LOCUS       sequence-391938-        1794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for glutamate, third generation, variant 857 in
            GPI backbone. iGluSnFR3.v857.GPI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Aggarwal A, Liu R, Chen Y, Ralowicz AJ, Bergerson SJ, Tomaska F, 
            Mohar B, Hanson TL, Hasseman JP, Reep D, Tsegaye G, Yao P, Ji X, 
            Kloos M, Walpita D, Patel R, Mohr MA, Tillberg PW, Looger LL, Marvin
            JS, Hoppa MB, Konnerth A, Kleinfeld D, Schreiter ER, Podgorski K
  TITLE     Glutamate indicators with improved activation kinetics and 
            localization for imaging synaptic transmission.
  JOURNAL   Nat Methods. 2023 May 4. doi: 10.1038/s41592-023-01863-6.
  PUBMED    37142767
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 May 4. doi: 10.1038/s41592-023-01863-6."
COMMENT     Sequence Label: pAAV.CAG.FLEX.iGluSnFR3.v857.GPI
FEATURES             Location/Qualifiers
     source          1..1794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             1645..1674
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacagatctg ggtccgccgc cggaagtact cttgataaga tcgctaaaaa tggtgtaatt
      121 gttgtcggcc accgagactc ttctgtgcca ttcagctatt atgattctca acacaaagtg
      181 gtgggctatt cacaagagta ttctaacgcc attgttgaag cagttaagaa gaagttgaat
      241 aaaccagacc ttcaagtaaa actcattcct ataacatccc aaaaccgcat acctctcttg
      301 caaaacggca cttacgattt cgagtgtggg agcactacta ataatgtcga acgccagaaa
      361 caagccgcat tctccgacac catcttcgtg gtgggcactc gactgttgac aaagaaaggc
      421 ggtgatatta aggatttcgc aaatctgaag gataaggcag tcgtggtcac cagcgggacc
      481 acctcagaag tactccttaa taaactcaat gaagaacaaa agatgaatat gcgaatcatc
      541 tctgctaagg accacggtga ctcttttcgc acattggaaa gtgggcgcgc cgtagcattt
      601 atgatggacg actctctgct cgctggcgag cgcgccaaag ccaaaaaacc agacgactgg
      661 gaaattgtag gtaaacctca aagccaagag gcatatggct gcatgttgag aaaagatgac
      721 ccccaattca agaaactgat ggacgacact atagctcagg tccagacttc aggggaagct
      781 gagaagtggt ttgataaatg gttcaagaac ccaatcttgg taaatcatac cgtttatatt
      841 accgctgaca aacaaaagaa tgggataaag gcaaatttca agattcacca taacgtagag
      901 gatggaagcg tgcagttggc agaccattat caacagaata cccctattgg cgacggaccc
      961 gtgctcctcc ctgacaacca ctacttgtcc tatcaaagtg ttctctcaaa ggaccccaat
     1021 gagaagcgag accacatggt acttctcgaa ttcgttaccg ccaccgggaa ttcacttggc
     1081 atggatgaat tgtataaggg gggtactggc ggcagcatgt ccaagggtga ggagcttttt
     1141 accggagtag taccaattct cgttgaactt gacggagacg tcaacgggca caagtttagc
     1201 gtacgaggtg agggggaggg tgatgcaacc aacggtaaac ttactctcaa attcatatgc
     1261 actaccggaa agctgccagt accttggcca acactggtca ctaccctctc ctatggcgtt
     1321 caatgttttg ctcgataccc tgaccacatg aaacagcatg acttctttaa atcagccatg
     1381 cctgaagggt atgtccagga gcggactata tcatttaaag acgatggaac ctataagact
     1441 cgggctgaag tgaagctcga gggtgacaca ttggttaatc ggatcgagct taagggcata
     1501 gattttaaag aagatggtaa tattcttggc cacaaattgg agtacaattt taataatcct
     1561 ttgaacatga actttgagct ctccgatgaa atgaaggcac tctttaaaga gccaaacgac
     1621 aaagctctga aactgcaggt cgacgaacaa aaactcatct cagaagagga tctgtcagat
     1681 aacgactacg gtaaagatgg gttggctggg gcttcaggag gcagacgaaa tggcgctata
     1741 actgtgctca gtttcataac gttctacgtg gcagcattta tggtattgtt gtag
//
