LOCUS       sequence-392093-        9586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral construct to stably express Lamp1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Shin HR, Citron YR, Wang L, Tribouillard L, Goul CS, Stipp R, 
            Sugasawa Y, Jain A, Samson N, Lim CY, Davis OB, Castaneda-Carpio D, 
            Qian M, Nomura DK, Perera RM, Park E, Covey DF, Laplante M, Evers 
            AS, Zoncu R
  TITLE     Lysosomal GPCR-like protein LYCHOS signals cholesterol sufficiency 
            to mTORC1.
  JOURNAL   Science. 2022 Sep 16;377(6612):1290-1298. doi: 
            10.1126/science.abg6621. Epub 2022 Aug 25.
  PUBMED    36007018
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abg6621"; journalName: "Science"; date: 
            "2022-09-16- 16"; volume: "377"; issue: "6612"; pages: "1290-1298"
COMMENT     Sequence Label: pLJM1-Lamp1 FLAG
FEATURES             Location/Qualifiers
     source          1..9586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        159..1341
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          390..1332
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1289..1309
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1353..1362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1357..1366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1359..1421,1422..2579)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     CDS             2598..2621
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2669..2786
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2835..3345
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3264..3282
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3367..3966
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3367..3386)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3863..3883
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4094..4327
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4399..4520
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4436..4455)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4490..4509
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4560..4695
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4715..4734)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4716..4734)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4744..4761)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4744..4760)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4753..4775)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4902..5357
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4989..5008)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5199..5220
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5383..5487
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5488..5556,5557..6348)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5706..5725)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6519..7107
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7008..7027
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7261..7278
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7395..7416
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7431..7436,7437..7454,7455..7461)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7469..7485
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7474..7496
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7493..7509
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7493..7509
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7528..7548
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7530..7548
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7576..7802
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7803..7983
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8030..8155
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8648..8881
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9066..9110
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     9408..9428
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tacgatgagt aattcataca aaaggactcg cccctgcctt ggggaatccc agggaccgtc
       61 gttaaactcc cactaacgta gaacccagag atcgctgcgt tcccgccccc tcacccgccc
      121 gctctcgtca tcactgaggt ggagaagagc atgcgtgagg ctccggtgcc cgtcagtggg
      181 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
      241 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
      301 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
      361 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
      421 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacgccc ctggctgcag
      481 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
      541 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcttgggcg ctggggccgc
      601 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
      661 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
      721 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
      781 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
      841 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
      901 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
      961 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     1021 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     1081 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     1141 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     1201 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     1261 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     1321 cttccatttc aggtgtcgtg aggatcgcta gcgccaccat ggcggccccg ggcgcccggc
     1381 ggccgctgct cctgttgctg ctggcaggcc ttgcacacag cgccccagca ctgttcgagg
     1441 tgaaagacaa caacggcaca gcgtgtataa tggccagctt ctctgcctcc tttctgacca
     1501 cctatgaggc tggacatgtt tctaaggtct cgaatatgac cctgccagcc tctgcagaag
     1561 tcctgaagaa tagcagctct tgtggtgaaa agaatgcttc tgagcccacc ctcgcaatca
     1621 cctttggaga aggatattta ctgaaactca ccttcacaaa aaacacaaca cgttacagtg
     1681 tccagcacat gtatttcaca tataacctgt cagacacaca attctttccc aatgccagct
     1741 ccaaagggcc cgacactgtg gattccacaa ctgacatcaa ggcagacatc aacaaaacat
     1801 accgatgtgt cagcgacatc agggtctaca tgaagaatgt gaccattgtg ctctgggacg
     1861 ctactatcca ggcctacctg ccgagtagca acttcagcaa ggaagagaca cgctgcccac
     1921 aggatcaacc ttccccaact actgggccac ccagcccctc accaccactt gtgcccacaa
     1981 accccagtgt gtccaagtac aatgtgactg gtgacaatgg aacctgcctg ctggcctcta
     2041 tggcactgca actcaacatc acctacatga agaaggacaa cacgactgtg accagagcat
     2101 tcaacatcaa cccaagtgac aaatatagtg ggacttgcgg tgcccagttg gtgaccctga
     2161 aggtggggaa caagagcaga gtcctggagc tgcagtttgg gatgaatgcc acttctagcc
     2221 tgtttttcct gcaaggagtt cagttgaaca tgactcttcc tgatgccata gagcccacgt
     2281 tcagcacctc caactattcc ctgaaagctc ttcaggccag tgtcggcaac tcatacaagt
     2341 gcaacagtga ggagcacatc tttgtcagca aggcgctcgc cctcaatgtc ttcagcgtgc
     2401 aagtccaggc tttcagggta gaaagtgaca ggtttgggtc tgtggaagag tgtgtacagg
     2461 acggtaacaa catgctgatc cccattgctg tgggcggggc cctggcaggg ctggtcctca
     2521 tcgtcctcat cgcctacctc atcggcagga agaggagtca cgcgggctat cagaccatca
     2581 ccggtggtgg tggtggtgac tacaaggatg acgatgacaa gtaattcgaa ttctcgacct
     2641 cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     2701 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     2761 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
     2821 ggccgcggct cgagggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag
     2881 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc
     2941 acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc
     3001 ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc
     3061 cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag
     3121 ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg
     3181 cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg
     3241 tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg
     3301 gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccaggggga tccaccggag
     3361 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     3421 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     3481 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     3541 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     3601 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     3661 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     3721 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     3781 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     3841 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     3901 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     3961 gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca
     4021 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     4081 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     4141 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4201 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4261 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4321 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     4381 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4441 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4501 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     4561 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4621 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     4681 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     4741 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     4801 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     4861 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     4921 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4981 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5041 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5101 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5161 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5221 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     5281 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     5341 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5401 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5461 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5521 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5581 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5641 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5701 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5761 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5821 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5881 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5941 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6001 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6061 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6121 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6181 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6241 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6301 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6361 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6421 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6481 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6541 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6601 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6661 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6721 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6781 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6841 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6901 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6961 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7021 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7081 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7141 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7201 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7261 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7321 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     7381 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     7441 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     7501 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     7561 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     7621 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     7681 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     7741 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     7801 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7861 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7921 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7981 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     8041 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     8101 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     8161 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     8221 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     8281 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     8341 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     8401 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     8461 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     8521 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     8581 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     8641 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     8701 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     8761 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     8821 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     8881 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     8941 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     9001 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     9061 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     9121 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     9181 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     9241 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     9301 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     9361 gacggtatcg atcacgagac tagcctcgag cggccgcccc cttcaccgag ggcctatttc
     9421 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attggaatta
     9481 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc
     9541 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatca
//
