LOCUS       sequence-392112-        5577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 HCoV-OC43 NSP12_nostop
FEATURES             Location/Qualifiers
     source          1..5577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2888..2987)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3004..3023)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3005..3023)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3028..3044)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3028..3044)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3475..4266
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4359..4947
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4848..4867
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5101..5118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5277..5304)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5396..5482)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5531..5553
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5545..5562
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5546..5562
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgtcaaaaga tactaatttt
      121 ttaaaccggg ttcggggtac gagtgtagat gcccgtctcg taccctgcgc cagtggttta
      181 tctactgatg tacaattaag ggcatttgat atttacaatg ctagtgttgc tggcattggt
      241 ttacatttaa aagttaattg ttgccgtttt cagcgtgttg atgagaacgg tgataaatta
      301 gatcagttct ttgttgttaa gaggacagat ctgactatat ataatagaga gatgaaatgc
      361 tatgagcgtg taaaagattg taagtttgtg gctgaacacg atttctttac atttgatgta
      421 gaaggtagtc gtgtgccaca cattgtacgc aaggatttaa caaagtatac tatgttggat
      481 ctttgctatg cattgcgaca ttttgatcgc aatgattgca tgctgctttg tgacattctc
      541 tctatatatg ctggttgtga acaatcctac tttactaaga aggattggta tgattttgtt
      601 gaaaatcctg atattattaa tgtgtataaa aagctaggac ctatttttaa tagagcccta
      661 gttagcgcta ctgagtttgc ggacaaattg gtggaggtag gcttagtagg cgttttaaca
      721 cttgataatc aagatttaaa tggtaaatgg tatgattttg gtgactatgt tattgcagcc
      781 ccaggatgtg gtgttgctat agcagattct tattattctt atatcatgcc tatgctgacc
      841 atgtgtcatg cattggattg cgaattgtat gtgaataatg cttatagact atttgatctt
      901 gtacagtatg attttactga ttacaagctt gaattgttta ataagtattt taagcactgg
      961 agtatgccat atcatcctaa cactgttgat tgtcaggatg atcggtgtat tatacattgt
     1021 gctaatttta acatactttt tagtatggtt ttacctaata catgttttgg gcctcttgtt
     1081 aggcaaattt ttgtggatgg tgtgcctttt gttgtttcaa ttggctacca ttataaagaa
     1141 cttggtattg tgatgaatat ggatgtggat acacatcgtt atcgcttgtc tttaaaagac
     1201 ttgcttttat atgctgctga tccagctttg catgtagctt ctgctagtgc attgtatgat
     1261 ttacgcactt gctgttttag tgttgccgct ataacaagcg gtgtaaaatt tcaaacagtt
     1321 aaacctggta attttaatca ggatttttat gattttgttt taagtaaagg cctgcttaaa
     1381 gagggtagct cagttgatct gaagcacttt ttctttacac aggatggtaa tgctgctatt
     1441 actgattata attattataa gtataatttg cccaccatgg tggacattaa gcagttgttg
     1501 tttgttttgg aagttgttta taagtatttt gagatttatg atggtgggtg tataccggca
     1561 tcacaagtca ttgttaataa ttatgataag agtgctggct atccatttaa caaatttgga
     1621 aaagccaggc tctattatga agcattatca tttgaggaac aggatgaaat ttacgcttat
     1681 actaagcgta atgtcctgcc aacacttact caaatgaatt tgaaatatgc tattagtgct
     1741 aagaatagag cccgcactgt tgctggtgtt tccatactta gtactatgac tggcagaatg
     1801 tttcatcaaa aatgtttgaa aagtatagca gctacacgtg gtgttcctgt agttataggc
     1861 accactaaat tttatggtgg ctgggatgat atgttacgcc gccttattaa agatgttgac
     1921 aatcctgtac ttatgggttg ggattatcct aagtgtgatc gtgctatgcc aaacctacta
     1981 cgtattgtta gtagtttggt attagcccga aaacatgaga catgttgttc gcaaagcgat
     2041 aggttttatc gacttgcgaa tgaatgcgca caagttttga gtgaaattgt tatgtgtggt
     2101 ggctgttatt atgttaagcc tggtggcact agtagtggtg atgcaactac tgcttttgct
     2161 aattcagtct ttaacatatg tcaagctgtt tcagccaatg tatgtgcctt aatgtcatgc
     2221 aatggcaata agattgaaga tcttagtata cgtgctcttc agaagcgctt atactcacat
     2281 gtgtatagaa gtgataaggt tgattcaacc tttgtcacag aatattatga atttttaaat
     2341 aagcatttta gtatgatgat tttgagtgat gatggggttg tgtgttataa ttctgattat
     2401 gcgtccaaag ggtatattgc taatataagt gcctttcaac aggtattata ttatcaaaat
     2461 aacgttttta tgtcagaatc caaatgttgg gttgaacatg acataaataa tggacctcat
     2521 gaattctgtt cacaacacac aatgcttgta aagatggatg gtgacgatgt ctaccttcca
     2581 tatcctaatc ctagtcgtat attaggagct ggatgttttg tagatgattt gttaaagact
     2641 gatagtgttc ttttaataga acgatttgta agtcttgcaa tagatgctta tccacttgtg
     2701 tatcatgaaa atgaagaata ccaaaaggtt tttcgtgttt atttggcgta tataaagaag
     2761 ttgtacaatg acctgggtaa tcagatcttg gatagctaca gtgttatttt aagtacttgt
     2821 gatggacaaa agttcactga tgagtccttt tacaagaaca tgtatttaag aagtgcagtt
     2881 atgcagtacc caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     2941 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     3001 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     3061 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag
     3121 accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca
     3181 ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg
     3241 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
     3301 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
     3361 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     3421 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg
     3481 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     3541 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     3601 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     3661 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     3721 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     3781 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     3841 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     3901 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     3961 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     4021 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     4081 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     4141 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     4201 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     4261 aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     4321 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4381 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4441 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4501 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4561 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4621 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4681 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4741 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4801 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4861 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4921 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4981 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5041 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     5101 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     5161 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     5221 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
     5281 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
     5341 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
     5401 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
     5461 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
     5521 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccc
//
