LOCUS       sequence-392115-       13051 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not 
            contain exon 18a, Short form of alternatively spliced C-teminus, SNP
            rs2278973 variant Arginine, in pcDNA3 vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13051)
  AUTHORS   Dahimene S, Page KM, Nieto-Rostro M, Pratt WS, Dolphin AC
  TITLE     The Interplay Between Splicing of Two Exon Combinations 
            Differentially Affects Membrane Targeting and Function of Human 
            CaV2.2.
  JOURNAL   Function (Oxf). 2023 Oct 19;5(1):zqad060. doi: 
            10.1093/function/zqad060. eCollection 2024.
  PUBMED    38020068
REFERENCE   2  (bases 1 to 13051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Function 
            (Oxf)."; date: "2023-10-19"; volume: "5(1):zqad060. doi"; pages: " 
            10.1093/function/zqad060. eCollection 2024"
COMMENT     Sequence Label: GFP Cav2.2 human -HAgHA-minus exon 18a -Short-...
FEATURES             Location/Qualifiers
     source          1..13051
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             973..1686
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             973..1686
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1003..1031)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1626..1646
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             3385..3411
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3415..3441
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(8604..8622)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8605..8622)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8642..8659)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8648..8872
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8918..9346
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9005..9024)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9215..9236
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9355..9375)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9360..9689
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9540..9675
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9602..9621
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9756..10550
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9810..9829)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10420..10439
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10724..10845
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10761..10780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10815..10834
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10894..10910)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10894..10910)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10907..10929)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10918..10934
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10942..10948,10949..10966,10967..10972))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10987..11008
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11125..11142)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11296..11884)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11376..11395)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12055..12846,12847..12915))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12678..12697
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12916..13020)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcctcgagg gtaccgagct
      961 cggatctaaa aaatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     1021 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     1081 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
     1141 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
     1201 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     1261 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     1321 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     1381 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     1441 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     1501 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     1561 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     1621 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     1681 tacaaaatgg tccgcttcgg ggacgagctg ggcggccgct atgggggccc cggcggcgga
     1741 gagcgggccc ggggcggcgg ggccggcggg gcggggggcc cgggtcccgg ggggctgcag
     1801 cccggccagc gggtcctcta caagcaatcg atcgcgcagc gcgcgcggac catggcgctg
     1861 tacaacccca tcccggtcaa gcagaactgc ttcaccgtca accgctcgct cttcgtcttc
     1921 agcgaggaca acgtcgtccg caaatacgcg aagcgcatca ccgagtggcc tccattcgag
     1981 tatatgatcc tggccaccat catcgccaac tgcatcgtgc tggccctgga gcagcacctc
     2041 cctgatgggg acaaaacgcc catgtccgag cggctggacg acacggagcc ctatttcatc
     2101 gggatctttt gcttcgaggc agggatcaaa atcatcgctc tgggctttgt cttccacaag
     2161 ggctcttacc tgcggaacgg ctggaacgtc atggacttcg tggtcgtcct cacagggatc
     2221 cttgccacgg ctggaactga cttcgacctg cgaacactga gggctgtgcg tgtgctgagg
     2281 cccctgaagc tggtgtctgg gattccaagt ttgcaggtgg tgctcaagtc catcatgaag
     2341 gccatggttc cactcctgca gattgggctg cttctcttct ttgccatcct catgtttgcc
     2401 atcattggcc tggagttcta catgggcaag ttccacaagg cctgtttccc caacagcaca
     2461 gatgcggagc ccgtgggtga cttcccctgt ggcaaggagg ccccagcccg gctgtgcgag
     2521 ggcgacactg agtgccggga gtactggcca ggacccaact ttggcatcac caactttgac
     2581 aatatcctgt ttgccatctt gacggtgttc cagtgcatca ccatggaggg ctggactgac
     2641 atcctctata atacaaacga tgcggccggc aacacctgga actggctcta cttcatccct
     2701 ctcatcatca tcggctcctt cttcatgctc aacctggtgc tgggcgtgct ctcgggggag
     2761 tttgccaagg agcgagagag ggtggagaac cgccgcgcct tcctgaagct gcgccggcag
     2821 cagcagatcg agcgagagct caacgggtac ctggagtgga tcttcaaggc ggaggaagtc
     2881 atgctggccg aggaggacag gaatgcagag gagaagtccc ctttggacgc agtgctgaag
     2941 agagcggcca ccaagaagag cagaaatgac ctgatccacg cagaggaggg agaggaccgg
     3001 tttgcagatc tctgtgctgt tggatccccc ttcgcccgcg ccagcctcaa gagcgggaag
     3061 acagagagct cgtcatactt ccggaggaag gagaagatgt tccggttttt tatccggcgc
     3121 atggtgaagg ctcagagctt ctactgggtg gtgctgtgcg tggtggccct gaacacactg
     3181 tgtgtggcca tggtgcatta caaccagccg cggcggctta ccacgaccct gtattttgca
     3241 gagtttgttt tcttgggtct cttcctcaca gagatgtccc tgaagatgta tggcctgggg
     3301 cccagaagct acttccggtc ctccttcaac tgcttcgact ttggggtcat cgtggggagc
     3361 gtctttgaag tggtctgggc ggcctacccc tacgacgtcc ccgactacgc cggctacccg
     3421 tacgacgtcc cggactacgc cggcgtcgac atcaagccgg gaagctcctt tgggatcagt
     3481 gtgctgcggg ccctccgcct gctgaggatc ttcaaagtca cgaagtactg gagctccctg
     3541 cggaacctgg tggtgtccct gctgaactcc atgaagtcca tcatcagcct gctcttcttg
     3601 ctcttcctgt tcattgtggt cttcgccctg ctggggatgc agctgtttgg gggacagttc
     3661 aacttccagg atgagactcc cacaaccaac ttcgacacct tccctgccgc catcctcact
     3721 gtcttccaga tcctgacggg agaggactgg aatgcagtga tgtatcacgg gatcgaatcg
     3781 caaggcggcg tcagcaaagg catgttctcg tccttttact tcattgtcct gacactgttc
     3841 ggaaactaca ctctgctgaa tgtctttctg gccatcgctg tggacaacct ggccaacgcc
     3901 caagagctga ccaaggatga agaggagatg gaagaagcag ccaatcagaa gcttgctctg
     3961 caaaaggcca aagaagtggc tgaagtcagc cccatgtctg ccgcgaacat ctccatcgcc
     4021 gccaggcagc agaactcggc caaggcgcgc tcggtgtggg agcagcgggc cagccagcta
     4081 cggctgcaga acctgcgggc cagctgcgag gcgctgtaca gcgagatgga ccccgaggag
     4141 cggctgcgct tcgccactac gcgccacctg cggcccgaca tgaagacgca cctggaccgg
     4201 ccgctggtgg tggagctggg ccgcgacggc gcgcgggggc ccgtgggagg caaagcccga
     4261 cctgaggctg cggaggcccc cgagggcgtc gaccctccgc gcaggcacca ccggcaccgc
     4321 gacaaggaca agacccccgc ggcgggggac caggaccgag cagaggcccc gaaggcggag
     4381 agcggggagc ccggtgcccg ggaggagcgg ccgcggccgc accgcagcca cagcaaggag
     4441 gccgcggggc ccccggaggc gcggagcgag cgcggccgag gcccaggccc cgagggcggc
     4501 cggcggcacc accggcgcgg ctccccggag gaggcggccg agcgggagcc ccgacgccac
     4561 cgcgcgcacc ggcaccagga tccgagcaag gagtgcgccg gcgccaaggg cgagcggcgc
     4621 gcgcggcacc gcggcggccc ccgagcgggg ccccgggagg cggagagcgg ggaggagccg
     4681 gcgcggcggc accgggcccg gcacaaggcg cagcctgctc acgaggctgt ggagaaggag
     4741 accacggaga aggaggccac ggagaaggag gctgagatag tggaagccga caaggaaaag
     4801 gagctccgga accaccagcc ccgggagcca cactgtgacc tggagaccag tgggactgtg
     4861 actgtgggtc ccatgcacac actgcccagc acctgtctcc agaaggtgga ggaacagcca
     4921 gaggatgcag acaatcagcg gaacgtcact cgcatgggca gtcagccccc agacccgaac
     4981 actattgtac atatcccagt gatgctgacg ggccctcttg gggaagccac ggtcgttccc
     5041 agtggtaacg tggacctgga aagccaagca gaggggaaga aggaggtgga agcggatgac
     5101 gtgatgagga gcggcccccg gcctatcgtc ccatacagct ccatgttctg tttaagcccc
     5161 accaacctgc tccgccgctt ctgccactac atcgtgacca tgaggtactt cgaggtggtc
     5221 attctcgtgg tcatcgcctt gagcagcatc gccctggctg ctgaggaccc agtgcgcaca
     5281 gactcgccca ggaacaacgc tctgaaatac ctggattaca ttttcactgg tgtctttacc
     5341 tttgagatgg tgataaagat gatcgacttg ggactgctgc ttcaccctgg agcctatttc
     5401 cgggacttgt ggaacattct ggacttcatt gtggtcagcg gcgccctggt ggcgtttgct
     5461 ttctcaggat ccaaagggaa agacatcaat accatcaagt ctctgagagt ccttcgtgtc
     5521 ctgcggcccc tcaagaccat caaacggctg cccaagctca aggctgtgtt tgattgtgtg
     5581 gtgaactccc tgaagaatgt cctcaacatc ttgattgtct acatgctctt catgttcata
     5641 tttgccgtca ttgcggtgca gctcttcaaa gggaagtttt tctactgcac agatgaatcc
     5701 aaggagctgg agagggactg caggggtcag tatttggatt atgagaagga ggaagtggaa
     5761 gctcagccca ggcagtggaa gaaatacgac tttcactacg acaatgtgct ctgggctctg
     5821 ctgacgctgt tcacagtgtc cacgggagaa ggctggccca tggtgctgaa acactccgtg
     5881 gatgccacct atgaggagca gggtccaagc cctgggtacc gcatggagct gtccatcttc
     5941 tacgtggtct actttgtggt ctttcccttc ttcttcgtca acatctttgt ggctttgatc
     6001 atcatcacct tccaggagca gggggacaag gtgatgtctg aatgcagcct ggagaagaac
     6061 gagagggctt gcattgactt cgccatcagc gccaaacccc tgacacggta catgccccaa
     6121 aaccggcagt cgttccagta taagacgtgg acatttgtgg tctccccgcc ctttgaatac
     6181 ttcatcatgg ccatgatagc cctcaacact gtggtgctga tgatgaagtt ctacgatgca
     6241 ccctatgagt acgagctgat gctgaaatgc ctgaacatcg tgttcacatc catgttctcc
     6301 atggaatgcg tgctgaagat catcgccttt ggggtgctga actatttcag agatgcctgg
     6361 aatgtctttg actttgtcac tgtgttggga agtattactg atattttagt aacagagatt
     6421 gcggaaacga acaatttcat caacctcagc ttcctccgcc tctttcgagc tgcgcggctg
     6481 atcaagctgc tccgccaggg ctacaccatc cgcatcctgc tgtggacctt tgtccagtcc
     6541 ttcaaggccc tgccctacgt gtgtctgctc attgccatgc tgttcttcat ctacgccatc
     6601 atcggcatgc aggtgtttgg gaatattgcc ctggatgatg acaccagcat caaccgccac
     6661 aacaacttcc ggacgttttt gcaagccctg atgctgctgt tcaggagcgc cacgggggag
     6721 gcctggcacg agatcatgct gtcctgcctg agcaaccagg cctgtgatga gcaggccaat
     6781 gccaccgagt gtggaagtga ttttgcctac ttctacttcg tctccttcat cttcctgtgc
     6841 tcctttctga tgttgaacct ctttgtggct gtgatcatgg acaattttga gtacctcacg
     6901 cgggactctt ccatcctagg tcctcaccac ttggatgagt tcatccgggt ctgggctgaa
     6961 tacgacccgg ctgcgtgtgg gcgcatcagt tacaatgaca tgtttgagat gctgaaacac
     7021 atgtccccgc ctctggggct ggggaagaaa tgccctgctc gagttgctta caagcgcctg
     7081 gttcgcatga acatgcccat ctccaacgag gacatgactg ttcacttcac gtccacgctg
     7141 atggccctca tccggacggc actggagatc aagctggccc cagctgggac aaagcagcat
     7201 cagtgtgacg cggagttgag gaaggagatt tccgttgtgt gggccaatct gccccagaag
     7261 actttggact tgctggtacc accccataag cctgatgaga tgacagtggg gaaggtttat
     7321 gcagctctga tgatattcga cttctacaag cagaacaaaa ccaccagaga ccagatgcag
     7381 caggctcctg gaggcctctc ccagatgggt cctgtgtccc tgttccaccc tctgaaggcc
     7441 accctggagc agacacagcc ggctgtgctc cgaggagccc gggttttcct tcgacagaag
     7501 agttccacct ccctcagcaa tggcggggcc atacaaaacc aagagagtgg catcaaagag
     7561 tctgtctcct ggggcactca aaggacccag gatgcacccc atgaggccag gccacccctg
     7621 gagcgtggcc actccacaga gatccctgtg gggcggtcag gagcactggc tgtggacgtt
     7681 cagatgcaga gcataacccg gaggggccct gatggggagc cccagcctgg gctggagagc
     7741 cagggtcgag cggcctccat gccccgcctt gcggccgaga ctcagcccgt cacagatgcc
     7801 agccccatga agcgctccat ctccacgctg gcccagcggc cccgtgggac tcatctttgc
     7861 agcaccaccc cggaccgccc accccctagc caggcgtcgt cgcaccacca ccaccaccgc
     7921 tgccaccgcc gcagggacag gaagcagagg tccctggaga aggggcccag cctgtctgcc
     7981 gatatggatg gcgcaccaag cagtgctgtg gggccggggc tgcccccggg agaggggcct
     8041 acaggctgcc ggcgggaacg agagcgccgg caggagcggg gccggtccca ggagcggagg
     8101 cagccctcat cctcctcctc ggagaagcag cgcttctact cctgcgaccg ctttgggggc
     8161 cgtgagcccc cgaagcccaa gccctccctc agcagccacc caacgtcgcc aacagctggc
     8221 caggagccgg gaccccaccc acaggccggc tcagccgtgg gctttccgaa cacaacgccc
     8281 tgctgcagag agaccccctc agccagcccc tggcccctgg ctctcgaatt ggctctgacc
     8341 cttacctggg gcagcgtctg gacagtgagg cctctgtcca cgccctgcct gaggacacgc
     8401 tcactttcga ggaggctgtg gccaccaact cgggccgctc ctccaggact tcctacgtgt
     8461 cctccctgac ctcccagtct caccctctcc gccgcgtgcc caacggttac cactgcaccc
     8521 tgggactcag ctcgggtggc cgagcacggc acagctacca ccaccctgac caagaccact
     8581 ggtgctagtc tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca
     8641 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     8701 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     8761 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     8821 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     8881 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     8941 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     9001 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     9061 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     9121 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     9181 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     9241 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     9301 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     9361 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9421 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9481 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     9541 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     9601 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     9661 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     9721 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     9781 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     9841 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     9901 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     9961 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
    10021 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
    10081 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
    10141 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
    10201 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
    10261 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
    10321 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
    10381 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
    10441 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
    10501 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
    10561 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
    10621 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
    10681 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
    10741 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    10801 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
    10861 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
    10921 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    10981 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    11041 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    11101 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    11161 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    11221 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    11281 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    11341 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    11401 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    11461 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    11521 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    11581 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    11641 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    11701 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
    11761 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    11821 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    11881 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    11941 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    12001 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    12061 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    12121 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    12181 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    12241 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    12301 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    12361 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    12421 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    12481 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    12541 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    12601 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    12661 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    12721 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    12781 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    12841 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    12901 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    12961 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    13021 cacatttccc cgaaaagtgc cacctgacgt c
//
