LOCUS       sequence-392004-        9230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  scCloneSelect C-to-T DNA barcode cloning backbone (CMV promoter 
            version).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9230)
  AUTHORS   Ishiguro S, Ishida K, Sakata RC, Ichiraku M, Takimoto R, Yogo R, 
            Kijima Y, Mori H, Tanaka M, King S, Tarumoto S, Tsujimura T, Bashth 
            O, Masuyama N, Adel A, Toyoshima H, Seki M, Oh JH, Archambault AS, 
            Nishida K, Kondo A, Kuhara S, Aburatani H, Klein Geltink RI, 
            Yamamoto T, Shakiba N, Takashima Y, Yachie N
  TITLE     A multi-kingdom genetic barcoding system for precise clone 
            isolation.
  JOURNAL   Nat Biotechnol. 2025 May 21. doi: 10.1038/s41587-025-02649-1.
  PUBMED    40399693
REFERENCE   2  (bases 1 to 9230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 May 21. doi: 10.1038/s41587-025-02649-1."
COMMENT     Sequence Label: pRS138
FEATURES             Location/Qualifiers
     source          1..9230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(114..218)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    266..400
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(303..322)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     357..376
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             568..1201
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1248..1373
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1870..2103
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2288..2332
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2595..2710
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2761..3064
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3065..3268
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3219..3239
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3265..3289
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(3434..3455)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        3695..4194
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3730..3753)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4127..4146
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4214..4813
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4214..4233)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4710..4730
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4827..5415
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4880..4900)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5298..5309)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5622..5855
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5984..6000)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5984..6000)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5997..6019)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6008..6024
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6032..6038,6039..6056,6057..6062))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6077..6098
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6215..6232)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6386..6974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6466..6485)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8483..9230,1..44,45..113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9106..9125
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
       61 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
      121 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      181 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      241 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
      301 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
      361 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
      421 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
      481 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
      541 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
      601 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
      661 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
      721 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
      781 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
      841 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
      901 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
      961 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     1021 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     1081 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     1141 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     1201 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     1261 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     1321 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     1381 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     1441 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     1501 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     1561 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     1621 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     1681 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     1741 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     1801 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     1861 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     1921 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     1981 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     2041 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     2101 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     2161 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     2221 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     2281 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     2341 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     2401 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     2461 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     2521 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     2581 tcgacggtat cgcctttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2641 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2701 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttatcga tggagttccg
     2761 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     2821 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     2881 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     2941 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3001 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     3061 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     3121 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     3181 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     3241 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     3301 ccatccacgc tgttttgacc tccatagaag acaccgactc tactagagga tctatttccg
     3361 gtgaattccc gagcgtgtca gggtgaccat ggtgagcaag ggcgaggagc tgttcaccgg
     3421 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     3481 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     3541 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     3601 cttcagccgc taccccgacc acatgaagct ctagagtatt accatggtca aaaaaaaaaa
     3661 aaaaaaaaaa aaaaaaaaag gatccaattc taccgggtag gggaggcgct tttcccaagg
     3721 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     3781 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     3841 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     3901 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     3961 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     4021 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     4081 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     4141 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     4201 agttaacgcc gccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     4261 cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     4321 cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     4381 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     4441 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     4501 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     4561 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     4621 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     4681 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     4741 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     4801 gcccggtgcc tagacgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat
     4861 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4921 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4981 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     5041 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     5101 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5161 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5221 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     5281 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5341 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5401 gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat
     5461 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     5521 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     5581 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc
     5641 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5701 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5761 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5821 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     5881 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta
     5941 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     6001 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6061 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6121 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6181 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6241 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6301 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6361 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6421 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6481 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6541 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6601 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6661 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6721 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6781 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6841 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6901 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6961 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7021 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7081 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtac tgagagatcc cctcataatt
     7141 tccccaaagc gtaaccatgt gtgaataaat tttgagctag tagggttgca gccacgagta
     7201 agtcttccct tgttattgtg tagccagaat gccgcaaaac ttccatgcct aagcgaactg
     7261 ttgagagtac gtttcgattt ctgactgtgt tagcctggaa gtgcttgtcc caaccttgtt
     7321 tctgagcatg aacgcccgca agccaacatg ttagttgaag catcagggcg attagcagca
     7381 tgatatcaaa acgctctgag ctgctcgttc ggctatggcg taggcctagt ccgtaggcag
     7441 gacttttcaa gtctcggaag gtttcttcaa tctgcattcg cttcgaatag atattaacaa
     7501 gttgtttggg tgttcgaatt tcaacaggta agttagttgc tagaacccat ggctcctttg
     7561 ccgacgctga gtagatttta ggtgacgggt ggtgacaatg agtccgtgtc gagcgctgat
     7621 tttttcggcc tttagagcga gatttataca atagaatttg gcatgagatt ggattgcttt
     7681 tagtcagcct cttatagcct aaagtctttg agtgactaga tgacatatca tgtaagttgc
     7741 tgataggttt ccagttttcc gctcctaggt ctgcatattg tacttttcct cttactcgac
     7801 ttaaccagta ccaacccagc ttctcaacgg atttatacca tggcacttta aagccagcat
     7861 cactgacaat gagcggtgtg gtgttactcg gtagaatgct cgcaaggtcg gctagaaatt
     7921 ggtcatgagc tttctttgaa cattgctctg aaagcgggaa cgctttctca taaagagtaa
     7981 cagaacgacc gtgtagtgcg actgaagctc gcaataccat aagtcgtttt tgctcacgaa
     8041 tatcagacca gtcaacaagt acaatgggca tcgtattgcc cgaacagata aagctagcat
     8101 gccaacggta tacagcgagt cgctctttgt ggaggtgacg attacctaac aatcggtcga
     8161 ttcgtttgat gttatgtttt gttctcgctt tggttggcag gttacggcca agttcggtaa
     8221 gagtgagagt tttacagtca agtaatgcgt ggcaagccaa cgttaagctg ttgagtcgtt
     8281 ttaagtgtaa ttcggggcag aattggtaaa gagagtcgtg taaaatatcg agttcgcaca
     8341 tcttgttgtc tgattattga tttttcgcga aaccatttga tcatatgaca agatgtgtat
     8401 ccaccttaac ttaatgattt ttaccaaaat cattagggga ttcatcagtc taaagtatat
     8461 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8521 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8581 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8641 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8701 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8761 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8821 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8881 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8941 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9001 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9061 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9121 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9181 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
//
