LOCUS       sequence-392097-        5575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 HCoV-NL63 NSP12_nostop
FEATURES             Location/Qualifiers
     source          1..5575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2886..2985)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3002..3021)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3003..3021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3026..3042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3026..3042)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3473..4264
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4357..4945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4846..4865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5099..5116
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5275..5302)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5394..5480)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5529..5551
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5543..5560
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5544..5560
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgagtgttga catttcttat
      121 ttaaaccgag caaggggttc tagtgcagct cgactagaac cctgcaatgg cacggacatc
      181 gataagtgtg ttcgtgcttt tgacatttat aataaaaatg tttcattctt gggtaagtgt
      241 ttgaagatga actgtgttcg ttttaaaaat gctgatctta aggatggtta ttttgttata
      301 aagaggtgta ctaagtcggt tatggaacac gagcaatcca tgtataacct acttaacttt
      361 tctggtgctt tggctgagca tgatttcttt acttggaaag atggcagagt catttatggt
      421 aatgttagta gacataatct tactaaatat actatgatgg acttggtcta tgctatgcgt
      481 aactttgatg aacaaaattg tgatgttcta aaagaagtat tagttttaac tggttgttgt
      541 gacaattctt attttgatag taagggttgg tatgacccag ttgaaaatga agatatacat
      601 agagtttatg catctcttgg caaaattgta gctagagcta tgcttaaatg cgttgctcta
      661 tgcgatgcga tggttgctaa aggtgttgtt ggtgttttaa cattagataa ccaagatctt
      721 aatggtaact tttatgattt tggtgatttt gttgttagct tacctaatat gggtgttccc
      781 tgttgtacat catattattc ttatatgatg cctattatgg gtttaactaa ttgtttagct
      841 agtgagtgtt ttgtcaagag tgatattttt ggtagtgatt ttaaaacttt tgatttgctt
      901 aagtatgatt tcactgaaca taaagaaaat ttattcaata agtactttaa gcattggagt
      961 tttgattatc atcctaattg tagtgactgt tatgatgata tgtgtgttat acattgtgct
     1021 aattttaata cactatttgc cacaactata ccaggtactg cttttggtcc actatgtcgt
     1081 aaagttttta tagatggtgt tccacttgtt acaactgctg gttatcattt taagcaatta
     1141 ggtttggttt ggaataaaga tgttaacaca cactcagtta ggttgacaat tactgaactt
     1201 ttgcaatttg tcaccgaccc ttccttgata atagcttctt ccccagcact cgttgatcaa
     1261 cgcactattt gtttttctgt tgcagcattg agtactggtt tgacaaatca agttgttaag
     1321 ccaggtcatt ttaatgaaga gttttataac tttcttcgtt taagaggttt ctttgatgaa
     1381 ggttctgaac ttacattaaa acatttcttc ttcgcacaga atggtgatgc tgctgttaaa
     1441 gattttgact tttaccgtta taataagcct accattttag atatttgtca agctagagtt
     1501 acatataaga tagtctctcg ttattttgac atttatgaag gtggctgtat taaggcatgt
     1561 gaagttgttg taacaaatct taataagagt gctggttggc cattaaataa gtttggtaaa
     1621 gctagtttgt attatgaatc tatatcttat gaagaacagg atgctttgtt tgctttgaca
     1681 aagcgtaatg tcctccctac tatgacacag ctgaatctta agtatgctat tagtggtaaa
     1741 gaacgtgcta gaactgttgg tggtgtttct ctgttgtcta caatgaccac aagacaatac
     1801 catcaaaaac atcttaaatc cattgttaat acacgcaatg ccactgttgt tattggtact
     1861 accaaatttt atggtggttg gaataatatg ttgcgtactt taattgatgg tgttgaaaac
     1921 cctatgctta tgggttggga ttatcccaaa tgtgatagag ctttgcctaa catgatacgt
     1981 atgatttcag ccatggtgtt gggctctaag catgttaatt gttgtactgc aacagatagg
     2041 ttttataggc ttggtaatga gttggcacaa gttttaacag aagttgttta ttctaatggt
     2101 ggtttttatt ttaagccagg tggtacgact tctggtgacg ctagtacagc ttatgctaat
     2161 tctattttta acatttttca agccgtgagt tctaacatta acaggttgct tagtgtccca
     2221 tcagattcat gtaataatgt taatgttagg gatctacaac gacgtctgta tgataattgt
     2281 tataggttaa ctagtgttga agagtcattc attgatgatt attatggtta tcttaggaaa
     2341 catttttcaa tgatgattct ctctgatgac ggtgttgtct gttataacaa ggattatgct
     2401 gagttaggtt atatagcaga cattagtgct tttaaagcca ctttgtatta ccagaataat
     2461 gtctttatga gtacttctaa atgttgggtt gaagaagatt taactaaggg accacatgag
     2521 ttttgttccc agcatactat gcaaatagtt gacaaagatg gtacctatta tttgccttac
     2581 ccagatccta gtaggatctt gtcagctggt gtttttgttg atgatgttgt taagacagat
     2641 gctgttgttt tgttagaacg ttatgtgtct ttagctattg atgcataccc tctttcaaaa
     2701 caccctaatt ccgaatatcg taaggttttt tacgtattac ttgattgggt taagcatctt
     2761 aacaaaaatt tgaatgaggg tgttcttgaa tctttttctg ttacacttct tgataatcaa
     2821 gaagataagt tttggtgtga agatttttat gctagtatgt atgaaaattc tacaatattg
     2881 caagtaccca actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
     2941 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
     3001 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     3061 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac
     3121 cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc
     3181 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
     3241 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     3301 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     3361 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     3421 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga
     3481 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     3541 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     3601 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     3661 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     3721 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     3781 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     3841 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     3901 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     3961 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     4021 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     4081 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     4141 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     4201 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     4261 ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     4321 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4381 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4441 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4501 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4561 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4621 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4681 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4741 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4801 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4861 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4921 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4981 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     5041 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     5101 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     5161 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     5221 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
     5281 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
     5341 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
     5401 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac
     5461 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg
     5521 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
