5281 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5341 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     5401 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5461 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5521 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5581 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5641 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5701 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5761 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5821 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     5881 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     5941 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     6001 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     6061 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     6121 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6181 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6241 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6301 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6361 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6421 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6481 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6541 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6601 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6661 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6721 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6781 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6841 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6901 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6961 atgcat
//
