LOCUS       sequence-392105-       12418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral construct to stably express LYCHOS (GPR155) TurboID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12418)
  AUTHORS   Shin HR, Citron YR, Wang L, Tribouillard L, Goul CS, Stipp R, 
            Sugasawa Y, Jain A, Samson N, Lim CY, Davis OB, Castaneda-Carpio D, 
            Qian M, Nomura DK, Perera RM, Park E, Covey DF, Laplante M, Evers 
            AS, Zoncu R
  TITLE     Lysosomal GPCR-like protein LYCHOS signals cholesterol sufficiency 
            to mTORC1.
  JOURNAL   Science. 2022 Sep 16;377(6612):1290-1298. doi: 
            10.1126/science.abg6621. Epub 2022 Aug 25.
  PUBMED    36007018
REFERENCE   2  (bases 1 to 12418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abg6621"; journalName: "Science"; date: 
            "2022-09-16- 16"; volume: "377"; issue: "6612"; pages: "1290-1298"
COMMENT     Sequence Label: pLVX LYCHOS-FLAG-TiD
FEATURES             Location/Qualifiers
     source          1..12418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    673..798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1295..1528
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1713..1757
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2020..2135
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2329..3511
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2560..3502
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3459..3479
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             complement(5467..5478)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             6236..7192
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             7193..7216
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        7245..7744
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7280..7303)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7677..7696
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7765..8364
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7765..7784)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8261..8281
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8378..8966
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8431..8451)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8849..8860)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9173..9806
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9935..9951)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9935..9951)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9948..9970)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9959..9975
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9983..9989,9990..10007,10008..10013))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10028..10049
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10166..10183)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10337..10925)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10417..10436)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11096..11887,11888..11956))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11957..12061)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12109..12243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12146..12165)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12200..12219
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(12411..12418,1..626)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
       61 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
      121 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
      181 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
      241 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
      301 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
      361 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
      421 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
      481 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
      541 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
      601 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
      661 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
      721 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
      781 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
      841 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
      901 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
      961 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     1021 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     1081 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     1141 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1201 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1261 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1321 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1381 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1441 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1501 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1561 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     1621 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     1681 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     1741 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     1801 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     1861 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     1921 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     1981 gatccattcg attagtgaac ggatctcgac ggtatcgcct ttaaaagaaa aggggggatt
     2041 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2101 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2161 gatccagttt atcgatgagt aattcataca aaaggactcg cccctgcctt ggggaatccc
     2221 agggaccgtc gttaaactcc cactaacgta gaacccagag atcgctgcgt tcccgccccc
     2281 tcacccgccc gctctcgtca tcactgaggt ggagaagagc atgcgtgagg ctccggtgcc
     2341 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     2401 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     2461 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     2521 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc
     2581 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacgccc
     2641 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
     2701 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcttgggcg
     2761 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
     2821 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     2881 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     2941 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     3001 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     3061 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     3121 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     3181 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     3241 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     3301 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     3361 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     3421 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     3481 aagttttttt cttccatttc aggtgtcgtg aggatcgcta gcgctaccgg actcagatct
     3541 cgagctcaag cttcgaattg gatccgccac catgaattct aatttacctg cagagaactt
     3601 aaccattgca gtcaatatga ccaagacttt gcctacagca gtaacgcatg gatttaattc
     3661 cactaatgac ccaccttcaa tgtcaattac aaggcttttt ccagccttac tggaatgctt
     3721 tggcattgtc ctttgtggct acatagcagg aagggccaat gtcataacat caacccaggc
     3781 caaaggacta ggaaattttg tctccagatt tgcacttcca gctttattat tcaaaaacat
     3841 ggttgtactt aatttttcca atgtggactg gtccttccta tatagtatct taattgccaa
     3901 agcttctgta tttttcattg tatgtgtatt aaccttattg gttgccagtc ctgatagtcg
     3961 atttagcaaa gctggactat tccctatttt tgctacacaa agtaatgact ttgcattggg
     4021 ataccctata gttgaagctt tatatcaaac tacataccca gaatatctcc agtacattta
     4081 tttggtggca ccaatatctc ttatgatgtt aaaccctata gggtttattt tctgtgaaat
     4141 ccaaaagtgg aaagacactc aaaatgcttc tcaaaataaa ataaaaattg tgggactcgg
     4201 actcctgcgt gtattacaga acccaatagt atttatggtc ttcattggca tcgccttcaa
     4261 ttttattctt gatcgaaagg tacctgtata tgtcgaaaat tttcttgatg gacttggaaa
     4321 ttctttttct ggatcagccc tattttatct tggtctcacg atggtgggaa aaataaagag
     4381 actgaagaag tcggcatttg tagtactaat tcttctcatc acagctaaac ttctggtgct
     4441 gccacttctg tgcagagaaa tggtggaact cttggacaag ggcgacagtg tggtgaacca
     4501 tacaagttta tcaaattatg catttctgta tggtgtattt cctgtagcac caggagtggc
     4561 tatctttgca acacaattca acatggaagt agaaattata acctcaggga tggtgataag
     4621 cacatttgtg tctgctccca tcatgtacgt ttctgcctgg ttactgacct ttcccactat
     4681 ggaccctaag ccattggcat atgccatcca gaatgttagt tttgatataa gtattgtcag
     4741 cctgatctcc ttgatctggt ctctggctat tcttcttttg agtaagaaat ataaacagct
     4801 tcctcatatg cttacaacta atttactcat tgctcagtct attgtctgtg ctggaatgat
     4861 gatatggaat tttgttaaag aaaaaaattt tgttggacaa attttggtgt ttgttctatt
     4921 gtacagctcc ctctatagca cctacctgtg gacaggcctt ctagcaattt ctttgtttct
     4981 tttgaaaaag cgagagaggg tacaaattcc tgttggaata atcataatat ctggctgggg
     5041 aattcctgct ctccttgttg gtgttctttt gataactgga aaacacaatg gagatagcat
     5101 tgactcagcc ttcttttatg gaaaagaaca gatgatcacc acagcagtca ccctgttctg
     5161 cagcatcctg atagctggca tatccctcat gtgcatgaac cagactgccc aagcaggaag
     5221 ctatgaaggt ttcgatcagt ctcagagcca caaagtggtg gagcctggaa atactgcttt
     5281 tgaggagagt ccagcaccag taaatgaacc agaacttttt acaagctcta ttccagaaac
     5341 aagttgctgc tcctgctcca tgggaaatgg tgaattacac tgcccatcaa tagagccaat
     5401 agcaaacaca agcaccagtg agcctgtgat tccttcgttt gagaaaaaca atcattgtgt
     5461 gagtcgctgt aactcccaga gctgcatatt agcccaggaa gaagaacagt atctacagag
     5521 tggagaccag caactgaccc gacatgtgtt gctgtgttta cttctcatca ttggcctgtt
     5581 cgctaatctt tccagttgtt tatggtggct attcaaccaa gagcctggaa gactttatgt
     5641 tgagttacag tttttctgtg ccgtgtttaa ctttggtcag ggatttattt cctttggaat
     5701 ctttggatta gataaacatt taatcatcct gcctttcaaa agaagacttg aattcctatg
     5761 gaacaataaa gacacagcag aaaacaggga ttctcctgtt tcagaggaaa taaaaatgac
     5821 ctgtcaacaa tttatccatt atcaccgtga cctctgtatc cgaaacattg tcaaagaaag
     5881 aaggtgtggt gcaaagactt ctgctggaac tttctgtggc tgtgacctgg tgagctggct
     5941 aattgaagtc ggccttgcct ccgaccgtgg tgaagctgtg atatacggag acaggctggt
     6001 acaaggggga gtcatccaac atattaccaa tgagtatgaa ttccgggatg agtacttgtt
     6061 ttacagattt cttcaaaaga gtcctgaaca gagtcctcct gctattaatg caaacactct
     6121 ccaacaggaa agatataaag aaattgagca ttcatcccca ccctcacatt cccctaagac
     6181 cggtggtggt gcggccgctg gcggtggtag cggtggcgga agcggtggag gcagtaaaga
     6241 caatactgtg cctctgaagc tgatcgctct cctggctaat ggcgagttcc atagtggcga
     6301 acagctggga gaaaccctgg gcatgtccag ggccgctatc aacaagcaca ttcagactct
     6361 gcgcgactgg ggcgtggacg tgttcaccgt gcccggaaag ggctactctc tgcccgagcc
     6421 tatcccgctg ctgaacgcta aacagattct gggacagctg gacggcggga gcgtggcagt
     6481 cctgcctgtg gtcgactcca ccaatcagta cctgctggat cgaatcggcg agctgaagag
     6541 tggggatgct tgcattgcag aatatcagca ggcagggaga ggaagcagag ggaggaaatg
     6601 gttctctcct tttggagcta acctgtacct gagtatgttt tggcgcctga agcggggacc
     6661 agcagcaatc ggcctgggcc cggtcatcgg aattgtcatg gcagaagcgc tgcgaaagct
     6721 gggagcagac aaggtgcgag tcaaatggcc caatgacctg tatctgcagg atagaaagct
     6781 ggcaggcatc ctggtggagc tggccggaat aacaggcgat gctgcacaga tcgtcattgg
     6841 cgccgggatt aacgtggcta tgaggcgcgt ggaggaaagc gtggtcaatc agggctggat
     6901 cacactgcag gaagcaggga ttaacctgga caggaatact ctggccgcta cgctgatccg
     6961 agagctgcgg gcagccctgg aactgttcga gcaggaaggc ctggctccat atctgccacg
     7021 gtgggagaag ctggataact tcatcaatag acccgtgaag ctgatcattg gggacaaaga
     7081 gattttcggg attagccggg ggattgataa acagggagcc ctgctgctgg aacaggacgg
     7141 agttatcaaa ccctggatgg gcggagaaat cagtctgcgg tctgccgaaa aggactacaa
     7201 ggatgacgat gataaataac ccggctcatc tagataattc taccgggtag gggaggcgct
     7261 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
     7321 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
     7381 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
     7441 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
     7501 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
     7561 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
     7621 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
     7681 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
     7741 ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     7801 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     7861 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     7921 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     7981 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     8041 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     8101 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     8161 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     8221 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     8281 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     8341 atgacccgca agcccggtgc ctgaccgcgt ctggaacaat caacctctgg attacaaaat
     8401 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     8461 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     8521 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     8581 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     8641 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     8701 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     8761 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     8821 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     8881 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     8941 gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa
     9001 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     9061 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     9121 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg
     9181 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     9241 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
     9301 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
     9361 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     9421 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
     9481 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
     9541 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     9601 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     9661 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9721 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9781 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     9841 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgttttac
     9901 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     9961 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    10021 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    10081 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    10141 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    10201 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    10261 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    10321 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    10381 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    10441 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    10501 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    10561 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    10621 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10681 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10741 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    10801 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10861 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10921 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10981 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    11041 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    11101 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    11161 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    11221 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    11281 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    11341 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    11401 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    11461 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    11521 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    11581 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    11641 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    11701 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    11761 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    11821 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    11881 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    11941 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    12001 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    12061 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt
    12121 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    12181 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    12241 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
    12301 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
    12361 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaaggg
//
