LOCUS       sequence-39195-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pCS2-F1-GCG
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             17..37
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             38..127
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 atctgccacc atggctccaa agaagaagcg taaggtatac ccatacgatg ttcctgacta
       61 tgcgggctat ccctatgacg tcccggacta tgcaggatcg tatccatatg acgttccaga
      121 ttacgctgct catggtaccc gcccatatgc ttgccctgtc gagtcctgtg accgccgctt
      181 cagtcgctct gacgacctca cccgtcacat ccggattcac acaggccaga agcccttcca
      241 gtgtcgcatc tgcatgcgta acttcagtcg tagtgacgac cttacccgtc acatccgcac
      301 ccacacaggc gagaagcctt ttgcatgcga catctgcggc aggaagttcg cgcgttctga
      361 cgctctcacc cgccacacca agatccacac aggtggatcc caactagtca aaagtgaact
      421 ggaggagaag aaatctgaac ttcgtcataa attgaaatat gtgcctcatg aatatattga
      481 attaattgaa attgccagaa attccactca ggatagaatt cttgaaatga aggtaatgga
      541 attttttatg aaagtttatg gatatagagg taaacatttg ggtggatcaa ggaaaccgga
      601 cggagcaatt tatactgtcg gatctcctat tgattacggt gtgatcgtgg atactaaagc
      661 ttatagcgga ggttataatc tgccaattgg ccaagcacgt gaaatgcaac gatatgtcga
      721 agaaaatcaa acacgaaaca aacatatcaa ccctaatgaa tggtggaaag tctatccatc
      781 ttctgtaacg gaatttaagt ttttatttgt gagtggtcac tttaaaggaa actacaaagc
      841 tcagcttaca cgattaaatc atatcact
//
