LOCUS       sequence-392024-        9739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  scCloneSelect C-to-T reporter encoding BC-C1 barcode (CMV promoter 
            version).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Ishiguro S, Ishida K, Sakata RC, Ichiraku M, Takimoto R, Yogo R, 
            Kijima Y, Mori H, Tanaka M, King S, Tarumoto S, Tsujimura T, Bashth 
            O, Masuyama N, Adel A, Toyoshima H, Seki M, Oh JH, Archambault AS, 
            Nishida K, Kondo A, Kuhara S, Aburatani H, Klein Geltink RI, 
            Yamamoto T, Shakiba N, Takashima Y, Yachie N
  TITLE     A multi-kingdom genetic barcoding system for precise clone 
            isolation.
  JOURNAL   Nat Biotechnol. 2025 May 21. doi: 10.1038/s41587-025-02649-1.
  PUBMED    40399693
REFERENCE   2  (bases 1 to 9739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 May 21. doi: 10.1038/s41587-025-02649-1."
COMMENT     Sequence Label: pRS140
FEATURES             Location/Qualifiers
     source          1..9739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(114..218)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    266..400
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(303..322)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     357..376
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             568..1201
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1248..1373
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1870..2103
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2288..2332
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2595..2710
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2761..3064
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3065..3268
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3219..3239
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3265..3289
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3392..3394,3395..4108)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3392..3394,3395..4105)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3434..3455)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3695..3714)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4042..4063
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4204..4703
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4239..4262)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4636..4655
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4723..5322
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4723..4742)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5219..5239
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5336..5924
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5389..5409)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5807..5818)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6131..6364
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6493..6509)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6493..6509)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6506..6528)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6517..6533
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6541..6547,6548..6565,6566..6571))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6586..6607
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6724..6741)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6895..7483)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6975..6994)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8992..9739,1..44,45..113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9615..9634
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
       61 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
      121 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      181 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      241 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
      301 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
      361 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
      421 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
      481 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
      541 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
      601 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
      661 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
      721 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
      781 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
      841 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
      901 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
      961 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     1021 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     1081 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     1141 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     1201 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     1261 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     1321 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     1381 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     1441 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     1501 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     1561 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     1621 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     1681 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     1741 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     1801 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     1861 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     1921 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     1981 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     2041 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     2101 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     2161 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     2221 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     2281 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     2341 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     2401 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     2461 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     2521 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     2581 tcgacggtat cgcctttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2641 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2701 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttatcga tggagttccg
     2761 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     2821 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     2881 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     2941 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3001 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     3061 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     3121 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     3181 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     3241 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     3301 ccatccacgc tgttttgacc tccatagaag acaccgactc tactagagga tctatttccg
     3361 gtgaattccc gagcgtgtca gggtgaccgt ggtgagcaag ggcgaggagc tgttcaccgg
     3421 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     3481 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     3541 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     3601 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     3661 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     3721 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     3781 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     3841 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     3901 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     3961 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     4021 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     4081 tctcggcatg gacgagctgt acaagtaaag agcgtaaagg acaaaaccca ccacggagtc
     4141 tagagtatta ccatggtcaa aaaaaaaaaa aaaaaaaaaa aaaaaaaagg atccaattct
     4201 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     4261 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     4321 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     4381 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     4441 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     4501 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     4561 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     4621 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     4681 cttcctcatc tccgggcctt tcgacctgca gttaacgccg ccatgaccga gtacaagccc
     4741 acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg
     4801 ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc
     4861 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     4921 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     4981 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     5041 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     5101 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     5161 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     5221 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     5281 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct agacgcgtct ggaacaatca
     5341 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5401 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5461 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5521 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5581 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     5641 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     5701 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     5761 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     5821 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     5881 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc
     5941 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     6001 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     6061 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6121 aagaggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     6181 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6241 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6301 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6361 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     6421 tcagagagtg agaggccttg acattgctag cgttttaccg tcgacctcta gctagagctt
     6481 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6541 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6601 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6661 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6721 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6781 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6841 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6901 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6961 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7021 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7081 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7141 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7201 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7261 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7321 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7381 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7441 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7501 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7561 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7621 ctaaagtact gagagatccc ctcataattt ccccaaagcg taaccatgtg tgaataaatt
     7681 ttgagctagt agggttgcag ccacgagtaa gtcttccctt gttattgtgt agccagaatg
     7741 ccgcaaaact tccatgccta agcgaactgt tgagagtacg tttcgatttc tgactgtgtt
     7801 agcctggaag tgcttgtccc aaccttgttt ctgagcatga acgcccgcaa gccaacatgt
     7861 tagttgaagc atcagggcga ttagcagcat gatatcaaaa cgctctgagc tgctcgttcg
     7921 gctatggcgt aggcctagtc cgtaggcagg acttttcaag tctcggaagg tttcttcaat
     7981 ctgcattcgc ttcgaataga tattaacaag ttgtttgggt gttcgaattt caacaggtaa
     8041 gttagttgct agaacccatg gctcctttgc cgacgctgag tagattttag gtgacgggtg
     8101 gtgacaatga gtccgtgtcg agcgctgatt ttttcggcct ttagagcgag atttatacaa
     8161 tagaatttgg catgagattg gattgctttt agtcagcctc ttatagccta aagtctttga
     8221 gtgactagat gacatatcat gtaagttgct gataggtttc cagttttccg ctcctaggtc
     8281 tgcatattgt acttttcctc ttactcgact taaccagtac caacccagct tctcaacgga
     8341 tttataccat ggcactttaa agccagcatc actgacaatg agcggtgtgg tgttactcgg
     8401 tagaatgctc gcaaggtcgg ctagaaattg gtcatgagct ttctttgaac attgctctga
     8461 aagcgggaac gctttctcat aaagagtaac agaacgaccg tgtagtgcga ctgaagctcg
     8521 caataccata agtcgttttt gctcacgaat atcagaccag tcaacaagta caatgggcat
     8581 cgtattgccc gaacagataa agctagcatg ccaacggtat acagcgagtc gctctttgtg
     8641 gaggtgacga ttacctaaca atcggtcgat tcgtttgatg ttatgttttg ttctcgcttt
     8701 ggttggcagg ttacggccaa gttcggtaag agtgagagtt ttacagtcaa gtaatgcgtg
     8761 gcaagccaac gttaagctgt tgagtcgttt taagtgtaat tcggggcaga attggtaaag
     8821 agagtcgtgt aaaatatcga gttcgcacat cttgttgtct gattattgat ttttcgcgaa
     8881 accatttgat catatgacaa gatgtgtatc caccttaact taatgatttt taccaaaatc
     8941 attaggggat tcatcagtct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9001 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9061 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9121 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9181 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9241 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9301 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9361 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9421 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9481 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9541 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9601 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9661 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9721 gtaacccact cgtgcaccc
//
