LOCUS       sequence-392122-        2170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 7 part for Modular Cloning in Yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Simakin P, Koch C, Herrmann JM
  TITLE     A modular cloning (MoClo) toolkit for reliable intracellular protein
            targeting in the yeast Saccharomyces cerevisiae.
  JOURNAL   Microb Cell. 2023 Feb 28;10(4):78-87. doi: 10.15698/mic2023.04.794. 
            eCollection 2023 Apr 3.
  PUBMED    37009624
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15698/mic2023.04.794"; journalName: "Microb Cell"; date: 
            "2023-02-28- 28"; volume: "10"; issue: "4"; pages: "78-87"
COMMENT     Sequence Label: pHHYTK123
FEATURES             Location/Qualifiers
     source          1..2170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    14..517
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     terminator      complement(535..569)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(640..1299)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1214..1233
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1481..2069
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1970..1989
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcag agtatcacgt gctataaaaa taattataat ttaaattttt taatataaat
       61 atataaatta aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt tttgttttcc
      121 gaagatgtaa aagactctag ggggatcgcc aacaaatact accttttatc ttgctcttcc
      181 tgctctcagg tattaatgcc gaattgtttc atcttgtctg tgtagaagac cacacacgaa
      241 aatcctgtga ttttacattt tacttatcgt taatcgaatg tatatctatt taatctgctt
      301 ttcttgtcta ataaatatat atgtaaagta cgctttttgt tgaaattttt taaacctttg
      361 tttatttttt tttcttcatt ccgtaactct tctaccttct ttatttactt tctaaaatcc
      421 aaatacaaaa cataaaaata aataaacaca gagtaaattc ccaaattatt ccatcattaa
      481 aagatacgag gcgcgtgtaa gttacaggca agcgatcccg atgagaccag accaataaaa
      541 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
      601 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
      661 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
      721 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
      781 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
      841 gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc ctttagggaa
      901 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
      961 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     1021 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     1081 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     1141 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     1201 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     1261 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     1321 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     1381 ggaacctctt acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1441 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1501 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1561 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1621 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1681 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1741 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1801 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1861 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1921 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1981 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2041 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2101 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2161 ataaccgtag
//
