LOCUS       sequence-39212-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  TITLE     Bar-Sagi Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCGN-Sos Cat
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           98..137
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             167..193
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             173..199
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag acaccgggac cgatccagcc tccgggggat cttggtggcg tgaaactccc
      121 gcacctcttc ggccagcgcc ttgtagaagc gcgtatggct tctagctatc cttatgacgt
      181 gcctgactat gccagcctgg gaggaccttc tagagaggag cagatgaggc tgcctagtgc
      241 tgatgtttat agatttgcag agcctgactc tgaagagaat attatatttg aagagaacat
      301 gcagcccaag gctggaattc caattatcaa agcaggaact gttattaaac ttatagagag
      361 gcttacgtac catatgtacg cagatcccaa ttttgttcgg acatttctta caacatacag
      421 atccttttgc aaacctcaag aactactgag tcttataata gaaaggtttg aaattccaga
      481 gcctgagcca acagaagctg atcgcatagc tatagagaat ggagatcaac ccttgagtgc
      541 agaactgaaa agatttagaa aagaatatat acagcctgtg caactgcgag tattaaatgt
      601 atgtcggcac tgggtagagc accacttcta tgattttgaa agagatgcat atcttttgca
      661 acgaatggaa gaatttattg gaacagtaag aggtaaagca atgaaaaaat gggttgaatc
      721 catcactaaa ataatccaaa ggaaaaaaat tgcaagagac aatggaccag gtcataatat
      781 tacatttcag agttcacctc ccacagttga gtggcatata agcagacctg ggcacataga
      841 gactttt
//
